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ABSTRACT: Boundary lubrication is characterized by sliding surfaces separated by a molecularly thin film that reduces friction and wear of the underlying substrate when fluid lubrication cannot be established. In this study, the wear and replenishment rates of articular cartilage were examined in the context of friction coefficient changes, protein loss, and direct imaging of the surface ultrastructure, to determine the efficiency of the boundary lubricant (BL) layer. Depletion of cartilage lubricity occurred with the concomitant loss of surface proteoglycans. Restoration of lubrication by incubation with synovial fluid was much faster than incubation with culture media and isolated superficial zone protein. The replenishment action of the BL layer in articular cartilage was rapid, with the rate of formation exceeding the rate of depletion of the BL layer to effectively protect the tissue from mechanical wear. The obtained results indicate that boundary lubrication in articular cartilage depends in part on a sacrificial layer mechanism. The present study provides insight into the natural mechanisms that minimize wear and resist tissue degeneration over the lifetime of an organism.
Journal of biomechanics 08/2012; 45(14):2426-31. · 2.66 Impact Factor
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ABSTRACT: Mechanostasis describes a complex and dynamic process where cells maintain equilibrium in response to mechanical forces. Normal physiological loading modes and magnitudes contribute to cell proliferation, tissue growth, differentiation and development. However, cell responses to abnormal forces include compensatory apoptotic mechanisms that may contribute to the development of tissue disease and pathological conditions. Mechanotransduction mechanisms tightly regulate the cell response through discrete signaling pathways. Here, we provide an overview of links between pro- and anti-apoptotic signaling and mechanotransduction signaling pathways, and identify potential clinical applications for treatments of disease by exploiting mechanically-linked apoptotic pathways.
Progress in Biophysics and Molecular Biology 08/2011; 106(3):517-24. · 3.20 Impact Factor
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ABSTRACT: Friction and adhesion of articular cartilage from high- and low-load-bearing regions of bovine knee joints were examined with a tribometer under various loads and equilibration times. The effect of trapped lubricants was investigated by briefly unloading the cartilage sample before friction testing, to allow fluid to reflow into the contact interface and boundary lubricants to rearrange. Friction and adhesion of high-load-bearing joint regions were consistently lower than those of low-load-bearing regions. This investigation is the first to demonstrate the regional variation in the friction and adhesion properties of articular cartilage. Friction coefficient decreased with increasing contact pressure and decreasing equilibration time. Briefly unloading cartilage before the onset of sliding resulted in significantly lower friction and adhesion and a loss of the friction dependence on contact pressure, suggesting an enhancement of the cartilage tribological properties by trapped lubricants. The results of this study reveal significant differences in the friction and adhesion properties between high- and low-load-bearing joint regions and elucidate the role of trapped lubricants in cartilage tribology.
Journal of biomechanics 06/2011; 44(11):2015-20. · 2.66 Impact Factor
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ABSTRACT: Articular cartilage is the load bearing soft tissue that covers the contacting surfaces of long bones in articulating joints. Healthy cartilage allows for smooth joint motion, while damaged cartilage prohibits normal function in debilitating joint diseases such as osteoarthritis. Knowledge of cartilage mechanical function through the progression of osteoarthritis, and in response to innovative regeneration treatments, requires a comprehensive understanding of the molecular nature of interacting extracellular matrix constituents and interstitial fluid. The objectives of this study were therefore to (1) examine the timescale of cartilage stress-relaxation using different mechanistic models and (2) develop and apply a novel (termed "sticky") polymer mechanics model to cartilage stress-relaxation based on temporary binding of constituent macromolecules. Using data from calf cartilage samples, we found that different models captured distinct timescales of cartilage stress-relaxation: monodisperse polymer reptation best described the first second of relaxation, sticky polymer mechanics best described data from ∼1-100 seconds of relaxation, and a model of inviscid fluid flow through a porous elastic matrix best described data from 100 seconds to equilibrium. Further support for the sticky polymer model was observed using experimental data where cartilage stress-relaxation was measured in either low or high salt concentration. These data suggest that a complete understanding of cartilage mechanics, especially in the short time scales immediately following loading, requires appreciation of both fluid flow and the polymeric behavior of the extracellular matrix.
Materials Science and Engineering C 05/2011; 31(4):781-788. · 2.69 Impact Factor
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ABSTRACT: The purpose of this study was to evaluate and compare the resulting knee kinematics and stability of an anatomic superficial MCL (sMCL) reconstruction and a non-anatomic sMCL reconstruction.
