W O H Hughes

Publications (15)44.41 Total impact

• Article: Size and asymmetry: are there costs to winning the royalty race?

  R E Mitchell, C L Frost, W O H Hughes
  [show abstract] [hide abstract]
  ABSTRACT: Body size and morphology are key fitness-determining traits that can vary genotypically. They are likely to be important in social insect queens, which mate in swarms and found colonies independently, but genetic influences on queen morphology have been little investigated. Here, we show that the body size and morphology of queens are influenced by their genotype in the leaf-cutting ant Acromyrmex echinatior, a species in which certain lineages (patrilines) bias their development towards reproductive queens rather than sterile workers. We found no relationship between the queen-worker skew of patrilines and the size or morphology of queens, but there was a significant relationship with fluctuating asymmetry, which was greater in more queen-biased patrilines. Our results suggest that queen-biased patrilines do not incur a fitness cost in terms of body size, but may face more subtle costs in developmental stability. Such costs may constrain the evolution of royal cheating in social insects.
  Journal of Evolutionary Biology 03/2012; 25(3):522-31. · 3.28 Impact Factor
• Article: Multiple gains and losses of Wolbachia symbionts across a tribe of fungus-growing ants.

  C L Frost, H Fernández-Marín, J E Smith, W O H Hughes
  [show abstract] [hide abstract]
  ABSTRACT: Although the intracellular bacterium Wolbachia is ubiquitous in insects, it has a unique relationship with New World ants on which particular bacterial strains have specialized. However, data are from distantly related hosts and detailed phylogenetic information which could reveal transmission dynamics are lacking. Here, we investigate host-Wolbachia relationships in the monophyletic fungus-growing ant tribe Attini, screening 23 species and using multilocus sequence typing to reliably identify Wolbachia strains. This technique reduces the significant problem of recombination seen using traditional single gene techniques. The relationship between Wolbachia and the fungus-growing ants appears complex and dynamic. There is evidence of co-cladogenesis, supporting vertical transmission; however, this is incomplete, demonstrating that horizontal transmission has also occurred. Importantly, the infection prevalence is frequently different between closely related taxa, with the Acromyrmex leaf-cutting ants appearing particularly prone to infection and there being no consistent relationship with any of the major life history transitions. We suggest that infection loss and horizontal transmission have driven epidemics or selective sweeps of Wolbachia, resulting in multiple gains and losses of infection across the fungus-growing ants.
  Molecular Ecology 09/2010; 19(18):4077-85. · 5.52 Impact Factor
• Source

  Available from: PubMed Central

  Article: Caste-specific expression of genetic variation in the size of antibiotic-producing glands of leaf-cutting ants.

  W O H Hughes, A N M Bot, J J Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: Social insect castes represent some of the most spectacular examples of phenotypic plasticity, with each caste being associated with different environmental conditions during their life. Here we examine the level of genetic variation in different castes of two polyandrous species of Acromyrmex leaf-cutting ant for the antibiotic-producing metapleural gland, which has a major role in defence against parasites. Gland size increases allometrically. The small workers that play the main role in disease defence have relatively large glands compared with larger workers, while the glands of gynes are substantially larger than those of any workers, for their body size. The gland size of large workers varies significantly between patrilines in both Acromyrmex echinatior and Acromyrmex octospinosus. We also examined small workers and gynes in A. echinatior, again finding genetic variation in gland size in these castes. There were significant positive relationships between the gland sizes of patrilines in the different castes, indicating that the genetic mechanism underpinning the patriline variation has remained similar across phenotypes. The level of expressed genetic variation decreased from small workers to large workers to gynes. This is consistent with the hypothesis that there is individual selection on disease defence in founding queens and colony-level selection on disease defence in the worker castes.
  Proceedings of the Royal Society B: Biological Sciences 10/2009; 277(1681):609-15. · 5.41 Impact Factor
• Article: Multi-level selection for hygienic behaviour in honeybees.

