[Show abstract][Hide abstract] ABSTRACT: The CdSe quantum dot encapsulated by ClpP, a bacterial protease, was synthesized and its electrical transport properties were investigated. ClpP-CdSe was shown to act as a semiconductor and displays p-type behavior in a field effect transistor device. This study reports a platform technology for the development of novel nanoelectrical devices.
[Show abstract][Hide abstract] ABSTRACT: The Hippo pathway controls organ size and tumorigenesis by inhibiting cell proliferation and promoting apoptosis. Kibra was recently identified as a novel regulator of the Hippo pathway. Several of the components of the Hippo pathway are important regulators of mitosis-related cell cycle events. We recently reported that KIBRA is phosphorylated by the mitotic kinases Aurora-A and -B. However, the role KIBRA plays in mitosis has not been established. Here, we show that KIBRA activates the Aurora kinases and is required for full activation of Aurora kinases during mitosis. KIBRA also promotes the phosphorylation of large tumor suppressor 2 (Lats2) on Ser83 by activating Aurora-A, which controls Lats2 centrosome localization. However, Aurora-A is not required for KIBRA to associate with Lats2. We also found that Lats2 inhibits the Aurora-mediated phosphorylation of KIBRA on Ser539, probably via regulating protein phosphatase 1(PP1). Consistent with playing a role in mitosis, siRNA-mediated knockdown of KIBRA causes mitotic abnormalities, including defects of spindle and centrosome formation and chromosome misalignment. We propose that the KIBRA-Aurora-Lats2 protein complexes form a novel axis that regulates precise mitosis.
Journal of Biological Chemistry 08/2012; · 4.57 Impact Factor