Kiyoharu Itoh

Nagoya Municipal Industrial Research Institute, Nagoya, Aichi, Japan

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Publications (3)3.4 Total impact

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    ABSTRACT: The degradation of several azo dyes by cell-free extract from Aeromonas hydrophila var. 24B has been studied. The compounds 4-dimethylaminoazobenzene, 4-diethylaminoazobenzene, 2′-carboxy-4-dimethylaminoazo-benzene, 2′-carboxy-4-diethylaminoazobenzene, 4′-carboxy-4-dimethylaminoazobenzene, and 2′-methoxy-carbonyl-4-dimethylaminoazobenzene were appreciably degraded by the cell-free extract. Azobenzene and 4-carboxyazobenzene were not degraded. Aniline was detected by gas chromatography-mass spectrometry as a degradation product from 4-aminoazobenzene and 4-dimethylaminoazobenzene. 4-DimethyIaminoaniline was detected by gas chromatography-mass spectrometry and spectrophotofluorimetry as a degradation product from 2′-carboxy-4-dimethylaminoazobenzene. The cell-free extract had high substrate specificity for 2′-carboxy-4-dimethylaminoazobenzene.
    Review of Progress in Coloration and Related Topics 10/2008; 103(11):395 - 398. · 0.90 Impact Factor
  • Kiyoharu Itoh, Chizuko Yatome
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    ABSTRACT: The decolorization of six xanthene dyes (conc. 100 microM) by a white rot fungus, Coriolus versicolor (C. versicolor), was investigated in liquid culture. The decolorization of Fluorescein, 4-Aminofluorescein, and 5-Aminofluorescein by the fungus was 85.0, 95.0, and 91.9% after 14 days incubation, respectively. However, no decolorization of Rhodamine B, Rhodamine 123 hydrate, and Rhodamine 6G was observed. The first three dyes also were decolorized with cell-free extracts from C. versicolor. The decolorization activity was 10.2, 6.7, and 7.2 microM min(-1)mg(-1), respectively. Thin layer chromatography (TLC) analyses indicated that degradation of Fluorescein was occurring with the detection of three degradation products.
    Journal of Environmental Science and Health Part A 02/2004; 39(9):2383-9. · 1.25 Impact Factor
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    ABSTRACT: The decolorization of methyl red directed toward rapid reduction of azo group by a mixed culture of Bacillus sp. and Pseudomonas stutzeri was investigated. The improvement of the decolorization of methyl red was obtained by a mixed culture (a mixed culture 2) with particular proportion of two bacteria (Bacillus sp. and Pseudomonas stutzeri). The mixed culture 2 was grown in a LB medium with methyl red. The improvement of the decolorization of methyl red was obtained only by the growing mixed culture and not obtained by the mixture of the resting cells.
    Journal of Environmental Science and Health Part A 04/2002; 37(3):415-21. · 1.25 Impact Factor