Tatsuji Hataya

Publications (2)2.8 Total impact

• Source

  Available from: paran.com

  Article: Multiple citrus viroids in citrus from Japan and their ability to produce exocortis-like symptoms in citron.

  Takao Ito, Hiroyuki Ieki, Katsumi Ozaki, Toru Iwanami, Kenji Nakahara, Tatsuji Hataya, Tsutae Ito, Masahiro Isaka, Takeshi Kano
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  ABSTRACT: ABSTRACT Sequential polyacrylamide gel electrophoresis analyses showed many viroid-like RNAs in samples collected from citrus trees in Japan. Reverse transcription polymerase chain reaction and sequencing analyses of the amplified fragments verified that they were derived from variants of six citrus viroids, Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd) including CVd-I-LSS (a distinct variant of CBLVd), Hop stunt viroid, Citrus viroid III, Citrus viroid IV, and Citrus viroid OS. The samples induced symptoms with variable severity in Arizona 861-S1 'Etrog' citrons (Citrus medica L.) likely due to the varying accumulation patterns produced by the different viroids. Some of the symptoms caused by the samples harboring the citrus viroids other than CEVd were as severe as those caused by CEVd. Some source citrus trees showing the severe bark scaling characteristic of exocortis disease in trifoliate orange (Poncirus trifoliata (L.) Raf.) rootstocks contained only citrus viroids other than CEVd in complex. This indicates that certain exocortis-like diseases in Japan were caused by some combination of citrus viroids not including CEVd.
  Phytopathology 06/2002; 92(5):542-7. · 2.80 Impact Factor
• Article: Comparisons of gene diagnostic methods for the practical diagnosis of chrysanthemum stunt viroid in chrysanthemum plants

  Tatsuji Hataya, Kenji Nakahara, Kazuyoshi Furuta, Eishiro Shikata
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  ABSTRACT: The sensitivity of gene diagnostic methods for detecting chrysanthemum stunt viroid (CSVd) in 2 M LiCl‐soluble nucleic acids extracted from chrysanthemum plants was examined. Dot blot hybridization using DIG‐labeled cRNA and cDNA probes was compared. The cRNA probe was at least 25 times more sensitive than the cDNA probe and had a superior signal‐to‐noise ratio. Although reverse transcription and polymerase chain reaction (RT‐PCR) is much more sensitive in theory, in practice it was only 5 times more sensitive than hybridization using the cRNA probe. In addition, the nucleic acid extracts used inhibited the cDNA amplifications, probably due to natural inhibitors such as polysaccharides and polyphenolic compounds in the plants. HC1 treatment following ethanol precipitation of the nucleic acid extracts was effective in rapidly eliminating the inhibitory factors. The combination of RT‐PCR and hybridization on microplate well expanded the detectable range and was at least 25 times more sensitive than agarose gel analysis of the RT‐PCR products. Based on these results, the use of practical diagnosis for protecting chrysanthemum from CSVd and for certifying CSVd‐free chrysanthemum is discussed.
  Archives of Phytopathology and Plant Protection 08/1999; 32(3):179-192.