ABSTRACT: To determine whether vaccine site-associated sarcomas (VSS) from cats contain polyomavirus antigen or DNA.
50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats.
Sections from each tissue block were evaluated for polyomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-murine polyomavirus polyclonal antiserum as the primary antibody. The DNA was extracted from sections of each tissue block, and a polymerase chain reaction assay was performed, using primers designed to amplify regions of the bovine polyomavirus genome and consensus polyomavirus primers designed to detect unknown polyomaviruses.
Polyomavirus antigen and DNA were not detected in any of the VSS.
Results suggest that polyomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.
American Journal of Veterinary Research 07/2001; 62(6):828-32. · 1.27 Impact Factor