Bo Song

Zhengzhou University, Cheng, Henan Sheng, China

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Publications (21)38.66 Total impact

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    ABSTRACT: To explore the related ischemic stroke (IS) risk factors in youth and the importance of sleep quality in young ischemic stroke.
    Zhonghua yi xue za zhi. 07/2014; 94(25):1936-1940.
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    ABSTRACT: Recently, PRRT2 gene mutations have been identified as a causative factor of paroxysmal kinesigenic dyskinesia (PKD). However, evidence is still lacking with respect to the genotype to phenotype correlation in PKD patients. We recruited a cohort of PKD patients with or without PRRT2 mutations for the study, and followed them for 6months to observe the response to carbamazepine treatment. Thirty-four participants were included in this study; 16 patients were positive for a hot-spot p.R217Pfs*8 heterozygous PRRT2 gene mutation, while the other 18 patients were negative for PRRT2 gene mutations. PRRT2 mutations were found to be associated with a younger age of onset, bilateral presence and a higher frequency of attacks. Furthermore, the follow-up study revealed that p.R217Pfs*8-positive patients showed dramatic improvement with complete abolition of dyskinetic episodes with carbamazepine treatment, while only 7 of the 18 patients without PRRT2 mutations showed a response to the antiepileptic drug. Our study indicated that positivity for PRRT2 mutation is a predictor of younger age of onset and more frequent of attacks in PKD patients. Interestingly, the presence of PRRT2 mutations also predicted a good response to carbamazepine therapy, especially at low dose. Therefore, genetic testing shows potential clinical significance for guiding the choice of medication for individual PKD cases.
    Journal of the neurological sciences 03/2014; · 2.32 Impact Factor
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    ABSTRACT: Objective. To study risk factors related to ischemic stroke (IS) in youth and the influence of sleep quality on youth ischemic stroke incidence. Methods. 223 patients aged 18 to 45 years who were admitted to Puyang People's Hospital from June 2011 to February 2013 with a first-ever ischemic stroke were selected as the research cases. 158 young people with a normal physical examination were selected as the control group. The Pittsburgh Sleep Quality Index (PSQI) questionnaire was used to analyse the correlation between sleep quality and youth IS incidence. The US National Institutes of Health Stroke Scale (NIHSS) and modified Rankin Scale (MRS) scores were used to assess cases' state of illness and prognosis three months after IS. Results. Univariate and multivariate logistic regression analysis showed that the association of these risk factors with youth IS incidence, from highest to lowest, was hypertension, hyperlipidaemia, smoking history, high homocysteine, the quality of sleep, family history of stroke, and alcoholism. Poor sleep quality ranked fifth among all risk factors and was positively correlated with poor prognosis for youth IS patients. Conclusion. The results of this study showed that sleep quality is an important factor in the pathogenesis and prognosis of youth IS.
    Behavioural neurology 01/2014; 2014:246841. · 1.25 Impact Factor
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    ABSTRACT: The A2DS2 score was recently developed from the Berlin Stroke Registry for predicting in-hospital pneumonia after acute ischemic stroke and performed well in an external validation in the North-west Germany Stroke Registry. It could be a useful tool for risk stratification in clinical practice or stroke trials. We aimed to prospectively validate the predictive value of A2DS2 score in a Chinese stroke population.
    PLoS ONE 01/2014; 9(10):e109665. · 3.53 Impact Factor