In a cadaveric model, normal knee stability and kinematics were compared with sMCL deficient knees and with two experimental sMCL reconstructions. The first reconstruction (AnatRecon) attempted to anatomically reconstruct the sMCL. The second reconstruction (ShortRecon) used a shorter graft to mimic the effect of failing to reproduce the anatomic length of the sMCL. Changes in position of the femur with respect to the tibia were measured with an electromagnetic tracking system during simulated active knee extension and during passive knee stability testing in the sMCL intact knee, the sMCL deficient knee, and the two experimental reconstructions.
Simulated active knee extension demonstrated a significant increase in external tibial rotation of ShortRecon compared to AnatRecon between 30° and 80° of knee flexion (mean difference <3.0° over the range of knee flexion angles; P < 0.008), and a significant increase in external tibial rotation of ShortRecon compared to the intact sMCL was found at 60° and 70° of knee flexion (mean difference <2.0°over the range of knee flexion angles; P < 0.008). Passive joint stability testing demonstrated that division of the sMCL produced approximately 6° of valgus laxity at 30° of knee flexion and increased external tibial rotation of approximately 5° at 30°, 9° at 60°, and 10° at 90° of knee flexion, respectively. AnatRecon restored normal knee kinematics and stability. Additionally, passive stability testing demonstrated a significant increase in external tibial rotation of ShortRecon compared to AnatRecon at 60° (mean difference = 3.7°; P < 0.05) and 90° of knee flexion (mean difference = 4.9°; P < 0.05).
Anatomic reconstruction of the sMCL effectively restored knee kinematics and stability in the sMCL deficient knee. Altering the normal ligament length resulted in measurable changes in knee kinematics and stability. This study suggests that in cases of chronic valgus knee instability, anatomic sMCL reconstruction would provide better results than non-anatomic sMCL reconstruction.
Knee Surgery Sports Traumatology Arthroscopy 04/2011; 19 Suppl 1:S60-8. · 2.21 Impact Factor
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ABSTRACT: Boundary lubrication of articular cartilage by conformal, molecularly thin films reduces friction and adhesion between asperities at the cartilage-cartilage contact interface when the contact conditions are not conducive to fluid film lubrication. In this study, the nanoscale friction and adhesion properties of articular cartilage from typical load-bearing and non-load-bearing joint regions were studied in the boundary lubrication regime under a range of physiological contact pressures using an atomic force microscope (AFM). Adhesion of load-bearing cartilage was found to be much lower than that of non-load-bearing cartilage. In addition, load-bearing cartilage demonstrated steady and low friction coefficient through the entire load range examined, whereas non-load-bearing cartilage showed higher friction coefficient that decreased nonlinearly with increasing normal load. AFM imaging and roughness calculations indicated that the above trends in the nanotribological properties of cartilage are not due to topographical (roughness) differences. However, immunohistochemistry revealed consistently higher surface concentration of boundary lubricant at load-bearing joint regions. The results of this study suggest that under contact conditions leading to joint starvation from fluid lubrication, the higher content of boundary lubricant at load-bearing cartilage sites preserves synovial joint function by minimizing adhesion and wear at asperity microcontacts, which are precursors for tissue degeneration.
Journal of biomechanics 02/2011; 44(7):1340-5. · 2.66 Impact Factor
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ABSTRACT: In developing countries, tibial shaft fractures are frequently stabilised using Surgical Implant Generation Network (SIGN) nails. Despite widespread use throughout the world, little is known regarding their biomechanical properties. This study aimed to compare the mechanical stiffness of the SIGN tibial nail with a standard hollow tibial nail.
A fracture gap model was created to simulate a comminuted mid-shaft tibia fracture (AO/OTA42-C3) using synthetic composite bones. The constructs were stabilised with either a 9 mm solid SIGN nail or a 10 mm hollow Russell-Taylor nail. Both nail systems were interlocked proximally and distally. Following fixation, the specimens were loaded in axial, torsional, and cyclical axial modes to calculate construct stiffness and irreversible (plastic) deformation.
The mean axial stiffness for the SIGN nail constructs was 47% higher than mean stiffness for the RT nail constructs (p<0.001). The difference in torsional stiffness was not statistically significant. However, the SIGN group demonstrated 159% more irreversible deformation than the Russell-Taylor group (p=0.006) for the loading parameters studied.
