-
[show abstract]
[hide abstract]
ABSTRACT: The title compound glaucocalyxin A (1) (7α, 14β-dihydroxy-ent-kaur-16-en-3,15-dione) isolated from the leaves of isodon excisoides was characterized by IR, 1H NMR, 13C NMR, 1H-1H COSY, HMQC, HMBC, and EIMS, and its crystal structure was determined by single-crystal X-ray diffraction. The X-ray crystal structure revealed that the molecular backbone of the chosen crystal is a tetracyclic system, including three six-membered rings and a five-membered ring, and the three six-membered rings are in a chair-like conformation. The five-membered ring adopts a twisted envelope-like conformation, and its geometrical parameters were compared with theoretical calculations at the B3LYP and HF level of theory. The molecules form extensive networks through the intra- and intermolecular hydrogen bonds. The experimental NMR data were interpreted with the aid of magnetic shielding constant calculations, by means of the GIAO (gauge-lncluding atomic orbitals) method. Calculated and experimental results were compared with a satisfactory level of agreement. Molecular electrostatic potential map was used in an attempt to identify key features of the diterpenoid glaucocalyxin A that is necessary for its activity. Calculations of molecular electrostatic potential and stabilization energies suggest that the protonation of glaucocalyxin A will be able to occur on carbonyl oxygen atoms.
Chinese journal of chemical physics 07/2009; 22(3):275. · 0.69 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the chemical constituents of Ligularia xanthotricha.
Silica gel column chromatography and preparative TLC were employed for the isolation and purification. The structures were identified on the basis of spectral data (IR, EI-MS, 1H-NMR, 13C-NMR, DEPT) and chemical evidence.
Seven compounds were isolated and identified as follows: lupeol (1), lupeol palmitate (2), 3, 28-dihydroxyl-lupeol (3), betulinic acid (4), taraxasterol (5), taraxasteryl palmitat (6) and taraxasteryl acetate(7).
All the compounds were isolated from this plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 03/2008; 33(3):272-5.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents of Ligularia intermedia of Shanxi.
The compounds were isolated by column chromatography on silica gel and preparative TLC. The structures were identified by IR, MS, 1D/2DNMR spectral data and X-ray single crystal diffraction and other methods1.
Nine compound were isolated and identified as 8beta-hydroxyeremophil-7(11)-ene-12, 8alpha(4beta, 6alpha)-diolide (1), 8beta-methoxyeremophil-7(11)-ene-12, 8alpha(4beta, 6alpha)-diolide (2), petasin (3), isopetasin (4), liguhodgsonal (5), ligudentatol (6), ligujapone (7), lupeol (8) and lupeol palmitate (9).
Compounds 2, 3, 4, 6, 7 and 9 were isolated from the plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2007; 32(11):1044-7.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents of herbs of Artemisia ordosica.
The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physicochemical properties.
Eight flavones were obtained and they were identified as isosakuranetin, 7, 4'-dimethylaro madendrin, acacetin, cirsimaritin, rhamnetin, eupatolin, 5, 7, 2', 4'-tetrahydroxy-6, 5'-dimethoxyflavone, hyperoside.
All the compounds were obtained from herbs of A. ordosica for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 01/2007; 31(23):1959-61.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the chemical constituents of whole herbs of Seriphidium terrae-albae.
Various chromatographic techniques were employed for the isolation and purification of the chemical constituents. The structures were elucidated by chemical and spectral analysis.
Seven flavonoid compounds were isolated and identified as apigenin, eupatilin, chrysoeriol, quercetin-3, 3'-dimethyl ether, quercetagetin-3, 6-dimethyl ether, quercetagetin-3, 6-dimethyl ether-7-O-beta-D-pyranoglucoside and quercetagetin-3, 6-dimethyl ether-4'-O-beta-D-pyranoglucoside, respectively.
All of these flavonoid compounds were isolated from the plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 06/2006; 31(10):820-2.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constitutes in Rabdosia japonica var. galaucocalyx .
The compounds were isolated by nomal phase silica gel chromatography. The structures were identified by physical and spectral data.
Nine triterpenoids were isolated and identified as friedelin (I), 3beta, 28-dihydroxy-ursane (II), ursolic acid (III), 3beta-acetyloxy-ursolic acid (IV), 2alpha, 3alpha-dihydroxy-urs-12-en-28-oic acid (V), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid (VI), oleanic acid (VII), beta-sitosterol (VIII), daucousterol (IX).
Compounds I, II, IV, V and VI were isolated from R. japonica var. galaucocalyx for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 01/2006; 30(24):1929-32.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the alkaloid constituents of Corydalis adunca.
The constituents were isolated on silica gel column and their structures were elucidated by IR, NMR, MS data.
Four alkaloid compounds were isolated from alcohol extract of the herb of C. adunca, and identified as tetrahydropalmatine, 1, 2, 3, 4-tetrahydro-6-methoxy-1-(4'-methoxyphenyl) methyl-7-isoquinolinol, coptisine and palmatine.
