A. Goldsmith

Publications (2)18.41 Total impact

• Source

  Available from: ncbi.nlm.nih.gov

  Article: Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core.

  J M Diprose, J N Burroughs, G C Sutton, A Goldsmith, P Gouet, R Malby, I Overton, S Ziéntara, P P Mertens, D I Stuart, J M Grimes
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  ABSTRACT: The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg(2+) ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.
  The EMBO Journal 01/2002; 20(24):7229-39. · 9.20 Impact Factor
• Article: Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core

  J.M. Diprose, J.N. Burroughs, G.C. Sutton, A. Goldsmith, P. Gouet, R. Malby, I. Overton, S. Zi|[eacute]|ntara, P.P.C. Mertens, D.I. Stuart, J.M. Grimes
  [show abstract] [hide abstract]
  ABSTRACT: The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg2+ ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.
  The EMBO Journal 12/2001; 20(24):7229-7239. · 9.20 Impact Factor