  J A Pérez-Sato, N Châline, S J Martin, W O H Hughes, F L W Ratnieks
  [show abstract] [hide abstract]
  ABSTRACT: Disease is one of the main factors driving both natural and artificial selection. It is a particularly important and increasing threat to the managed honeybee colonies, which are vital in crop pollination. Artificial selection for disease-resistant honeybee genotypes has previously only been carried out at the colony-level, that is, by using queens or males reared from colonies that show resistance. However, honeybee queens mate with many males and so each colony consists of multiple patrilines that will vary in heritable traits, such as disease resistance. Here, we investigate whether response to artificial selection for a key resistance mechanism, hygienic behaviour, can be improved using multi-level selection, that is, by selecting not only among colonies as normal but also among patrilines within colonies. Highly hygienic colonies were identified (between-colony selection), and the specific patrilines within them responsible for most hygienic behaviour were determined using observation hives. Queens reared from these hygienic patrilines (within-colony selection) were identified using DNA microsatellite analysis of a wing-tip tissue sample and then mated to drones from a third highly hygienic colony. The resulting colonies headed by queens from hygienic patrilines showed approximately double the level of hygienic behaviour of colonies headed by sister queens from non-hygienic patrilines. The results show that multi-level selection can significantly improve the success of honeybee breeding programs.
  Heredity 04/2009; 102(6):609-15. · 4.60 Impact Factor
• Article: Multiple paternity or multiple queens: two routes to greater intracolonial genetic diversity in the eusocial Hymenoptera.

  W O H Hughes, F L W Ratnieks, B P Oldroyd
  [show abstract] [hide abstract]
  ABSTRACT: Understanding the evolution of multiple mating by females (polyandry) is an important question in behavioural ecology. Most leading explanations for polyandry by social insect queens are based upon a postulated fitness benefit from increased intracolonial genetic diversity, which also arises when colonies are headed by multiple queens (polygyny). An indirect test of the genetic diversity hypotheses is therefore provided by the relationship between polyandry and polygyny across species, which should be negative if the genetic diversity hypotheses are correct. Here, we conduct a powerful comparative investigation of the relationship between polyandry and polygyny for 241 species of eusocial Hymenoptera (ants, bees and wasps). We find a clear and significant negative relationship between polyandry and polygyny after controlling for phylogeny. These results strongly suggest that fitness benefits resulting from increased intracolonial genetic diversity have played an important role in the evolution of polyandry, and possibly polygyny, in social insects.
  Journal of Evolutionary Biology 08/2008; 21(4):1090-5. · 3.28 Impact Factor
• Source

  Available from: Michael Poulsen

  Article: Differential resistance and the importance of antibiotic production in Acromyrmex echinatior leaf-cutting ant castes towards the entomopathogenic fungus Aspergillus nomius

  M. Poulsen, W. O. H. Hughes, J. J. Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: Paired exocrine metapleural glands are present in almost all ants and produce compounds with antibiotic properties towards a variety of pathogenic fungi and bacteria. In Acromyrmex leaf-cutting ants, small workers have relatively large metapleural glands compared to large workers, and thus harbour approximately half the number of gland cells of large workers, despite being only one-fifteenth their body mass. Here we present results showing that when the two worker castes of A. echinatior are treated with spores of the pathogenic fungus Aspergillus nomius in doses that correspond to the difference in metapleural gland cell numbers they do not differ in survival. However, we also show, for the first time, that small workers survive significantly longer than large workers when both are challenged with a dose of spores that corresponds to their difference in body mass. Furthermore, the time until Aspergillus nomius hyphae and spores appear on the cadavers of workers dead from infection, is significantly increased in the small worker caste. In addition to supporting previous findings that the metapleural glands have an important defence function, the results of this study indicate that the relatively large glands in small workers makes this caste particularly well adapted to preventing pathogenic microorganisms from entering the colony.
  Insectes Sociaux 07/2006; 53(3):349-355. · 1.70 Impact Factor
• Source

  Available from: ku.dk

  Article: Does genetic diversity hinder parasite evolution in social insect colonies?