The SIGN tibial nail, despite its slightly smaller diameter, can provide similar construct stiffness and stability, when compared to a larger hollow nail for stabilisation of tibial shaft fractures.
Injury 07/2010; 41(7):753-7. · 1.98 Impact Factor
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ABSTRACT: To determine the roles of superficial zone protein (SZP), hyaluronan (HA), and surface-active phospholipids (SAPL) in boundary lubrication of articular cartilage through systematic enzyme digestion using trypsin, hyaluronidase, and phospolipase-C (PLC) surface treatments.
The friction coefficient of articular cartilage surfaces was measured with an atomic force microscope (AFM) before and after enzyme digestion. Surface roughness, adhesion, and stiffness of the articular surface were also measured to determine the mechanism of friction in the boundary lubrication regime. Histology and transmission electron microscopy were used to visualize the surface changes of treatment groups that showed significant friction changes after enzyme digestion.
A significant increase in the friction coefficient of both load-bearing and non load-bearing regions of the joint was observed after proteolysis by trypsin. Treatment with trypsin, hyaluronidase, or PLC did not affect the surface roughness. However, trypsin treatment decreased the adhesion significantly. Results indicate that the protein component at the articular cartilage surface is the main boundary lubricant, with SZP being a primary candidate. The prevailing nanoscale deformation processes are likely plastic and/or viscoelastic in nature, suggesting that plowing is the dominant friction mechanism.
The findings of this study indicate that SZP plays an intrinsic and critical role in boundary lubrication at the articular surface of cartilage, whereas the effects of HA and SAPL on the tribological behavior are marginal.
Osteoarthritis and Cartilage 07/2010; 18(7):956-63. · 3.90 Impact Factor
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ABSTRACT: To quantify the concentration of superficial zone protein (SZP) in the articular cartilage and synovial fluid of patients with advanced osteoarthritis (OA) and to further correlate the SZP content with the friction coefficient, OA severity, and levels of proinflammatory cytokines.
Samples of articular cartilage and synovial fluid were obtained from patients undergoing elective total knee replacement surgery. Additional normal samples were obtained from donated body program and tissue bank sources. Regional SZP expression in cartilage obtained from the femoral condyles was quantified by enzyme-linked immunosorbent assay (ELISA) and visualized by immunohistochemistry. Friction coefficient measurements of cartilage plugs slid in the boundary lubrication system were obtained. OA severity was graded using histochemical analyses. The concentrations of SZP and proinflammatory cytokines in synovial fluid were determined by ELISA.
A pattern of SZP localization in knee cartilage was identified, with load-bearing regions exhibiting high SZP expression. SZP expression patterns were correlated with friction coefficient and OA severity; however, SZP expression was observed in all samples at the articular surface, regardless of OA severity. SZP expression and aspirate volume of synovial fluid were higher in OA patients than in normal controls. Expression of cytokines was elevated in the synovial fluid of some patients.
Our findings indicate a mechanochemical coupling in which physical forces regulate OA severity and joint lubrication. The findings of this study also suggest that SZP may be ineffective in reducing joint friction in the boundary lubrication mode at an advanced stage of OA, where other mechanisms may dominate the observed tribological behavior.
Arthritis & Rheumatism 05/2010; 62(9):2680-7. · 7.87 Impact Factor
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ABSTRACT: It is important to determine the three-dimensional nonuniform deformation of articular cartilage in its native environment. A new magnetic resonance imaging (MRI)-based technique (cartilage deformation by tag registration (CDTR)) has been developed, which can determine such deformations provided that the compressive load-displacement response of the knee reaches a quasi-steady state during cyclic loading. The objectives of this study were (1) to design and construct an apparatus to cyclically compress human cadaveric knees to physiological loads in a MRI scanner, (2) to determine the number of load cycles required to reach a quasi-steady-state load-displacement response for cyclic loading of human cadaveric knees, and (3) to collect sample MR images of undeformed and deformed states of tibiofemoral cartilage free of artifact while using the apparatus within a MRI scanner. An electropneumatic MRI-compatible apparatus was constructed to fit in a clinical MRI scanner, and a slope criterion was defined to indicate the point at which a quasi-steady-state load-displacement response, which would allow the use of CDTR, occurred during cyclic loading of a human knee. The average number of cycles required to reach a quasi-steady-state load-displacement response according to the slope criterion defined herein for three cadaveric knee joints was 356+/-69. This indicates that human knee joint specimens can be cyclically loaded such that deformation is repeatable according to MRI requirements of CDTR. Sample images of tibiofemoral cartilage were obtained for a single knee joint. These images demonstrate the usefulness of the apparatus in a MRI scanner. Thus the results of this study are a crucial step toward developing a MRI-based method to determine the deformations of articular cartilage in whole human cadaveric knees.
