Yaping Wang

Chongqing Medical University, Ch’ung-ch’ing-shih, Chongqing Shi, China

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Publications (1)1.72 Total impact

  • Cui Chen · Shubiao Han · Weiwei Liu · Yaping Wang · Guoning Huang
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    ABSTRACT: Objective: The aim of this study was to evaluate the impact of vitrification on mitochondrial membrane potential (ΔΨm) in human metaphase II (MII) oocytes, and the changes of ΔΨm on thawed MII oocytes. Methods: MII oocytes were obtained from clinical IVF cycles when the oocytes were failed to fertilization within 24 h after insemination. All oocytes were randomly divided into 4 groups: non-frozen (fresh group), cultured for 0 h (0 h group), 2 h (2 h group) and 4 h (4 h group) after vitrification/thawing. All oocytes were stained with the ΔΨm-specific probe JC-1 and detected by laser scanning confocal microscope (LSCM) for mitochondrial analysis. Results: The ΔΨm of oocytes was significantly decreased in 0 h and 2 h groups when compared with fresh group (0.93, 1.09 vs 1.34, P < 0.05), but similar between 4 h group and fresh group (1.30 vs 1.34, P > 0.05). Conclusion: In the vitrification/thawing process, the ΔΨm of MII oocytes could have temporally dynamic changes within 2 h after thawing but would be fully recovered after 4 h culture.
    Journal of Assisted Reproduction and Genetics 08/2012; 29(10). DOI:10.1007/s10815-012-9848-1 · 1.72 Impact Factor

Publication Stats

4 Citations
1.72 Total Impact Points


  • 2012
    • Chongqing Medical University
      Ch’ung-ch’ing-shih, Chongqing Shi, China