  W O H Hughes, J J Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: Polyandry is often difficult to explain because benefits of the behaviour have proved elusive. In social insects, polyandry increases the genetic diversity of workers within a colony and this has been suggested to improve the resistance of the colony to disease. Here we examine the possible impact of host genetic diversity on parasite evolution by carrying out serial passages of a virulent fungal pathogen through leaf-cutting ant workers of known genotypes. Parasite virulence increased over the nine-generation span of the experiment while spore production decreased. The effect of host relatedness upon virulence appeared limited. However, parasites cycled through more genetically diverse hosts were more likely to go extinct during the experiment and parasites cycled through more genetically similar hosts had greater spore production. These results indicate that host genetic diversity may indeed hinder the ability of parasites to adapt while cycling within social insect colonies.
  Journal of Evolutionary Biology 02/2006; 19(1):132-43. · 3.28 Impact Factor
• Article: Examination of the immune responses of males and workers of the leaf-cutting ant Acromyrmex echinatior and the effect of infection

  B. Baer, A. Krug, J. J. Boomsma, W. O. H. Hughes
  [show abstract] [hide abstract]
  ABSTRACT: Parasites represent significant challenges to social insects. The high density, interaction rate and relatedness of individuals within colonies are all predicted to make social insect colonies particularly vulnerable to parasites. To cope with this pressure, social insects have evolved a number of defence mechanisms. These include the immune response, which, aside from in bumblebees, has been relatively little studied in social insects. Here we compare the immune responses of males and workers of the leaf-cutting ant Acromyrmex echinatior and examine the effect upon immunocompetence of prior exposure to a virulent parasite. Males have a far lower immune response than workers, suggesting either haploid susceptibility or reduced investment in immunity by the short-lived males. There was also significantly less variation in the immune response of males than of workers, which may be due to leaf-cutting ant workers being more variable in age or more genetically diverse within colonies. When exposed to the entomopathogenic fungus Metarhizium, workers expressed a substantially reduced immune response 96 h after infection, suggesting that the immune system was either depleted by having to respond to the Metarhizium infection or was depressed by the parasite. The results suggest that the immune response is a costly and limited process, but further experiments are needed to distinguish between the alternative explanations for the effects observed.
  Insectes Sociaux 01/2005; 52(3):298-303. · 1.70 Impact Factor
• Source

  Available from: royalsocietypublishing.org

  Article: Let your enemy do the work: within-host interactions between two fungal parasites of leaf-cutting ants.

  W O H Hughes, J J Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: Within-host competition is an important factor in host-parasite relationships, yet most studies consider interactions involving only single parasite species. We investigated the interaction between a virulent obligate entomopathogenic fungus, Metarhizium anisopliae var. anisopliae, and a normally avirulent, opportunistic fungal pathogen, Aspergillus flavus, in their leaf-cutting ant host, Acromyrmex echinatior. Surprisingly, the latter normally out-competed the former in mixed infections and had enhanced fitness relative to when infecting in isolation. The result is most probably due to Metarhizium inhibiting the host's immune defences, which would otherwise normally prevent infections by Aspergillus. With the host defences negated by the virulent parasite, the avirulent parasite was then able to out-compete its competitor. This result is strikingly similar to that seen in immunocompromised vertebrate hosts and indicates that avirulent parasites may play a more important role in host life histories than is generally realized.
  Proceedings of the Royal Society B: Biological Sciences 03/2004; 271 Suppl 3:S104-6. · 5.41 Impact Factor
• Source

  Available from: ku.dk

  Article: Diversity of entomopathogenic fungi near leaf-cutting ant nests in a neotropical forest, with particular reference to Metarhizium anisopliae var. anisopliae.

  W O H Hughes, L Thomsen, J Eilenberg, J J Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: We investigated the prevalence of entomopathogenic fungi associated with leaf-cutting ant colonies in a small area of tropical forest in Panama. There was a high abundance of Metarhizium anisopliae var. anisopliae near the colonies. Beauveria bassiana was also detected in the soil, Aspergillus flavus in dump material, and six Camponotus atriceps ants were found infected with Cordyceps sp. Based on a partial sequence of the IGS region, almost all of the M. anisopliae var. anisopliae isolates fell within one of the three main clades of M. anisopliae var. anisopliae, but with there still being considerable diversity within this clade. The vast majority of leaf-cutting ants collected were not infected by any entomopathogenic fungi. While leaf-cutting ants at this site must, therefore, regularly come into contact with a diversity of entomopathogenic fungi, they do not appear to be normally infected by them.
  Journal of Invertebrate Pathology 02/2004; 85(1):46-53. · 2.06 Impact Factor
• Source

  Available from: Dave Goulson

  Article: The use of alarm pheromones to enhance bait harvest by grass-cutting ants.

