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ABSTRACT: Chemotherapy is the main strategy for the treatment of lung cancer. However, sensitivity to chemotherapy, one of the main factors affecting the survival rate of patients with lung cancer, is extremely poor. Forkhead box P3 (Foxp3) is the key regulatory gene for the development and function of CD4+CD25+ regulatory T cells (Tregs). Increased levels of Tregs and Foxp3 expression in the peripheral blood and tumour specimens of cancer patients are associated with tumour progression and poor prognosis. In addition, certain studies have suggested that Tregs may be resistant to conventional chemotherapy and thus, enhance tumour immune evasion. Previous studies have demonstrated that Foxp3 is also expressed within tumour cells and that it may mimic the function of Tregs. Currently, the correlation between the tumour cell expression of Foxp3 and sensitivity to chemotherapy is unclear. Therefore, it was hypothesised that Foxp3 causes resistance to chemotherapeutic agents in lung cancer cells and that it may consequently promote the progression of lung cancer. In the current study, the expression of Foxp3 in mouse Lewis lung cancer (LLC) cells was detected using RT-PCR and immunocytochemistry. The overexpression of Foxp3, which was accomplished by the transient transfection of recombinant pcDNA3.1-Foxp3 or empty plasmids into LLC cells, was confirmed by RT-PCR and western blot analysis. The inhibition of cell proliferation was measured using MTT assay. The expression of multidrug resistance protein 1 (mdr1) mRNA and its protein product, P-glycoprotein (P-gp), were detected by RT-PCR and flow cytometry, respectively. The results revealed that Foxp3 was expressed by LLC cells. The inhibitory rate of cell proliferation in Foxp3-overexpressing LLC cells compared with those transfected with an empty plasmid was significantly decreased following adriamycin (ADM) and mitomycin C (MMC) treatment. The IC50 values of ADM and MMC in Foxp3-overexpressing LLC cells were increased. The expression levels of mdr1 mRNA and P-gp were significantly upregulated in Foxp3 overexpressing LLC cells. These results suggest that Foxp3 reduces the sensitivity of LLC cells to ADM and MMC, thus promoting tumour progression, by upregulating the expression of mdr1 mRNA and P-gp.
Molecular Medicine Reports 11/2012; 6(5):977-82. · 0.42 Impact Factor
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ABSTRACT: Densoviruses (DNVs) infecting arthropods are members of the family Parvoviridae. Here we report the complete genome sequence of a novel DNV with a monosense genome that infects cotton bollworms (Helicoverpa armigera), named HaDNV-1. Alignment and phylogenetic analysis revealed that HaDNV-1 showed high identity with the genus Iteravirus.
Journal of Virology 10/2012; 86(19):10909. · 5.40 Impact Factor
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ABSTRACT: A novel electrochemical aptasensor involving quantum dots-coated silica nanospheres (QDs/Si) and the screen-printed gold electrodes (SPGE) was developed for the detection of thrombin. The screen-printed electrodes with several advantages, including low cost, versatility, miniaturization, and mechanical regeneration after each measurement cycle, were employed. On the other hand, the gold nanoparticles (AuNPs) were electrodeposited on the surface of SPGE to obtain AuNPs/SPGE. And this sandwich format (Apt/thrombin/Apt-QDs/Si) was fixed on the AuNPs/SPGE to fabricate the electrochemical aptasensor. The bound CdTe QDs were dissolved in an acid-dissolution step and were detected by electrochemical stripping analysis. The proposed aptasensor has excellent performance such as high sensitivity, good selectivity and analytical application in real samples. The combination of nanoparticles with the screen-printed electrode is favorable for amplifying electrochemical signals, and useful for large-scale fabrication of the electrochemical aptasensors, which would lay a potential foundation for the development of the electrochemical aptasensor.
