Yan-Xiu Zhao

Shandong Normal University, Chi-nan-shih, Shandong Sheng, China

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Publications (9)9.31 Total impact

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    ABSTRACT: Transgenic rice plants co-expressing the Suaeda salsa SsNHX1 (vacuolar membrane Na+/H+ antiporter) and Arabidopsis AVP1 (vacuolar H+-PPase) showed enhanced salt tolerance during 3d of 300mM NaCl treatment under outdoor growth conditions. These transgenic rice seedlings also grew better on MS medium containing 150mM NaCl compared to SsNHX1-transformed lines and non-transformed controls. Measurements on isolated vacuolar membrane vesicles derived from the salt stressed SsNHX1+AVP1-transgenic plants demonstrated that the vesicles had increased V-PPase hydrolytic activity in comparison with the Ss-transgenics and non-transgenics. Moreover the V-PPase activity was closely related to the development period of the SA-transgenic seedlings and markedly higher in 3-week-old seedlings than in 5-week-old seedlings. Statistic analysis indicated that the SA-transgenic rice plants contained relatively more ions with higher K+/Na+ ratio in their shoots compared to the SsNHX1-transformed lines upon salt treatment. Furthermore, these SA-transformants also exhibited relatively higher level of photosynthesis and root proton exportation capacity whereas reduced H2O2 generation in the same plants. In general, these results supported the hypothesis that simultaneous expression of the SsNHX1 and AVP1 conferred greater performance to the transgenic plants than that of the single SsNHX1.
    Molecular Breeding 06/2006; 17(4):341-353. · 3.25 Impact Factor
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    ABSTRACT: The GST (glutathione S-transferase) and GST+CAT1 (catalase 1) of Suaeda salsa were introduced into a low temperature-sensitive rice cultivar (Oryza sativa cv. Zhonghua No.11) by Agrobacterium tumefaciens-mediated transformation under the control of cauliflower mosaic virus (CaMV) 35S promoter, and the transformed calli and plantlets were screened on Murashige and Skoog (1962) medium supplemented with hygromycin 25 microg/mL and cefotaxime 300 microg/mL. The putative primary transformants (T(0) generation) were acclimatized at 26 degrees C /22 degrees C in a greenhouse for 7 d, and then transplanted to the field, where they grew up to maturity under outdoor conditions. 25 and 14 independent transgenic lines of T(1) generation carrying the GST and GST+CAT1 genes, respectively, were identified by PCR amplification. Transgene expression was monitored by RNA-blot hybridization using total RNA samples from leaf tissues. To investigate whether expressing the Suaeda salsa GST and GST+CAT1 in transgenic rice increased low temperature stress tolerance, the T(4) 14-day-old transgenic and non-transgenic rice seedlings were transferred to a low temperature (day 7 degrees C/night 4 degrees C) growth chamber for 3-6 d. The experimental data showed that expressing the Suaeda salsa GST and GST+CAT1 enhanced low temperature stress resistance in transgenic rice seedlings. When treated with low temperature, both GST and CAT activity increased in the transformants with the time of temperature treatment. These transgenic rice plant seedlings exhibited a higher level of photosynthetic capacity than those of the non-transgenic control seedlings under low temperature treatment. Whereas, there were lower H(2)O(2) and MDA (malondialdehyde) content, and relative electrolyte leakage through the plasma membrane was also lower in transgenic rice seedlings than in the parent line under low temperature condition. The results also indicated that GST+CAT1 co-expression conferred greater level of low temperature stress tolerance to the transformed rice plants compared to the single GST transformed plants.
    Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology 05/2006; 32(2):231-8.
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    ABSTRACT: A full-length cDNA of SsCCD1 gene encoding carotenoid cleavage dioxygenase was isolated from a halophyte Suaeda salsa. Sequence analysis revealed that SsCCD1 shares a high homology with AtCCD1 from Arabidopsis thaliana. The 1972bp full-length SsCCD1 has an open reading frame of 1677bp and encodes a 62.5KDa protein, with 5′- and 3′-untranslate region of 72bp and 229bp, respectively. Northern blotting analysis indicated that the SsCCD1 mRNA levels were significantly up-regulated by NaCl and drought stresses in both root and aerial part tissues. High temperature and ABA also increased the transcriptional level of SsCCD1. Transgenic arabidopsis with overexpression of SsCCD1 gene did not show an increased endogenous ABA concentration, but decreased the carotenoid concentration, indicating that CCD enzyme may be involved in the carotenoid catabolism.
    Plant Growth Regulation 04/2005; 46(1):61-67. · 1.67 Impact Factor