  W O H Hughes, D Goulson
  [show abstract] [hide abstract]
  ABSTRACT: The enhancement of bait for the control of grass-cutting ants was investigated using two species of grass-cutting ant, Atta bisphaerica (Forel) and Atta capiguara (Gonçalves) (Hymenoptera: Formicidae). Bait was applied in loose piles to obtain a direct relationship between ant attraction and bait harvest. Enhancement with alarm pheromone compounds significantly increased the attractiveness and harvest of bait under certain conditions. A large proportion of the ants attracted to the enhanced bait were minor workers. These ants rarely transport bait because of their small size, and so it may be possible to increase the effect of bait enhancement by using smaller bait granules. Foragers of A. capiguara were less inclined to transport citrus-pulp bait than were those of Atta laevigata (Fr. Smith), a species that also harvests dicotyledonous plants. This emphasizes the importance of developing a bait matrix that is more acceptable to grass-cutting species. Nevertheless, the results suggest that alarm pheromone compounds have significant potential to improve the efficacy of baits for the control of grass-cutting ants.
  Bulletin of Entomological Research 07/2002; 92(3):213-8. · 1.88 Impact Factor
• Source

  Available from: Dave Goulson

  Article: Polyethism and the importance of context in the alarm reaction of the grass-cutting ant, Atta capiguara

  W.O.H. Hughes, D. Goulson
  [show abstract] [hide abstract]
  ABSTRACT: Leaf-cutting ants exhibit an aggressive alarm response. Yet in most alarm reactions, not all of the ants encountering a disturbance will respond. This variability in behaviour was investigated using field colonies of Atta capiguara, a grass-cutting species. Crushed ant heads were applied near foraging trails to stimulate alarm reactions. We found that minor workers were disproportionately likely to respond. Only 34.7&#452.8% of ants travelling along foraging trails were minor workers, but 82.1&#456.1% of ants that responded were minors. Workers transporting grass did not respond at all. The alarm response was strongest at the position and time where minors were most abundant. Ants were more likely to respond when they were travelling along trails with low rather than high traffic. Minor workers followed a meandering route along the trail, compared with the direct route taken by foragers. We argue that an important function of minor workers on foraging trails is to patrol the trail area for threats, and that they then play the key role in the alarm reaction.
  Behavioral Ecology and Sociobiology 04/2001; 49(6):503-508. · 3.18 Impact Factor
• Source

  Available from: Dave Goulson

  Article: Fly populations associated with landfill and composting sites used for household refuse disposal

  D. Goulson, W.O.H. Hughes, J.W. Chapman
  [show abstract] [hide abstract]
  ABSTRACT: Calyptrate fly populations were monitored with sticky traps at the following sites in Hampshire, UK during August to November 1998: a landfill and composting site (Paulsgrove), a site adjacent to this landfill (Port Solent), a site with no landfill nearby (Gosport), and a composting site with no landfill nearby. Overall, house flies Musca domestica(Linnaeus) and lesser house flies Fannia spp. were not important constituents of the dipteran catch, while bluebottles (Calliphora spp.) and greenbottles (Lucilia spp.) comprised approximately 12% of the total. Very large fly populations were found at the two composting sites, and it seems likely that these provide ideal breeding grounds for a range of fly species since they offer an abundance of warm decaying organic matter. Large fly populations were also evident at the landfill site. The suitability of household waste for the development of calyptrate Diptera was confirmed in a controlled trial: a mean of 0.43 adults emerged per kilo of one-week-old waste. Since many hundreds of tonnes of waste are delivered to the landfill daily, it is clear that the landfill is likely to substantially increase the local population of calyptrate flies. However, the data suggest that there was little movement of Diptera from the landfill to Port Solent situated approximately 500 m away. The most important calyptrate flies at this site were the cluster flies Pollenia rudis (Fabricius) and P. amentaria (Scopoli); the landfill site is unlikely to provide a suitable breeding site for these flies, as the larvae develop as parasites of earthworms. Significantly more flies emerged from one-week-old than from two-week-old household waste. A comparison of different barriers to the emergence of adult house flies from waste demonstrated that sacking provided an effective barrier to fly emergence, but that soil did not differ significantly from control treatments. If managed appropriately, it seems that the use of sacking over landfill waste could substantially reduce associated fly populations.
  Bulletin of entomological research 11/1999; 89(06):493 - 498. · 1.58 Impact Factor
• Source

