Bong Rae Cho

Korea University, Sŏul, Seoul, South Korea

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Publications (145)552.22 Total impact

  • Mi‐Yun Jeong, Bong Rae Cho
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    ABSTRACT: We summarize the nonlinear optical (NLO) properties of octupolar molecules, crystals, and films developed in our laboratory. We present the design strategy, structure–property relationship, and second-order NLO properties of 1,3,5-trinitro- and 1,3,5-tricyano-2,4,6-tris(p-diethylaminostyryl)benzene (TTB) derivatives, TTB crystals, and films prepared by free-casting TTB in poly(methyl methacrylate) (PMMA). The first hyperpolarizability of TTB was fivefold larger than that of the dipolar analogue. Moreover, the TTB crystal showed unprecedentedly large second-harmonic generation (SHG). While TTB crystal films (20 wt% TTB/PMMA) on various substrates showed appreciable SHG values, the cylinder film exhibited much larger SHG values and large electro-optic (EO) coefficients. The large SHG values and EO coefficients, as well as the high thermal stability of the cylinder film, will make it a potential candidate for NLO device applications.
    The Chemical Record 11/2014; · 4.38 Impact Factor
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    ABSTRACT: A two-photon probe for near-membrane zinc ions that can be excited by 800 nm femtosecond laser pulses and emit at 500 nm is reported. The probe can be easily loaded into cells and tissue slices to visualize near-membrane zinc ions in live cells and tissues for a long period of time through the use of two-photon microscopy.
    Asian Journal of Organic Chemistry. 07/2014;
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    ABSTRACT: Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu(2+) concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu(2+) and can quantitatively estimate the Cu(2+) concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu(2+) concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.
    Analytical Chemistry 05/2014; · 5.70 Impact Factor
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    ABSTRACT: We report two-photon probes for Zn(2+) ions that can simultaneously detect cytosolic and mitochondrial Zn(2+) ions in live cells and living tissues at 115 mm depth by dual-color TPM imaging with minimum interference from other biologically relevant species.
    Organic & Biomolecular Chemistry 04/2014; · 3.57 Impact Factor
  • Hoon Jai Chun, Eun Sun Kim, Bong Rae Cho
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    ABSTRACT: We briefly describe the advantages and limitations of label-free multiphoton microscopy and probe-labeled two-photon microscopy for the endomicroscopic diagnosis. The two methods are complementary and more information can be collected from tissues by combining the two methods. Therefore, parallel efforts should be directed to the development of both label-free MPM and probe-labeled TPM as the diagnostic tool. SCANNING 9999:1-3, 2013. © Wiley Periodicals, Inc.
    Scanning 12/2013; · 1.29 Impact Factor
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    ABSTRACT: Two-photon microscopy (TPM) is a new imaging tool that can detect biological targets deep inside a live tissue. To faciltate the use of TPM in biomedical research, a variety of two-photon (TP) probes for specific applications are needed. In this Forum Article, we describe the design strategy, photophysical properties, and biological imaging applications of a selection of our recent studies in the development of TP probes for metal ions. Small-molecule TP turn-on probes, organelle-targeted probes, and multicolor emissive probes for dual-color imaging are briefly reviewed.
    Inorganic Chemistry 12/2013; · 4.59 Impact Factor
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    ABSTRACT: Hydrogen sulfide (H2S) is a new member of gaseous transmitters, which protects various organs from oxidative stress. In this article, we report a ratiometric two-photon probe, TFCA, which can be excited by 750 nm femtosecond pulses, shows a 110-fold increase in the intensity ratio upon reaction with HS(-) and high selectivity for HS(-), and can visualize the total sulfide ([H2S] + [HS(-)]) distribution in live tissue by two-photon microscopy (TPM). We also developed a kinetic method to quantitatively estimate the total sulfide concentration ([H2S] + [HS(-)]) in live tissues. The kinetic method allowed us to measure the observed rate constants (kobs) for the sulfide-induced deazidation reaction of TFCA in live cells and tissues using TPM. The total sulfide concentration was calculated by using kobs = k2[HS(-)], with the k2 value determined in HEPES/EtOH (1/1, pH = 7.2), and [H2S]/[HS(-)] = [H(+)]/Ka. The total sulfide concentration was found to be nearly zero in HeLa cells and 4-7 µM in rat colon tissues.
    Analytical Chemistry 09/2013; · 5.70 Impact Factor
