Adenoviruses are medium-sized (90–100 nm), nonenveloped (without an outer lipid bilayer) icosahedral viruses composed of a nucleocapsid and a double-stranded linear DNA genome. There are 57 described serotypes in humans, which are responsible for 5–10% of upper respiratory infections in children, and many infections in adults as well.

• James David Londino

  Suppliers for the synthesis of adenoviral vectors?

  I am looking for a company or institution to produce and purify an adenoviral vector for me. I am willing to subclone my gene of interest into an adenoviral plasmid. The insert is relatively small.I am looking for a company or institution to produce and purify an adenoviral vector for me. I am willing to subclone my gene of interest into an adenoviral plasmid. The insert is relatively small. Can anyone suggest a source that is reliable and will do it for a reasonable price?

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    James David Londino replied

    Yeah, I looked at their website. Does the $1250 for "Recombinant Adenovirus Expressing cDNA" include everything after handing them a the plasmid cloned into the vector, up to the point where theyYeah, I looked at their website. Does the $1250 for "Recombinant Adenovirus Expressing cDNA" include everything after handing them a the plasmid cloned into the vector, up to the point where they hand me a stock of adenovirus? Thanks everyone for the feedback.

    2 days ago
• Raewyn Poulsen

  RNAi-mediated gene knockdown in adenoviral-transduced cells

  I work with primary human cells which are difficult to transfect. I am over-expressing a protein using an adenoviral vector. I want to knockdown expression of a second gene in the adenoviral-infectedI work with primary human cells which are difficult to transfect. I am over-expressing a protein using an adenoviral vector. I want to knockdown expression of a second gene in the adenoviral-infected cells however I am having toxicity issues with siRNA. Even with scrambled control siRNA (which is usually non-toxic to my cells) I get massive cell death. I have tried simultaneously introducing the siRNA and the virus as well as introducing the siRNA waiting 48h then infecting with the adenovirus. Mock transfected cells are healthy in both cases. (I haven't tried infecting with the adenovirus, allowing recovery, then introducing the siRNA as the protein I am over-expressing with the adenovirus promotes apoptosis (& the siRNA should prevent this)). Has anyone else encountered this problem or have any ideas as to what might be causing the toxicity?

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    Raewyn Poulsen replied

    Thanks for all your suggestions! I will have another look at lenti (I've only been getting about 30% infection success with lenti compared to about 90% with adeno but sounds like I need to playThanks for all your suggestions! I will have another look at lenti (I've only been getting about 30% infection success with lenti compared to about 90% with adeno but sounds like I need to play around a bit more with lenti.) I like the idea of combining the shRNA with the transgene in the same vector. Thanks so much.

    12 days ago
• Ijad Madisch

  Virology congresses in USA

  Does anyone know some congresses in the field of virology in the U.S or somewhere else? Best Ijad

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    Jose G Teodoro replied

    DNA Tumour Virus Meeting. in Montreal from July 16-22. http://www.union.wisc.edu/dna2012/index.html

    Apr 27, 2012
• Nandhini Bhashini

  Can anyone help me with the primers needed for detecting adenoviruses in a conventional PCR?

  I need just one primer set for detecting it rather than serogroups.

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    Marton Vidovszky replied

    Hi! There are a general nested PCR primers from this paper: Wellehan et al. 2004: http://www.ncbi.nlm.nih.gov/pubmed/15542689, which primers amplify from fish adenovirus to mastadenovirusesHi! There are a general nested PCR primers from this paper: Wellehan et al. 2004: http://www.ncbi.nlm.nih.gov/pubmed/15542689, which primers amplify from fish adenovirus to mastadenoviruses everything. Only the (Platyrrhini) hosts showed problems. But if you need primers for human adenoviruses, you need to use one of the above-mentioned human specific primer pairs.

    Mar 5, 2012
• Fiza Soid

  Does anyone have experience with adenovirus receptor modification using the PEGylation method?

  I cant access some full journal about this method. May somebody help me for the detail method used

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    Fiza Soid replied

    Wow, thank you so much. i will look for it..

    Jan 9, 2011
• Sebastian Darr

  oncogenic persistance in human tissues

  Adenoviruses are known to cause malignancy in rodents. Can anyone provide information concerning oncogenic potential or at least adenoviral persistance in human cancer cells? Maybe some hints for anyAdenoviruses are known to cause malignancy in rodents. Can anyone provide information concerning oncogenic potential or at least adenoviral persistance in human cancer cells? Maybe some hints for any relationship, primary data like from a digital transcriptome subtraction or something similar? Thanks a lot, Sebastian

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    David Ornelles replied

    I think the work of Thomas Dobner has established the possibility that adenovirus products elicit mutations in a hit-and-run fashion. This means adenovirus can transform cells with no persistentI think the work of Thomas Dobner has established the possibility that adenovirus products elicit mutations in a hit-and-run fashion. This means adenovirus can transform cells with no persistent presence. As of yet, I know of no evidence for such a phenomenon in humans. However, work of Thomas Lion has suggested what may be a link between the persistence of adenovirus gene expression and cancer. They found a curious connection between adenovirus and certain pediatric brain tumors (PMID: 17494079). The fact that 293 and 911 cells have a neural lineage (see the work of Shaw. PMID: 11967234) and the relative ease with which adenovirus products can transform human cells of neural origins makes one think that sequence mining in other pediatric brain tumors may be informative.

    May 23, 2010
• Ijad Madisch

  Experiences

  Hey, a lot of researchers are using adenoviral vectors for gene therapy. Nevertheless, there are no overviews, which adenoviral type would be best suitable for a specific tissue. WhichHey, a lot of researchers are using adenoviral vectors for gene therapy. Nevertheless, there are no overviews, which adenoviral type would be best suitable for a specific tissue. Which experiences do you have? Best Ijad

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