Journal of Biomechanical Engineering 09/2009; 131(8):081004. · 1.90 Impact Factor
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ABSTRACT: Non-invasive magnetic resonance imaging (MRI) is a technology that enables the characterization of multiple physical phenomena in living and engineered tissues. The mechanical function of engineered tissues is a primary endpoint for the successful regeneration of many biological tissues, such as articular cartilage, spine and heart. Here we demonstrate the application of MRI to characterize the mechanical function of engineered tissue. Phase contrast-based methods were demonstrated to characterize detailed deformation fields throughout the interior of native and engineered tissue, using an articular cartilage defect model as a study system. MRI techniques revealed that strain fields varied non-uniformly, depending on spatial position. Strains were highest in the tissue constructs compared to surrounding native cartilage. Tissue surface geometry corresponded to strain fields observed within the tissue interior near the surface. Strain fields were further evaluated with respect to the spatial variation in the concentration of glycosaminoglycans ([GAG]), critical proteoglycans in the extracellular matrix of cartilage, as determined by gadolinium-enhanced imaging. [GAG] also varied non-uniformly, depending on spatial position and was lowest in the tissue constructs compared to the surrounding cartilage. The use of multiple MRI techniques to assess tissue mechanical function provides complementary data and suggests that deformation is related to tissue geometry, underlying extracellular matrix constituents and the lack of tissue integration in the model system studied. Specialized and advanced MRI phase contrast-based methods are valuable for the detailed characterization and evaluation of mechanical function of tissue-engineered constructs.
Journal of Tissue Engineering and Regenerative Medicine 07/2009; 3(6):477-85. · 3.28 Impact Factor
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ABSTRACT: Cartilage displacement and strain patterns were documented noninvasively in intact tibiofemoral joints in situ by magnetic resonance imaging (MRI). This study determined the number of compressive loading cycles required to precondition intact joints prior to imaging, the spatial distribution of displacements and strains in cartilage using displacement-encoded MRI, and the depth-dependency of these measures across specimens.
Juvenile porcine tibiofemoral joints were cyclically compressed at one and two times body weight at 0.1 Hz to achieve a quasi-steady state load-displacement response. A 7.0 T MRI scanner was used for displacement-encoded imaging with stimulated echoes and a fast spin echo acquisition (DENSE-FSE) in eight intact joints. Two-dimensional displacements and strains were determined throughout the thickness of the tibial and femoral cartilage and then normalized over the tissue thickness.
Two-dimensional displacements and strains were heterogeneous through the depth of femoral and tibial cartilage under cyclic compression. Strains in the loading direction were compressive and were maximal in the middle zone of femoral and tibial cartilage, and tensile strains were observed in the direction transverse to loading.
This study determined the depth-dependent displacements and strains in intact juvenile porcine tibiofemoral joints using displacement-encoded imaging. Displacement and strain distributions reflect the heterogeneous biochemistry of cartilage and the biomechanical response of the tissue to compression in the loading environment of an intact joint. This unique information about the biomechanics of cartilage has potential for comparisons of healthy and degenerated tissue and in the design of engineered replacement tissues.