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    ABSTRACT: Hydrogen sulfide (H2S) is a multifunctional signaling molecule that exerts neuroprotective effects in oxidative stress. In this article, we report a mitochondria-localized two-photon probe (SHS-M2) that can be excited by 750 nm femtosecond pulses and ratiometrically detect H2S in live astrocytes and living brain slices using two-photon microscopy (TPM). SHS-M2 showed a bright two-photon excited fluorescence and a marked blue-to-yellow emission color change in response to H2S, low cytotoxicity with easy loading and minimum interference from other biologically relevant species including reactive sulfur, oxygen and nitrogen species, thereby allowing quantitative analysis of H2S level. Molecular TPM imaging with SHS-M2 in astrocytes reveals that there is a correlation between the ratiometric analysis and expression levels of cystathionine β-synthase (CBS), the major enzyme that catalyzes H2S production. In comparison to wild-type controls, the attenuated H2S production was observed in DJ-1 (a Parkinson's disease gene) knockout astrocytes and brain slices where CBS expression is decreased. These findings demonstrate that reduced H2S levels in astrocytes may contribute to the development of Parkinson's disease (PD), and SHS-M2 may be useful as a marker to detect a risk of neurodegenerative diseases including PD.
    Journal of the American Chemical Society 06/2013; · 10.68 Impact Factor
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    Chang Su Lim, Bong Rae Cho
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    ABSTRACT: Two-photon microscopy (TPM), which uses two photons of lower energy as the excitation source, is a vital tool in biology and clinical science, due to its capacity to image deep inside intact tissues for a long period of time. To make TPM a more versatile tool in biomedical research, we have developed a variety of two-photon probes for specific applications. In this mini review, we will briefly discuss two-photon probes for lipid rafts, lysosomes, mitochondria, and pH, and their biomedical applications. [BMB Reports 2013; 46(4): 188-194].
    BMB reports 04/2013; 46(4):188-94. · 1.63 Impact Factor
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    Hwan Myung Kim, Bong Rae Cho
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    ABSTRACT: Mitochondria provide the energy of the cells and are the primary site of oxygen consumption and the major source of reactive oxygen species. In mitochondria, metal ions and glutathione play vital roles in maintaining their structure and the redox environment. To understand their roles in mitochondria, it is crucial to monitor each of these chemical species in the mitochondria at the cell, tissue, and organism levels. An ideal tool for such purpose is the use of two-photon microscopy (TPM). Until recently, however, there has been no report on the two-photon (TP) probes suitable for such applications. In this paper, we summarize the mitochondria-targeted TP probes for Zn(2+), H(2)O(2), and thiols, as well as their bioimaging applications.
    Oxidative Medicine and Cellular Longevity 01/2013; 2013:323619.
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    Bong Rae Cho, Sang Yong Pyun
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    ABSTRACT: Elimination reactions of (1a-e) promoted by in 70 mol % MeCN(aq) have been studied kinetically. The reactions are second-order and exhibit Brnsted = 0.80-0.84 and = 0.39-0.42, respectively. For all leaving groups and bases employed in this study, the and values remained almost the same. The results can be described by a negligible interaction coefficient, , which describes the interaction between the base catalyst and the leaving group. The negligible pxy interaction coefficient is consistent with the mechanism. Change of the base-solvent system from /MeCN to -70 mol % MeCN(aq) changed the reaction mechanism from E2 to . Noteworthy was the relative insensitivity of the transition state structure to the reaction mechanism change.
    Bulletin- Korean Chemical Society 01/2013; 34(4). · 0.84 Impact Factor
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    Sang Yong Pyun, Bong Rae Cho
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    ABSTRACT: Reactions of 4-nitrophenyl 2-thiophenecarboxylate (1a-e) with in 20 mol % DMSO (aq) have been studied kinetically. The order kinetics, = 0.88-0.98, and linear Hammett and Yukawa-Tsuno plots observed for these reactions indicate an addition-elimination mechanism in which the step is rate limiting. The value increased with a stronger electron-withdrawing 5-thienyl substituent, the Hammett plots are linear except for X = MeO, and Yukawa-Tsuno plots are linear with = 0.79-1.32 and r = 0.28-0.93, respectively. The value increased and r value decreased with a stronger nucleophile, indicating an increase in the electron density at the C=O bond and a decrease in the resonance demand. These results have been interpreted with enhanced N-C bond formation in the transition state with the reactivity increase.
    Bulletin- Korean Chemical Society 01/2013; 34(7). · 0.84 Impact Factor
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    ABSTRACT: Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein (P-gp), caveolin-1 and serum deprivation protein response (SDPR)/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.
    Journal of Proteome Research 12/2012; · 5.06 Impact Factor
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    ABSTRACT: Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity.
    Chemistry 10/2012; · 5.93 Impact Factor
