ABSTRACT: The study evaluated the pharmacokinetics of tramadol and its major metabolites O-desmethyltramadol (M1), N-desmethyltramadol (M2) and N-O didesmethyltramadol (M5) following a single oral administration of a sustained release (SR) 100mg tablet to dogs. Plasma tramadol concentration was greater than the limit of quantification (LOQ) in three dogs, M1 was quantified only in one dog while M2 and M5 were quantified in all of the dogs. The median values of C(max) (maximum plasma concentration), T(max) (time to maximum plasma concentration) and T(1/2) (half-life) for tramadol were 0.04 (0.17-0.02)mirog mL(-1), 3 (4-2) and 1.88 (2.211-1.435)h, respectively. M5 showed median values of C(max), T(max) and T(1/2) of 0.1 (0.19-0.09)microg mL(-1), 2 (3-1) and 4.230 (6.583-1.847)h, respectively. M2 showed median values of C(max), T(max) and T(1/2) of 0.22 (0.330-0.080)microg mL(-1), 4 (7-3) and 4.487 (6.395-1.563)h, respectively. The findings suggest that the SR formulation of tramadol may not have suitable pharmacokinetic characteristics to be administered once-a-day as an effective and safe treatment for pain in the dog.
The Veterinary Journal 03/2008; 180(2):253-5. · 2.24 Impact Factor
ABSTRACT: Total Antioxidant Potential (TAP) frequently better describes the antioxidant properties of the
complex biological samples (such as herbal extracts or blood plasma) than concentrations of all
individual antioxidants in the sample. In the paper improved fluorometric assays of the TAP
measurements have been described. These tests adopt thermolabile diazocompound [2,2’-diazobis
(2-amidinopropane) dihydrochloride – AAPH] generating peroxyl radical, Fenton reaction produced
hydroxyl radicals and SIN-1 (3-morpholino-sydnonimine) generating peroxynitrite. These
radicals oxidize the analyzed samples and so called „detector”. Sample competes with the reaction between detector and radical; therefore, it delays this reaction. Results are calculated from the delay time
during which antioxidants are consumed. Assays related to different radicals are described and compared.
A N N A L E S U N IV E R S I T A T I S MA R IA E C U R I E - S K Ł O D OWS K A L U B L IN – P O L O N IA Sectio DD. 01/2006; LXI, 6:55-59.
ABSTRACT: The lipophilicity (R(Mo)) and specific hydrophobic surface area of seven 1,2-benzisothiazol-3(2H)-ones have been determined by reversed-phase TLC and the effect of different mobile-phase modifiers (acetone, acetonitrile, methanol) on the retention has been studied. The linear correlations between the volume fraction of the organic solvent and the R(M) values over a limited range were established for each solute with high values of correlation coefficients (>0.99). The influence of solvent pH on R(M) values was investigated.
Journal of Chromatography 04/2002; 952(1-2):295-9. · 4.53 Impact Factor
Acta poloniae pharmaceutica 64(3):277-80. · 0.66 Impact Factor