A K Haruna

Ahmadu Bello University, Заря, Kaduna, Nigeria

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Publications (15)12.55 Total impact

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    ABSTRACT: In this study, the effect of the methanol extract of Indigofera pulchra Willd. (Papillionaceae) was investigated against castor oil induced diarrheoa. Its effects on perfused isolated rabbit jejunum and guinea pig ileum were also evaluated. The extract produced a dose-dependent protection against the castor oil-induced diarrheoa in mice with the highest protection (100%), obtained at 200 mgkg-1 comparable to that of loperamide (5 mgkg-1), a standard antidiarrhoeal drug. The extract (0.4 - 6.4 mgml-1) produced a concentration relaxation of the rabbit jejunum. However, no observable effect was noticed when the guinea pig ileum was treated. The extract blocked the contractile effect of acetylcholine (2 × 10-8 gml-1) and histamine (4 × 10-7 gml-1) on both rabbit jejunum and guinea pig ileum. Phytochemical screening revealed the presence of flavonoids, tannins, saponins and steroids. The intraperitoneal median lethal dose (LD50) value for the extract was found to be 2154.0 mgkg-1. The results obtained revealed that the extract possesses pharmacologically active compounds with gastrointestinal relaxant and antidiarrhoeal activities and may possibly explain the use of the plant in traditional medicine for the treatment of gastrointestinal disorder.
    Nigerian journal of pharmaceutical sciences. 03/2008; 7(1):86-92.
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    ABSTRACT: Zizyphus spina-christi is used in ethnomedical practice for the treatment of fever. Bio-assay guided investigation of the plant's root bark was initiated and ZS-2A, a fraction from the chloroform extract of the material, eluted with hexane-ethylacetate (50:50) using flash column chromatography, was evaluated for in vivo antiplasmodial activity against Plasmodium berghei in mice. Four-day suppressive, curative effect against established infection and prophylactic models of antiplasmodial studies were used. The fraction (25, 50 and 100 mg/kg, p.o.) showed a potent activity against the parasite in the suppressive and curative tests. The result suggests that ZS-2A may be a promising agent for malaria treatment. © 2007 International Formulae Group. All rights reserved.
    International Journal of Biological and Chemical Sciences 01/2008; 1:281-286.
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    ABSTRACT: Phytochemical screening was carried out on the ethylacetate portion of the ethanolic extract of the leaves of Pseudocedrella kotschyii and then evaluated for its analgesic (acetic acid-induced writhing) and anti-inflammatory (raw egg albumin-induced oedema) activities in mice and rats respectively. Phytochemical screening of the ethylacetate partition portion of ethanolic extract revealed the presence of flavonoids, glycosides and tannins as major chemical constituents. Alkaloids saponins, cardiac glycosides, steroids were not dictated in the extract. The ethylacetate extract (50 and 100 mg/kg i.p) exhibited significant activity (p<0.05) against acetic acid-induced writhing in a dose dependent manner. In the anti-inflammatory activity the ethylacetate extract (50 and 100 mg/kg i.p.) caused a slight effect against the raw egg albumin-induced oedema. The effect was however observed not to be dose dependent. All these effects were compared with standard drug piroxicam (20 mg/kg i.p.).
    African Journal of Traditional, Complementary and Alternative Medicines 01/2007; 5(1):92-6. · 0.52 Impact Factor
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    ABSTRACT: The basis for the use of Zizyphus spina-christi in folkloric practice as a remedy for diarrhoea was investigated in rats. The root bark of the plant material was sequentially extracted and the main antidiarrhoeal activity was traced to a fraction (numbered ZS-4E), eluted with chloroform-methanol (60:40), from the methanol extract. The fraction was tested for antidiarrhoeal activity against castor oil induced-diarrhoea and fluid accumulation, and charcoal meal test in rats. Results show that the fraction (25, 50 and 100 mg/kg, p.o.) exhibited some level of efficacy against the models used which however was not dose-dependent. Phytochemical tests of the methanol extract indicated the presence of tannins, saponins, balsams and carbohydrates. The total tannin content was established to be 18.1 mg/g. © 2007 International Formulae Group. All rights reserved.