Talanta 09/2012; 99:637-42. · 3.79 Impact Factor
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ABSTRACT: Quinones are toxicological substances in inhalable particulate matter (PM). The mechanisms by which quinones cause hazardous effects can be complex. Quinones are highly active redox molecules that can go through a redox cycle with their semiquinone radicals, leading to formation of reactive oxygen species. Electron spin resonance spectra have been reported for semiquinone radicals in PM, indicating the importance of ascorbic acid and iron in quinone redox cycling. However, these findings are insufficient for understanding the toxicity associated with quinone exposure. Herein, we investigated the interactions among anthraquinone (AQ), ascorbic acid, and iron in hydroxyl radical (·OH) generation through the AQ redox cycling process in a physiological buffer. We measured ·OH concentration and analyzed the free radical process. Our results showed that AQ, ascorbic acid, and iron have synergistic effects on ·OH generation in quinone redox cycling; i.e., ascorbyl radical oxidized AQ to semiquinone radical and started the redox cycling, iron accelerated this oxidation and enhanced ·OH generation through Fenton reactions, while ascorbic acid and AQ could help iron to release from quartz surface and enhance its bioavailability. Our findings provide direct evidence for the redox cycling hypothesis about airborne particle surface quinone in lung fluid.
Environmental Science & Technology 08/2012; 46(18):10302-9. · 4.80 Impact Factor
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ABSTRACT: Recurrent graft infection limited the effect of LT, early recognition and prophylaxis of HBV recurrence are very important, and interleukin 28B (IL‐28B) gene was reported to be associated with HBV infection.
To explore the association between IL-28B single-nucleotide polymorphisms (SNPs) and graft re-infection after liver transplantation(LT).
21 recipients with hepatitis B virus(HBV) recurrence and 157 recipients without HBV recurrence were included. We studied three SNPs in the promoter region of IL-28B gene at the positions rs12979860,rs12980275 and rs8099917 by HRM analysis (high-resolution melting curve analysis).
Hepatic allograft dysfunction was more likely to be associated with IL-28B SNPs. However, there was no significant difference in the frequencies of IL-28B gene distribution in recipients with or without HBV recurrence.
IL-28B gene polymorphism may be associated with the prognosis of LT recipients but it needs more experiments.
Gene 08/2012; 508(1):121-4. · 2.34 Impact Factor
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ABSTRACT: To investigate the association between interleukin-10 (IL-10) gene promoter microsatellite polymorphisms and the susceptibility to hepatitis B virus infection in Han, Yi and Yao ethnicities in GuiZhou province.
500 volunteers were selected from Guizhou province. Allelic frequency of IL-10.G and IL-10.R loci was identified by short tandom repeat polymerase chain reaction. The relativity between allelic frequency and HBV infection was analyzed.
Genotype data from H-W analysis on all the IL-10 polymorphisms indicated that it was a random distribution. Very high HBV infection rates were found in the native ethnic minorities of Guizhou province. The overall HBV infection rate among the total population was 67.00%, with the HBV infection rates of Yi nationality in Weining, Yi nationality in Qianxi, Yao nationality in Libo and Han nationality in Libo as 51.85%, 42.86%, 79.52% and 84.30%, respectively. The polymorphisms distribution of IL-10.G and IL-10.R were statistically different among the ethnic groups (P < 0.05). The polymorphisms distribution of IL-10.R had no significant difference between HBV infection group and non-infection group, as well as among HBV natural removal group and non-infected group in all the ethnic groups. The frequency of IL-10.G 459 bp (19CA) was significantly higher in non-infection group than in the infected group (P < 0.05). The frequency of IL-10.G 471 bp (25CA) was significantly higher in the non-infection group than in the HBV natural removal group (P < 0.05). The polymorphisms distribution of IL-10.G did not show significant difference between the HBV infection group and the HBV natural removal group in all the ethnic groups. We did not find any differences in allelic and genotypic frequencies of IL-10.G between infection group and non-infection group in Yi nationality in Weining, and Yao nationality in Libo (P > 0.05), as well as HBV natural removal group and non-infected group (P > 0.05).
The polymorphisms distribution of IL-10.R and IL-10.G did not show significant difference in Yi, Yao and Han ethnics population living in Guizhou province. IL-10.G seemed to influence the susceptibility of HBV infection in Han, Yao and Yi ethnics population of Guizhou province.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 07/2012; 33(7):730-4.