  Available from: lasi.group.shef.ac.uk

  Article: The epizootiology of a Metarhizium infection in mini-nests of the leaf-cutting ant Atta sexdens rubropilosa

  D.B. Jaccoud, W.O.H. Hughes, C.W. Jackson
  [show abstract] [hide abstract]
  ABSTRACT: There is growing interest in the use of entomopathogenic organisms to control leaf-cutting ants (Hymenoptera: Formicidae: Attini). However, the way leaf-cutting ants react as a colony to biohazards is poorly understood. We investigated the effects of Metarhizium anisopliae (Metschnikoff) (Deuteromycotina: Hyphomycetes) applied to the foraging arenas of mini-nests (queenless sub-colonies) of the leaf-cutting ant Atta sexdens rubropilosa (Forel). Dry spores were applied either alone or mixed with citrus powder, at 0.5g or 0.05g per mini-nest. The spores were removed four days after application, and all dead ants removed every three days. Ant numbers near the Metarhizium increased as the ants attempted to clean up the biohazard. The ants attempted to place the spores in piles, which they then covered over with other material. They were able to deal with the low doses in this way, but the high doses overwhelmed them. All treated mini-nests suffered increased ant mortality during the first ten days after application. This mortality was particularly high in the media worker caste which had played the major role in attempting to clean up the spores. Foraging activity decreased, as did the health of the fungus gardens. The mini-nests exposed to the low dose of spores mixed with citrus powder then recovered fully. The health of the other treated mini-nests declined gradually until around 26 days after application, when they began deteriorating sharply. However, the decline of these mini-nests after day26 was not due directly to the pathogenic action of the Metarhizium, nor to the initial ant mortality it had caused. The results suggest that the social stress caused by even such a short-lived Metarhizium epizootic was sufficient to cause the decline and ultimate death of the mini-nests. This has important implications for the control of leaf-cutting ants. It also demonstrates how important the social homeostasis of the colony is to leaf-cutting ants.
  Entomologia Experimentalis et Applicata 09/1999; 93(1):51-61. · 1.53 Impact Factor
• Article: Diversity of entomopathogenic fungi near leaf-cutting ant nests in a neotropical forest, with particular reference to Metarhizium anisopliae var. anisopliae

  W.O.H. Hughes, L. Thomsen, J. Eilenberg, J.J. Boomsma
  [show abstract] [hide abstract]
  ABSTRACT: We investigated the prevalence of entomopathogenic fungi associated with leaf-cutting ant colonies in a small area of tropical forest in Panama. There was a high abundance of Metarhizium anisopliae var. anisopliae near the colonies. Beauveria bassiana was also detected in the soil, Aspergillus flavus in dump material, and six Camponotus atriceps ants were found infected with Cordyceps sp.. Based on a partial sequence of the IGS region, almost all of the M. anisopliae var. anisopliae isolates fell within one of the three main clades of M. anisopliae var. anisopliae, but with there still being considerable diversity within this clade. The vast majority of leaf-cutting ants collected were not infected by any entomopathogenic fungi. While leaf-cutting ants at this site must, therefore, regularly come into contact with a diversity of entomopathogenic fungi, they do not appear to be normally infected by them.
  Journal of Invertebrate Pathology.