    International Journal of Biological and Chemical Sciences 01/2007; 1:15-20.
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    ABSTRACT: The n-butanol soluble part and four chromatographic fractions of the aqueous ethanolic extract of the leaves of Daniellia oliveri were investigated for antimicrobial properties. All fractions showed activity against Staphylococcus aureus. A chromatographic fraction showed significant activity against the fungus Tricophyton rubrum.
    Fitoterapia 01/2005; 75(7-8):729-32. · 2.23 Impact Factor
  • Nigerian Journal of Natural Products and Medicine. 01/2004; 08:66.
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    ABSTRACT: Two phospholipase enzymes NN1 and NN2 were purified from the venom of Naja nigricolis nigricolis Reinhardt to apparent homogeneity. NN1 was purified by a two-step anion-exchange chromatography on DEAE-cellulose column while NN2 was purified by a combination of anion-exchange chromatography and gel filtration on Sephadex G-150. The enzyme NN1 moved homogenously on acrylamide gel as a monomer with a molecular weight of 65 kDa while NN2 was a dimer of 71 kDa. Both enzymes were clearly separated. Both enzymes hydrolyzed L-alpha-phosphatidyl choline with activities of 345.5 for NN1 and 727.8 micromol min(-1) x mg(-1) for NN2. The dimeric 71-kDa enzyme has a higher haemolytic and anticoagulant activity than the monomeric 65-kDa enzyme. It is apparent that the dimeric enzyme has a more pronounced activity than the monomer has, thus toxic activity may be related to the hydrolysis of phospholipids.
    Journal of Biochemical and Molecular Toxicology 02/2003; 17(1):53-8. · 1.60 Impact Factor
  • Nigerian Jourenal of Natural Products and Medicine. 01/2003; 07:13-15.
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    ABSTRACT: The larvicidal activity of light petroleum and ethanolic extracts from leaves of Steganotaenia araliacea (Umbclliferae), a plant traditionally used in pest control in northern Nigeria, was evaluated against the fourth instar larvae of Culex quinqaefasciatus Say. The repellent and antifeedant properties of the plant were evaluated on adult forms of Triholium casteneum Hbst. (red flour beetle). Significant (P < 0.05) repellent and antifeedant effects were noted in wheat powder treated with extract as compared with controls. The results provide sufficient justification for the folkloric usage of the plant in pest control.
    Journal of Herbs. 01/2001; Spices & Medicinal Plants(Vol. 8):51-57.
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    ABSTRACT: The single oral dose pharmacokinetics of paracetamol was studied alone and after coadministration with NIPRISAN In rats. Paracetamol concentrations were measured in rat plasma using UV-spectrophotometer and the data were fitted into an open two-compartment pharmacokinetic model using the computer program (STATIS Version 3.0). Results indicated no significant difference in the absorption of paracetamol between study and control groups but a significant reduction (p < 0.05) at some sampling time as the dose of NIPRISAN was increased double-fold. The pharmacokinetic parameters showed only 1.51 and 7.19% reduction in AUC(0-infinity) and Cmax respectively for paracetamol (20 mg/kg) + NIPRISAN (500 mg/kg). It was thus concluded that the gastric presence of NIPRISAN did not significantly reduce the absorption of paracetamol in rats.
    European Journal of Drug Metabolism and Pharmacokinetics 01/2001; 26(3):201-4. · 1.31 Impact Factor
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    ABSTRACT: Cyperus articulatus is an insect repellant plant commonly found in Northern Nigeria and used traditionally in pest control. The light petroleum and methanol extracts of the plant's rhizome were evaluated against Tribolium casteneum Hbst (the red flour beetle) using standard techniques. The methanol extract showed more antifeedant property than the light petroleum extract, while both the extracts were observed to have similar repellant actions.