Four compounds were isolated from C. adunca for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 12/2005; 30(22):1756-7.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents of Artemisia ordosica.
The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties.
Eight flavonoids were obtained and identified as 5-hydroxy-7, 4'-dimethoxyflavanone, 5-hydroxy-7, 4'-dimethoxyflavone, 5, 4'-dihydroxy-7-methoxyflavone, 5, 7-dihydroxy-6, 4'-dimethoxyflavone, 5, 4'-dihydroxy-7-methoxyflavanone, 5, 3', 4'-trihydroxy-7-methoxyflavanone, 5, 7-dihydroxy-3', 4'-dimethoxyflavone and 5, 3', 4'-trihydroxy-7-methoxyflavone.
All the compounds were obtained from A. ordosica for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 10/2005; 30(18):1430-2.
-
[show abstract]
[hide abstract]
ABSTRACT: Ion solvation and ion association in solutions of NaBF4/DMF were studied by infrared and Raman spectroscopy and ab initio quantum chemistry. It was found that the sodium cation interacts on oxygen atom of C=O group in DMF molecules. The solvation number of Na+, four, was obtained according to the change analysis of CH3 rocking band. The splitting of the nu1 mode of BF4(-) anion indicates the presence of contact ion pairs. Ionic association degree increases with increasing molality of the salt. The solvated structure of Na+ and the geometry of Li+ BF4(-) ion pair were also suggested based on the ab initio calculations.
Guang pu xue yu guang pu fen xi = Guang pu 05/2005; 25(4):548-52. · 0.84 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents of Chondrilla piptocoma.
The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties.
Seven compounds were obtained and they are identified as luteolin, 5,7,4'-trihydroxy-3'-methoxy flavone, luteolin-7-O-beta-D-glucoside, apigenin, beta-sitosterol, stigmasterol, ursolic acid.
All the compounds were obtained from C. piptocoma for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 05/2005; 30(8):588-90.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the alkaloid constituents of Corydalis adunca.
The constituents were isolated on silica gel column and their structures were elucidated by IR, NMR, MS data.
Eight alkaloid compounds were isolated from alcohol extracts of the herb of C. adunca, and identified as dihydrosanguinarine (I), tetrahydrocolumbamine (II), 1,2,3,4-tetrahydro-7-methoxy-1-[(4-methoxy)phenyl]methyl-8-quinolinol (III), protopine (IV) and 6-acetonyl-5,6-dihydrosanguinarine (V).
Five compounds were isolated from C. adunca for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 03/2005; 30(3):195-7.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents from Lamium maculatum var. kansuense.
The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties.
Six compounds were obtained and identified as polypodine B (I), 5-OH-8-epiloganin (II), shlanzhiside methyl ester (III), liriodendrin (IV), quercitroside (V), uridine (VI).
Compound IV was found from genus Lamium for the first time and the rest of the compounds were found from Lamium maculatum var kansuense for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 03/2005; 30(4):272-4.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents from Eremosparton songoricum.
The compounds were isolated with silica gel column chromatography and the structures of these compounds were elucidated by means of spectral analysis.
The seven compounds were identified as: 5,7,4'-trihydroxyflavone (apigenin) (I), 5-hydroxy-7,4'-dimethoxyflavone (II), 5,7-dihydroxy-3',4'-dimethoxyflavone (III), 5,7-dihydroxy-4'-methoxyflavone (acacetin) (IV), 5,7,4'-trihydroxy-3'-methoxyflavone (chrysoeriol) (V), 5,6,3',4'-tetrahydroxy-7-methoxyflavone (pedalitin) (VI) and 5,4'-dihydroxy-7,3'-dimethoxyflavone-4'-O-D-glucoside (flavogadorinin) (VII).
These constituents were obtained from E. songoricum for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 02/2005; 30(2):126-8.
-
[show abstract]
[hide abstract]
ABSTRACT: The isomeric distribution of conjugated linoleic acids (CLA) in the tissue lipids of hens in relation to that in the diet was examined. Silver-ion high-performance liquid chromatography was used to quantify individual CLA isomers in total tissue lipids, phospholipids, and triacylglycerols. It was found that the deposition of CLA isomers in hen tissues was selective. All tissues including serum, liver, heart, kidney, abdominal fat, and leg and breast muscles had lesser amounts of total cis/trans isomers ranging from 75.87 to 89.13% of total CLA, which was in contrast to the value of 92% of total CLA in the dietary lipids. Total trans/trans isomers in all tissue lipids ranging from 6.11 to 18.02% of total CLA were greater than that in the diet (4.19%). Among the individual trans/trans isomers, all tissues except for adipose tissue and brain incorporated greater amounts of t-12,t-14-18:2, t-11,t-13-18:2,t-10,t-12-18:2, t-9,t-11-18:2, and t-18,t-10-18:2 compared with the values of the diet. Within the cis/trans group, lesser amounts of c-10,t-12/t-10,c-12-18:2 were found to incorporate into all tissues compared with the value of the diet. Serum and liver had higher percentages of c-9,t-11/t-9,c-11, whereas the other tissues had similar levels of this isomer compared with that of the diet. It was also observed that supplementation of CLA in the diet of layer hens decreased the concentration of docosahexaenoic acid (22:6n-3) in all of the tissue lipids. It is concluded that dietary CLA can transfer to the tissue but that incorporation of CLA isomers into the tissue is selective in hens.