Osteoarthritis and Cartilage 06/2009; 17(11):1461-8. · 3.90 Impact Factor
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ABSTRACT: There is a need to align the mechanical axis of the tibia with the axis of loading for studies involving tibiofemoral compression to interpret results and to ensure repeatability of loading within and among specimens. Therefore, the objectives of this study were (1) to develop a magnetic resonance imaging (MRI)-based alignment method for use with apparatuses applying tibiofemoral joint compression, (2) to demonstrate the usefulness of the method by aligning cadaveric knees in an apparatus that could apply tibiofemoral joint compression, and (3) to quantify the error associated with the alignment method. A four degree-of-freedom adjustable device was constructed to allow determination and alignment of the mechanical axis of the tibia of cadaveric knee joints with the axis of loading of an apparatus applying tibiofemoral joint compression. MRI was used to determine the locations of bony landmarks in three dimensions defining the mechanical axis of the tibia relative to an initial orientation of the four degree-of-freedom device. Adjustment values of the device were then computed and applied to the device to align the mechanical axis of the tibia with the axis of a compressive loading apparatus. To demonstrate the usefulness of the method, four cadaveric knees were aligned in the compressive loading apparatus. The vectors describing the mechanical axis of the tibia and the loading axis of the apparatus before and after adjustment of the four degree-of-freedom device were computed for each cadaveric knee. After adjustment of the four degree-of-freedom device, the mechanical axis of the tibia was collinear with the loading axis of the apparatus for each cadaveric knee. The errors in the adjustment values introduced by inaccuracies in the MR images were quantified using the Monte Carlo technique. The precisions in the translational and rotational adjustments were 1.20 mm and 0.90 deg respectively. The MR-based alignment method will allow consistent interpretation of results obtained during tibiofemoral compressive studies conducted using the apparatus described in this paper by providing a well-defined loading axis. The alignment method can also be adapted for use with other apparatuses applying tibiofemoral compression.
Journal of Biomechanical Engineering 01/2008; 129(6):855-62. · 1.90 Impact Factor
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ABSTRACT: Articular cartilage provides critical load-bearing and tribological properties to the normal function of diarthrodial joints. The unique properties of cartilage, as well as heterogeneous deformations during mechanical compression, are due to the nonuniform microstructural organization of tissue components such as collagens and proteoglycans. A new cartilage deformation by tag registration (CDTR) technique has been developed by the authors to determine heterogeneous deformations in articular cartilage explants. The technique uses a combination of specialized MRI methods, a custom cyclic loading apparatus, and image processing software. The objective of this study was to use the CDTR technique to document strain patterns throughout the volume of normal bovine articular cartilage explants during cyclic unconfined compression at two physiologically-relevant applied normal stress levels (1.29 and 2.57 MPa). Despite simple uniaxial cyclic compressive loading with a flat, nonporous indenter, strain patterns were heterogeneous. Strains in the thickness direction (E(yy)) were compressive, varied nonlinearly with depth from the articular surface from a maximum magnitude of 11% at the articular surface, and were comparable despite a 2-fold increase in applied normal stress. Strains perpendicular to the thickness direction (E(xx) and E(zz)) were tensile, decreased linearly with depth from the articular surface from a maximum of 7%, and increased in magnitude 2.5-fold with a 2-fold increase in applied normal stress. Shear strains in the transverse plane (E(xz)) were approximately zero while shear strains in the other two planes were much larger and increased in magnitude with depth from the articular surface, reaching maximum magnitudes of 2% at the articular cartilage-subchondral bone interface. In general, strain patterns indicated that cartilage osteochondral explants exhibited depth-dependent nonisotropic behavior during uniaxial cyclic loading. These results are useful in verifying constitutive formulations of articular cartilage during cyclic unconfined compression and in characterizing the micromechanical environment likely experienced by individual chondrocytes throughout the tissue volume.
Journal of Orthopaedic Research 12/2005; 23(6):1390-8. · 2.81 Impact Factor
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ABSTRACT: Three-dimensional strain fields in articular cartilage subjected to compressive loading can be determined using a recently developed MRI-based cartilage deformation by tag registration technique. The objective of this study was to determine the experimental variables that minimize the technique error, which has not been previously reported. Error (strain bias and precision) was determined using direct experiments and Monte Carlo simulations for four variables: spatial resolution, tag line spacing, applied nominal strain, and number of control points used to describe tag lines in a B-spline model. The important results include the following: (1) bias was not significantly different from zero, (2) precision increased with image resolution and with tag line spacing, (3) precision was independent of applied nominal strain, and (4) error was a minimum (absolute precision = 0.41% strain) for the following values: spatial resolution = 0.05 x 0.05 mm2; tag line spacing = 2.0 mm; control points = 6. With these results the technique can now be used in various applications while minimizing error.