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    ABSTRACT: Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170 GM (GM: 10(-50)  cm(4)  photon(-1) ) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180 μm depth for longer than 1200 s through the use of TPM, with minimum interference from other biologically relevant species.
    Chemistry 08/2012; 18(39):12388-94. · 5.93 Impact Factor
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    ABSTRACT: Establishing probe-based analysis is important for developing multi-photon microscopy (MPM) to make an early diagnosis of colon neoplasm and assess its antioxidant status. Cu(I) and Zn(II) ions are trace elements which roll as cofactors of antioxidant, superoxide dismutase. However, there have been no reports on the features of MPM image using probe of Cu(I) and Zn(II) ions. Our main objective in this study was application of newly developed multi-photon probe (MP) probe on Cu(I) and Zn(II) ions as a tool to assess antioxidative status of colon neoplasm. This study was a pilot study. Colon cancer cell lines (ACT116 and HT-29), NIH3T3 cells and tissues of normal colon mucosa and colon neoplasm obtained during colonoscopic biopsy from 17 patients were stained with MP probes for Cu(I) and Zn(II) (ACu1 and AZn1). Cu(I)/Zn(II) levels in the cells and tissues were determined by detecting MP-excited fluorescence by MPM. MPM images of cells stained with MP probes revealed that Cu(I) was more abundant in ACT116 and HT-29 cells than in NIH3T3 cells, while Zn(II) was more abundant in NIH3T3 cells than in ACT116 and HT-29 cells. Normal tissues had a defined texture, whereas adenoma/adenocarcinoma tissues were amorphous. The level of Cu(I) increased and that of Zn(II) decreased with the transition from normal to adenoma to adenocarcinoma tissue. MPM can be used to determine the relative Cu(I)/Zn(II) levels in cells and colon tissues by using ACu1 and AZn1 as MPM probes. These finding are new research tools for gastroenterologists to assess antioxidant status of colon neoplasm.
    Journal of clinical pathology 07/2012; 65(10):882-7. · 2.43 Impact Factor
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    ABSTRACT: We report a two-photon probe (FS1) which shows a 21-fold two-photon excited fluorescence enhancement in response to H(2)S and can selectively detect H(2)S in a rat hippocampal slice at a depth of 90-190 μm by using two-photon microscopy.
    Chemical Communications 07/2012; 48(67):8395-7. · 6.38 Impact Factor
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    ABSTRACT: We report a highly sensitive two-photon probe (SZn2-Mito) which shows a 70-fold two-photon excited fluorescence enhancement in response to Zn(2+) and can selectively detect mitochondrial Zn(2+) in a rat hippocampal slice at a depth of 100-200 μm by using two-photon microscopy.
    Chemical Communications 03/2012; 48(38):4546-8. · 6.38 Impact Factor
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    ABSTRACT: We report a two-photon fluorescent probe (SHP-Mito) which can ratiometrically detect mitochondrial H(2)O(2) in live cells and intact tissues at >100 μm depth through the use of two-photon microscopy.
    Chemical Communications 03/2012; 48(29):3518-20. · 6.38 Impact Factor
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    ABSTRACT: Thin films that benefit from efficient octupolar molecular packing are prepared for second harmonic generation (SHG) and electro-optic (EO) applications. The films are composed of 1,3,5-tricyano-2, 4,6-tris(p-diethylaminostyryl)benzene (TTB) in a ploymethylmetacrylate (PMMA) matrix on aluminum/BK7 glass (Al/BK7) and polyimide/indium tin oxide (PI/ITO) substrates. Octupolar films prepared on both substrates display polycrystalline and cylindrical domains. The molecular orientation, SHG efficiencies, and EO coefficients of the crystalline domains are measured. In the cylinders, the molecular crystal planes are oriented perpendicularly to the major cylinder axis, whereas in the polycrystals, the planes are randomly oriented. While both structures exhibit high and stable SHG and EO efficiencies, the cylinders, in particular, exhibited a very large SHG, a large EO coefficient, and high thermal stability; these characteristics will be useful in second order nonlinear optical applications.
    Advanced Functional Materials 02/2012; 22(4). · 10.44 Impact Factor

Publication Stats

1k Citations
552.22 Total Impact Points

Institutions

  • 1993–2014
    • Korea University
      • Department of Chemistry
      Sŏul, Seoul, South Korea
  • 2009–2013
    • Ajou University
      • Division of Energy Systems Research
      Seoul, Seoul, South Korea
    • Bharathiar University
      • Department of Chemistry
      Coimbatore, State of Tamil Nadu, India
  • 2011–2012
    • Yonsei University Hospital
      • Department of Internal Medicine
      Seoul, Seoul, South Korea
  • 1999–2008
    • Pukyong National University
      • Department of Chemistry
      Pusan, Busan, South Korea
  • 2006
    • Dankook University
      • Department of Chemistry
      Yŏng-dong, North Chungcheong, South Korea
  • 2002
    • Texas Tech University
      • Department of Chemistry and Biochemistry
      Lubbock, TX, United States
  • 2001
    • Kyung Hee University
      Sŏul, Seoul, South Korea