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    ABSTRACT: A novel spectrofluorimetric method using vanillin-8-aminoquinoline (VAQ) as fluorescent probe was developed for the determination of superoxide anion radical (O2−). The new fluorescent probe was characterized by elemental analysis and IR spectra. Under the optimum conditions of the determination, the linear calibration range and the detection limit of the developed method for superoxide anion radical were in the range (0.0–1.0)×10−5 and 2.0×10−8 mol l−1, respectively. The effect of interferences was studied. The proposed method was applied to determine the generation rate of superoxide anion radical in the course of aging in red sage successfully.
    Analytica Chimica Acta 01/2004; · 4.39 Impact Factor
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    ABSTRACT: Accumulation and analysis of expressed sequence tags (ESTs) data from halophytic plant is a relatively rapid and cheap way for discovering new genes related to salinity tolerance. We constructed a NaCl-treated cDNA library of Thellungiella halophila and sequenced more than 1500 randomly selected clones. By sequence analysis, 813 unique clones were identified: 549 showed homology to previously identified genes, 264 matched uncharacterized genes. All our EST data are available on the Internet. The identity between T. halophila and Arabidopsis thaliana cDNA sequences in our EST collection are 95.76% in total ESTs and 95.36% in non-redundant clones. At least eight classes of genes were related to the salt-tolerance, which accounted for about 18.89% of total sequenced ESTs. T. halophila that has many features similar to Arabidopsis can be adopted as a halophytic model for stress-tolerance research.
    Plant Science. 01/2004;
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    ABSTRACT: Wheat productivity is severely affected by soil salinity mainly due to Na+ toxicity to plant cells. To improve the yield performance of wheat in saline soils, we have generated transgenic wheat expressing a vacuolar Na+/H+ antiporter gene AtNHX1 from Arabidopsis thaliana, to enhance the plant capacity of reducing cytosolic Na+ by sequestering Na+ in the vacuole. The AtNHX1 transgenic wheat lines exhibited improved biomass production at the vegetative growth stage and germination rates in severe saline conditions. A field trial revealed that the transgenic wheat lines produced higher grain yields and heavier and larger grains in the field of saline soils with the electrical conductivity values of soil saturation extracts (ECe) of 10.6 and 13.7 dS m−1. The transgenic lines accumulated a lower level of Na+ and a higher level of K+ in the leaves than non-transgenic plants under saline conditions (100 and 150 mM NaCl). These results indicate that the salt tolerance of wheat and grain yield in saline soils can be improved by enhancing the level of the vacuolar Na+/H+ antiporter.
    Plant Science. 01/2004;
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    ABSTRACT: Peroxiredoxin Q, as a member of peroxiredoxin gene family, was most recently cloned from plants. However, its molecular characteristics remain unclear. In this paper, we cloned a peroxiredoxin Q gene from a 400 mM NaCl-treated cDNA library of Suaeda salsa. The SsPrx Q gene (1 003 bp) contains an open reading frame of 214 amino acids with a molecular mass of 23.6 kDa. SsPrx Q showed high homology to the Prx Q of Sedum lineare, with 76% identities in deduced amino acid sequence. Southern blot analysis indicated that SsPrx Q gene has a single copy in S. salsa genome. Northern blot analysis showed that SsPrx Q gene expression was up-regulated by NaCl, mannitol, low temperature, H2O2, methyl viologen, and ABA. Studies of the recombinant protein produced in E. coli cells exhibited that it actually had a thioredoxin-dependent peroxidase activity, the characteristic of the Prx family. These results indicated that SsPrx Q may play an important role in protecting S. salsa from environmental stresses.
    Plant Science. 01/2004;
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    ABSTRACT: Chloroplast is a kind of special cell organ in plant cells.Since 1988,Scientists have realized its advantages in plant gene engineering.It's high efficient expression and safety made it been attached more and more importance to. This paper introduces the chloroplast transformation,including its advantages,methods,uses and defects.
    Hereditas (Beijing) 08/2003; 25(4):495-8.
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    ABSTRACT: Na+/H+ antiporter plays an important role in mechanisms of the plant salt tolerance, it extrudes Na+ from cell energized by the proton gradient generated by the plasm membrane H(+)-ATPase and/or compartmentalizes Na+ in vacuole energized by the proton gradient generated by the vacuolar membrane H(+)-ATPase and H(+)-PPiase. This review mainly discusses the latest progress in the study of Na+/H+ antiporter in plant and yeast at molecular level.
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology 02/2002; 18(1):16-9.