Journal of Agricultural and Food Chemistry 10/2003; 51(19):5654-60. · 2.82 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents from the water-soluble part of Euphrasia regelii.
The compounds were isolated by macroporous resin and column chromatography, and identified by IR, NMR data.
Two special compounds were isolated and identified.
Two compounds were isolated from this plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 09/2003; 28(8):733-4.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the chemical constituents from Lamium maculatum L. var Kansuense.
The chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physico-chemical properties.
Ten compounds were obtained and they were identified as D-mannitol, beta-sitosterol, stigmasterol, rutin, 3'-methylquercetin-3-O-rutinoside, n-butyl-beta-D-fructopyranoside, daucosterol, acteoside, 20-hydroxyecdysone, allantoin.
All the compounds were obtained from L. maculatum L. var Kansuense for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 09/2003; 28(8):730-2.
-
[show abstract]
[hide abstract]
ABSTRACT: To provide the foundation for reasonable utilization by analyzing the essential oils of Ligularia virgaurea.
The essential oils were extracted by using steam distillation and separated with GC capillary columns. The components were quantitatively determined with normalization method, and were identified with GC-MS.
41 components were identified, which took up 72.73% of the essential oils.
The main components of essential oils were 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol(14.369%), crotonic acid, 2,2-dimethyl-butanoic acid, 1-methyl-3-(1-methylethyl)-benzene, (1s-endo)-1,7,7-trimethyl-bicyclo[2,2,1]heptan-2-ol, trans-1-methyl-4-(1-methylethyl)-2-cyclohexen-1-ol, alpha-cadinol and alpha,alpha,4-trimethyl-3-cyclohexene-1-methanol.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 08/2003; 28(7):627-9.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the chemical constituents of Anaphalis sinica.
Various chromatographic techniques were employed for the isolation and purification of the constituents. The structures were elucidated by chemical and spectral analysis.
Six flavonoid compounds were isolated and identified as kaempferol, tiliroside, quercetin, quercetin -3-O-beta-D-glucosides, scutellarin, 5,7-dihydroxy-8-methoxyflavone and one pyrone were isolated and identified as helipyrone.
All of these compounds was isolated from the plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2003; 28(6):530-3.
-
[show abstract]
[hide abstract]
ABSTRACT: Production of conjugated linoleic acids (CLA) using castor oil as starting material involves conversion of ricinoleic acid to methyl 12-mesyloxy-octadec-9-enoate (MMOE) followed by dehydration. This process usually uses 1,8-diazabicyclo-(5.4.0)-undec-7-ene (DBU) as an expensive dehydrating reagent. The present study reports that potassium hydroxide (KOH) can serve as a dehydrating reagent in replacement of DBU. The results showed that conversion of MMOE to CLA catalyzed by KOH was an efficient reaction, with a 77% conversion efficiency at 80 degrees C. The CLA isomeric profile produced in KOH-catalyzed dehydration reaction was similar to that catalyzed by DBU. The CLA mixture produced in KOH-catalyzed dehydration of MMOE at 80 degrees C contained 72% 9c,11t-18:2 and 26% 9c,11c-18:2 while in that catalyzed by DBU, 9c,11t-18:2 and 9c,11c-18:2 accounted for 78 and 16%, respectively. It was found that the temperature of dehydration was an important factor in the determination of CLA isomer composition and yield of conversion. Elevating the temperature from 78 to 180 degrees C decreased not only the conversion efficiency but also production of total c,t-18:2 and c,c-18:2 isomers regardless of dehydration catalyzed by either DBU or KOH. It is concluded that KOH may replace DBU as a dehydrating reagent in conversion of MMOE to CLA when the reaction conditions are optimized.
Chemistry and Physics of Lipids 11/2002; 119(1-2):23-31. · 2.57 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The present study was designed to compare the conjugated linoleic acid (CLA) isomeric distribution pattern in the liver of suckling rats in relation to those in the milk and maternal diet. Silver-ion HPLC was used to separate individual CLA isomers. It was found that the isomeric distribution pattern in the milk was very similar to that in the maternal dietary fat. However, the CLA isomeric distribution patterns in the liver phospholipids (PL) and triacylglycerols were different from those in the diet and milk. In the liver PL, total cisltrans isomers accounted for 63.6-63.9% of total CLA, which was in contrast to the values of 88.1-89.1% in the milk and diet. In the liver PL, total transltrans isomers were 20.6-20.8% of the total CLA isomers whereas they were only 2.6-3.7% in the milk and diet. It is concluded that trans/trans-CLA were preferentially incorporated into the liver whereas for the incorporation of cis/trans-CLA there was partial discrimination.
British Journal Of Nutrition 04/2002; 87(3):253-60. · 3.01 Impact Factor