Magnetic Resonance in Medicine 12/2005; 54(5):1290-4. · 2.96 Impact Factor
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ABSTRACT: Roentgen stereophotogrammetric analysis (RSA) can be used to measure changes in anterior-posterior (A-P) knee laxity after anterior cruciate ligament (ACL) reconstruction. Previous measurements of A-P knee laxity using RSA have employed a tibial coordinate system with the origin placed midway between the tips of the tibial-eminences. However, the precision in measuring A-P knee laxity might be improved if the origin was placed on the flexion-extension axis of rotation of the knee. The purpose of this study was to determine whether a center-of-rotation tibial coordinate system with the origin placed midway between the centers of the posterior femoral condyles, which closely approximates the flexion-extension center-of-rotation of the knee, improves the precision in measuring A-P knee laxity compared to the tibial-eminence-based coordinate system. A-P knee laxity was measured using each coordinate system six times in three human cadaveric knees implanted with 0.8-mm diameter tantalum markers. For each laxity measurement, the knee was placed in a custom loading apparatus and biplanar radiographs were obtained while the knee resisted a 44 N posterior shear force and 136 N anterior shear force. A-P knee laxity was determined from the change in position of the tibia, with respect to the femur, resulting from the posterior and anterior shear forces. The precision for each coordinate system was calculated as the pooled standard deviation of A-P knee laxity measurements. The precision of the center-of-rotation coordinate system was 0.33 mm, which was about a factor of 2 better than the 0.62 mm precision of the tibial-eminence coordinate system (p=0.006). The 0.33 mm precision with the center-of-rotation coordinate system suggests that an observed change of either 0.56 mm (i.e. 1.7 standard deviations) or greater in A-P knee laxity over time is a real change and not due to measurement error when the new tibial coordinate system is used and other factors contributing to variability are controlled as was done in this study. Accordingly, clinicians and researchers should consider the use of this alternate tibial coordinate system when making serial measurements of A-P knee laxity using RSA because the improved precision allows for the observation of smaller differences.
Journal of Orthopaedic Research 04/2005; 23(2):327-33. · 2.81 Impact Factor
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ABSTRACT: Articular cartilage is critical to the normal function of diarthrodial joints. Despite the importance of the tissue and the prevalence of cartilage degeneration (e.g., osteoarthritis), the technology required to noninvasively describe nonuniform deformations throughout the volume of the tissue has not been available until recently. The objectives of the work reported in this paper were to 1) describe a noninvasive technique (termed the cartilage deformation by tag registration (CDTR) technique) to determine nonuniform deformations in articular cartilage explants with the use of specialized MRI tagging and image processing methods, 2) evaluate the strain error of the CDTR technique using a custom MRI-compatible phantom material, and 3) demonstrate the applicability of the CDTR technique to articular cartilage by determining 3D strain fields throughout the volume of a bovine articular cartilage explant. A custom MRI pulse sequence was designed to tag and image articular cartilage explants at 7 Tesla in undeformed and deformed states during the application of multiple load cycles. The custom pulse sequence incorporated the "delays alternating with nutations for tailored excitation" (DANTE) pulse sequence to apply tags. This was followed by a "fast spin echo" (FSE) pulse sequence to create images of the tags. The error analysis using the phantom material indicated that deformations can be determined with an error, defined as the strain precision, better than 0.83% strain. When this technique was applied to a single articular cartilage explant loaded in unconfined compression, hetereogeneous deformations throughout the volume of the tissue were evident. This technique potentially can be applied to determine normal cartilage deformations, analyze degenerated cartilage, and evaluate cartilage surgical repair and treatment methodologies. In addition, this technique may be applied to other soft tissues that can be appropriately imaged by MR.
Magnetic Resonance in Medicine 03/2005; 53(2):321-8. · 2.96 Impact Factor
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ABSTRACT: Articular cartilage is critical to the normal function of diarthrodial joints. Despite the importance of the tissue and the prevalence of cartilage degeneration (e.g., osteoarthritis), the technology required to noninvasively describe nonuniform deformations throughout the volume of the tissue has not been available until recently. The objectives of the work reported in this paper were to 1) describe a noninvasive technique (termed the cartilage deformation by tag registration (CDTR) technique) to determine nonuniform deformations in articular cartilage explants with the use of specialized MRI tagging and image processing methods, 2) evaluate the strain error of the CDTR technique using a custom MRI-compatible phantom material, and 3) demonstrate the applicability of the CDTR technique to articular cartilage by determining 3D strain fields throughout the volume of a bovine articular cartilage explant. A custom MRI pulse sequence was designed to tag and image articular cartilage explants at 7 Tesla in undeformed and deformed states during the application of multiple load cycles. The custom pulse sequence incorporated the “delays alternating with nutations for tailored excitation” (DANTE) pulse sequence to apply tags. This was followed by a “fast spin echo” (FSE) pulse sequence to create images of the tags. The error analysis using the phantom material indicated that deformations can be determined with an error, defined as the strain precision, better than 0.83% strain. When this technique was applied to a single articular cartilage explant loaded in unconfined compression, hetereogeneous deformations throughout the volume of the tissue were evident. This technique potentially can be applied to determine normal cartilage deformations, analyze degenerated cartilage, and evaluate cartilage surgical repair and treatment methodologies. In addition, this technique may be applied to other soft tissues that can be appropriately imaged by MR. Magn Reson Med 53:321–328, 2005. © 2005 Wiley-Liss, Inc.