    Phytotherapy Research 07/2000; 14(4):281-3. · 2.07 Impact Factor
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    ABSTRACT: The extract of the leaves of Guiera senegalensis was found to detoxify (in vitro) venom from two common northern Nigerian snake species, Echis carinatus and Naja nigricollis, in separate experiments. There was a remarkable reduction in the mortality of albino mice after intra-peritoneal (i.p.) administration of reconstituted venom incubated with the extract, when compared to those challenged with the venom only. The survival of the animals exposed to the venom incubated with the different concentrations of the extract was used as the in vitro detoxification parameter.
    Journal of Ethnopharmacology 04/2000; 69(3):253-7. · 2.76 Impact Factor
  • A K Haruna
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    ABSTRACT: The lyophilized root decoction of Afrormosia laxiflora (Leguminosae), claimed to be beneficial in epilepsy and psychosis, was screened for depressant and anticonvulsant activities using in vivo models. The root decoction, 150-300 mg/kg, showed significant inhibition of motor activity in mice, indicating depressant actions. Similarly, doses of 150-300 mg/kg of this extract significantly diminished the duration of convulsive symptoms, and increased the seizure latency, in both picrotoxin- and electroshock-induced seizures when compared with controls. The results suggest possible beneficial effects of the plant's root and also offer a rational explanation for its folklore usage in epilepsy and related disorders.
    Phytotherapy Research 03/2000; 14(1):57-9. · 2.07 Impact Factor
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    ABSTRACT: The antimicrobial activity of the acetone and methanol extracts of the leaves of Ochna schweinfurthiana F Hoffm (Ochnaceae) obtained through maceration was evaluated using disc diffusion and Nutrient broth dilution techniques. The microorganisms tested were: Staphylococcus aureus ATCC 021001, Bacillus subtilis NCTC 8236, Escherichia coli NCTC 10418, Salmonella typhi ATCC19430, Klebsiella pneumoniae ATCC 10031, Pseudomonas aeruginosa NCTC 6750, Candida albicans ATCC 10231; local hospital isolates: Streptococcus pyogenes, Corynebacterium ulcerans, Neisseria gonorrhoea and methicillin-resistant S. aureus. Susceptibility test results showed that both extracts (600 �g/disc) inhibited growth with a mean zone of inhibition range of 15 - 21 mm against Staphylococcus aureus, S. typhi, K. pneumoniae and P. aeruginosa; no activity was however observed against methicillin resistant S. aureus, N. gonorrhea, C. ulcerans, B. subtilis, E. coli and the only fungus, C. albicans. Sparfloxacin (100 �g/disc) a standard antibiotic inhibited the growth of all the organisms tested with the exception of C. albicans. Preliminary phytochemical screening revealed the presence of flavonoids, saponins and steroids/terpenes. The results suggest that the plant contains bioactive constituent(s) with modest antimicrobial activity and validates the ethno-medical use in wound dressing and other forms of bacterial infections.
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    Bulus Adzu, Abdul Kaita Haruna
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    ABSTRACT: Zizyphus spina-christi (Rhamnaceae) grows wild in tropical Africa and Asia and can be domesticated. It has folkloric usage in pain related ailments throughout these regions. In view of the claimed therapeutic potentials, investigation of the plant’s root bark was initiated in our laboratory. The plant material was first sequentially extracted with hexane, chloroform, ethylacetate and methanol, and in this report, a fraction (numbered) ZS-4D from the methanol extract eluted with 70:30% (chloroform : methanol) using flash column chromatography was apparently traced to be responsible for its main analgesic, and in addition, anti-inflammatory activities. The fraction (25, 50 and 100 mg/kg, i.p.) was tested on chemical (acetic acid-induced writhing, formalin), mechanical (analgesymeter) and thermal (tail-flick) analgesic tests with the aim of elucidating both central and peripherally mediated action in rats and mice. Its anti-inflammatory action against egg albumin-induced hind paw oedema was also tested in rats. Results show that the fraction has some levels of dose related effect on all the models except the tail-flick test in which the activity was not statistically significant.
    African Journal of Biotechnology (ISSN: 1684-5315) Vol 6 Num 11.