Assisted Reproductive Technology

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9 Can anyone help me out with a non-invasive technique/procedure to detect early/late pregnancy in pigs?
By Yoya Vashi · Don Bosco University

Franz Schwarzenberger · University of Veterinary Medicine in Vienna

Pregnancy diagnosis in sows by estimation of oestrogens in blood, urine or faeces HS Choi, E Kiesenhofer, H Gantner, J Hois… - Animal Reproduction S... [more]

Pregnancy diagnosis in sows by estimation of oestrogens in blood, urine or faeces HS Choi, E Kiesenhofer, H Gantner, J Hois… - Animal Reproduction Science 15, Issues 3–4, December 1987, Pages 209–216 Abstract Concentrations of oestrone sulphate in blood and of total unconjugated oestrogens in faeces were measured during the oestrous cycle and early gestation. The level of these hormones in blood and faeces was consistently higher (blood, 100%; faeces, 69–100%) between Days 24 and 30 in pregnant than in non-pregnant sows. Therefore, early pregnancy diagnosis based on the concentration of oestrogens in blood, urine and faeces was performed on Day 27, 28 or 29 after service for a total of 290 sows. Blood samples (25 μl) were taken from the tail vein of 289 sows. One hundred urine samples (1 ml) were collected by inserting a cellulose sponge into the vagina and faeces samples (2 g) were taken from the rectum of 290 sows. Pregnancy diagnosis was made on the assumption that more than 2.79 ng oestrone sulphate/ml blood, 19.7 ng oestrogens/g faeces and 65.6 ng oestrogens/ml urine indicated a pregnant animal. The efficiency of the tests was determined by comparing the diagnosis based on oestrogen concentrations with the farrowing results. The oestrone sulphate concentration in blood, as measured by enzyme immunoassay on microtitre plates, showed that 265 of the examined sows were correctly diagnosed, an accuracy of 91.7%. Seven results were false negative and 17 false positive. The determination of oestrogens in urine by radioimmunoassay (RIA) showed that 98 of 100 examined sows were correctly diagnosed and 2 were false negative. According to the oestrogen concentration in faeces, measured by RIA, 271 sows (93.5%) were correctly diagnosed, 16 were false negative and 3 false positive.

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Open Does anybody freeze rabbit spermatozoa with commercial cryopreservation media?
Media developed for rabbit sperm cryopreservation.

Media developed for rabbit sperm cryopreservation.

By Loris Commin · VetAgro Sup

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3 If i have many blastocysts in a petri dish, how can I compare between them and decide which one can I transfer to a patient?
By Eman Abu El-Naga · Animal Reproduction Research Institute

Marc Bergh · Kantonsspital Baden AG + Quartec GmbH

Dear Eman, if you need help visit www.quartec.co

Dear Eman, if you need help visit www.quartec.co

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1 The cut off of 1.5 ng/ml of progesterone per day for triggering IVF stimulation ,is it available to all patients?
By Samira Berbera · TIziri IVF center Algiers

Eric Scott Sills · PRC-Orange County

At our center, the P4 result is just one factor used to determine day of hCG trigger. The ovulation induction protocol matters, as GnRH antagonist IVF... [more]

At our center, the P4 result is just one factor used to determine day of hCG trigger. The ovulation induction protocol matters, as GnRH antagonist IVF cases will evoke a different P4 pattern compared to those receiving standard GnRH agonist downregulation. The value you mention (1.5ng/ml) probably would be a reasonable level, although P4 levels are best interpreted against corresponding E2 levels taken on the same day. Lower E2 responses could be triggered at a lower absolute P4..

http://www.ncbi.nlm.nih.gov/pubmed/22494924 ×
Serum progesterone level effects on the outcom... [Fertil Steril. 2012] - PubMed - NCBI

PubMed comprises more than 22 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

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3 Do patients with premature ovarian failure bleed with a progesterone challenge test?
By Muchabayiwa Gidiri · University of Zimbabwe

Gulab Singh Shekhawat

ovarian failure confirmed by high FSH and LH or Antimullerian factor do not menstruate only with progesterone. If her uterus is normal in HSG than don... [more]

ovarian failure confirmed by high FSH and LH or Antimullerian factor do not menstruate only with progesterone. If her uterus is normal in HSG than donor eggs from other women fertlized by her husband sperms in IVF or ICSI can be best option .

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10 Which one is the best to have the best rate of blastocytes in co-culture: cumulus, vero cells, lymphocytes, or endometrial cells?
By Madkour Aicha · University Mohammed V Agdal

Madkour Aicha · University Mohammed V Agdal

i'm working in my project of thesis about in vitro maturation of oocyte specially for OPK women and i observed during my research that all OPK women e... [more]

i'm working in my project of thesis about in vitro maturation of oocyte specially for OPK women and i observed during my research that all OPK women even with mature oocyte MII after stimulation, those are bad and as consequence they have all embryo 'bad quality' so the important thing is not the quantity but the quality which is so low for those patients ...

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5 Pipette tips
By Ep López-Damián · National Autonomous University of Mexico

Abdul Ahamed · University of Central Lancashire

Pipette tips always disposable we cannot reuse any circumstances, washing is the not the standerd of practice in the Lab

Pipette tips always disposable we cannot reuse any circumstances, washing is the not the standerd of practice in the Lab

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2 Does anybody have experience with primary cell culture of adult buffaloes?
By Islam Saadeldin · Seoul National University

Islam Saadeldin · Seoul National University

Thank you Dr Oliveira for your reply. The cell of interest is the "buffalo fibroblast". My previous work was in the "Cow" but now I am working in the... [more]

Thank you Dr Oliveira for your reply. The cell of interest is the "buffalo fibroblast". My previous work was in the "Cow" but now I am working in the "Buffalo" however the principle is the same, I am wondering if there is something special for the buffalo.

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5 What are the requirements of an IVF laboratory?
By Ep López-Damián · National Autonomous University of Mexico

Marc Bergh · Kantonsspital Baden AG + Quartec GmbH

What Monika Uskoreit says is correct but one has to know that this is a directive and each country has the obligation to transpose this directive in n... [more]

What Monika Uskoreit says is correct but one has to know that this is a directive and each country has the obligation to transpose this directive in national laws which has already resulted in differences between countries. The best example is the discussion arround the use of open and closed systemd to freeze gametes.

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8 Aspects regarding IVF room
By Ep López-Damián · National Autonomous University of Mexico

Marc Bergh · Kantonsspital Baden AG + Quartec GmbH

There are those days product specially developped for embryology labs. I do not sell those products but could provide you advise visit www.quartec.co... [more]

There are those days product specially developped for embryology labs. I do not sell those products but could provide you advise visit www.quartec.co.

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6 Bovine cloning: increasing fusion rate
By Hongbo Liu ·

Hongbo Liu ·

Thanks! I used needle electrodes to do pig cloning.

Thanks! I used needle electrodes to do pig cloning.

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8 new IVF methods
By Irina Ergul · T.C. Süleyman Demirel Üniversitesi

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1 Embryo Biopsy for PGD by Dr.Sherman Silber,MD
By Hussein Almeamar,MD · Putra University, Malaysia

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1 Lipid Peroxidation
By Ep López-Damián · National Autonomous University of Mexico

Alba Torres · Instituto Valenciano de Infertilidad IVI

You can use BODIPY 11 and flow cytometry

You can use BODIPY 11 and flow cytometry

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Assisted Reproductive Technology

Serum progesterone level effects on the outcom... [Fertil Steril. 2012] - PubMed - NCBI

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