Magnetic Resonance in Medicine 01/2005; 53(2):321 - 328. · 2.96 Impact Factor
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ABSTRACT: Recent magnetic resonance imaging (MRI) techniques have shown potential for measuring non-uniform deformations throughout the volume (i.e. three-dimensional (3D) deformations) in small orthopedic tissues such as articular cartilage. However, to analyze cartilage deformation using MRI techniques, a system is required which can construct images from multiple acquisitions of MRI signals from the cartilage in both the underformed and deformed states. The objectives of the work reported in this article were to 1) design an apparatus that could apply highly repeatable cyclic compressive loads of 400 N and operate in the bore of an MRI scanner, 2) demonstrate that the apparatus and MRI scanner can be successfully integrated to observe 3D deformations in a phantom material, 3) use the apparatus to determine the load cycle necessary to achieve a steady-state deformation response in normal bovine articular cartilage samples using a flat-surfaced and nonporous indentor in unconfined compression. Composed of electronic and pneumatic components, the apparatus regulated pressure to a double-acting pneumatic cylinder so that (1) load-controlled compression cycles were applied to cartilage samples immersed in a saline bath, (2) loading and recovery periods within a cycle varied in time duration, and (3) load magnitude varied so that the stress applied to cartilage samples was within typical physiological ranges. In addition the apparatus allowed gating for MR image acquisition, and operation within the bore of an MRI scanner without creating image artifacts. The apparatus demonstrated high repeatability in load application with a standard deviation of 1.8% of the mean 400 N load applied. When the apparatus was integrated with an MRI scanner programmed with appropriate pulse sequences, images of a phantom material in both the underformed and deformed states were constructed by assembling data acquired through multiple signal acquisitions. Additionally, the number of cycles to reach a steady-state response in normal bovine articular cartilage was 49 for a total cycle duration of 5 seconds, but decreased to 33 and 27 for increasing total cycle durations of 10 and 15 seconds, respectively. Once the steady-state response was achieved, 95% of all displacements were within +/- 7.42 microns of the mean displacement, indicating that the displacement response to the cyclic loads was highly repeatable. With this performance, the MRI-loading apparatus system meets the requirements to create images of articular cartilage from which 3D deformation can be determined.
Journal of Biomechanical Engineering 05/2003; 125(2):180-8. · 1.90 Impact Factor
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ABSTRACT: The capitate is often considered the "keystone" of the carpus, not simply because of its central and prominent position in the wrist, but also because of its mechanical interactions with neighboring bones. The purpose of this study was to determine in vivo three-dimensional capitate kinematics. Twenty uninjured wrists were investigated using a recently developed, non-invasive markerless bone registration (MBR) technique. Surface contours of the capitate, third metacarpal and radius were extracted from computed tomography images of seven wrist positions and the three-dimensional motions of the capitate and third metacarpal were calculated with respect to the radius in wrist flexion-extension and radio-ulnar deviation. We found that in vivo capitate motion does not simply occur about a single pivot point like a universal joint, as demonstrated by non-intersecting rotation axes for different capitate motions. The distance between flexion and ulnar deviation axes was 3.9+/-2.0 mm, and the distance between extension and ulnar deviation axes was 3.9+/-1.4 mm. Furthermore, capitate axes for males tended to be located more distally than axes for females. However, we believe that this result is related to subject size and not to gender. We also found that there is minimal relative motion between the capitate and third metacarpal during these in vivo wrist motions. These findings demonstrate the complexity of capitate kinematics, as well as the different mechanisms through which wrist flexion, extension, radial deviation and ulnar deviation occur.
Journal of Biomechanics 12/2001; 34(11):1429-38. · 2.43 Impact Factor