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    ABSTRACT: Objective To explore the evaluation of ABCD(3) score in the prediction of stroke risk after transient ischemic attack and the influencing factors of early stroke risks. From October 2010 to September 2012, the data were prospectively collected from patients with transient ischemic attack according to the World Health Organization time-based criteria. ABCD(2) and ABCD(3) scores were available within 7 days of index transient ischemic attack. The predictive outcome was stroke occurrence at 90 days. Risks of stroke stratified according to the score were analysed by Logistic regression. And ABCD(2) and ABCD(3) model were compared by AUROCC. Among 321 eligible patients, 4 cases were lost to follow-up. There were 38(11.8%) patients with ischemic stroke during a 90-day follow-up, including 22(57.9%) males, 16(42.1%) females. There were no occurrence of death, no hemorrhagic stroke. Multivariate analysis revealed that dual TIA (OR = 4.116, 95%CI 1.284-3.195) , history of stroke (OR = 4.022, 95%CI 1.472-10.944) and unilateral weakness (OR = 3.117, 95%CI 1.222-7.948) were the risk factors of early stroke. The ABCD(3) score (0.733, 95% CI,0.0.681-0.780) was statistically higher than that of ABCD(2) score (0.674, 95%CI, 0.619-0.725) (P < 0.05). ABCD(3) may effectively predict early risk of stroke after TIA.
    Zhonghua yi xue za zhi 11/2013; 93(43):3424-7.
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    ABSTRACT: Gordon Holmes syndrome (GHS) is a rare Mendelian neurodegenerative disorder characterized by ataxia and hypogonadism. Recently, it was suggested that disordered ubiquitination underlies GHS though the discovery of exome mutations in the E3 ligase RNF216 and deubiquitinase OTUD4. We performed exome sequencing in a family with two of three siblings afflicted with ataxia and hypogonadism and identified a homozygous mutation in STUB1 (NM_005861) c.737C→T, p.Thr246Met, a gene that encodes the protein CHIP (C-terminus of HSC70 interacting protein). CHIP plays a central role in regulating protein quality control, in part through its ability to function as an E3 ligase. Loss of CHIP function has long been associated with protein misfolding and aggregation in several genetic mouse models of neurodegenerative disorders; however, a role for CHIP in human neurological disease has yet to be identified. Introduction of the Thr246Met mutation into CHIP results in a loss of ubiquitin ligase activity measured directly using recombinant proteins as well as in cell culture models. Loss of CHIP function in mice resulted in behavioral and reproductive impairments that mimic human ataxia and hypogonadism. We conclude that GHS can be caused by a loss of function mutation in CHIP. Our findings further highlight the role of disordered ubiquitination and protein quality control in the pathogenesis of neurodegenerative disease and demonstrate the utility of combining whole exome sequencing with molecular analyses and animal models to define causal disease polymorphisms.
    Human Molecular Genetics 10/2013; · 7.69 Impact Factor
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    ABSTRACT: To perform a meta-analysis on the effect of lowering homocysteine levels via B vitamin supplementation on cerebrovascular disease risk. Using clinical trials published before August 2012 to assess stroke events, we used relative risks (RRs) with 95% confidence intervals (95% CIs) to measure the association between B vitamin supplementation and endpoint events using a fixed-effects model and χ(2) tests. We included 14 randomized controlled trials with 54,913 participants in this analysis. We observed a reduction in overall stroke events resulting from reduction in homocysteine levels following B vitamin supplementation (RR 0.93; 95% CI 0.86-1.00; p = 0.04) but not in subgroups divided according to primary or secondary prevention measures, ischemic vs hemorrhagic stroke, or occurrence of fatal stroke. There were beneficial effects in reducing stroke events in subgroups with ≥3 years follow-up time, and without background of cereal folate fortification or chronic kidney disease (CKD). Some trials that included CKD patients reported decreased glomerular filtration rate with B vitamin supplementation. We conducted detailed subgroup analyses for cyanocobalamin (vitamin B12) but did not find a significant benefit regarding intervention dose of vitamin B12 or baseline blood B12 concentration. Stratified analysis for blood pressure and baseline participant medication use showed benefits with >130 mm Hg systolic blood pressure and lower antiplatelet drug use in reducing stroke risk. B vitamin supplementation for homocysteine reduction significantly reduced stroke events, especially in subjects with certain characteristics who received appropriate intervention measures.
    Neurology 09/2013; · 8.25 Impact Factor
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    ABSTRACT: Transplantation of induced pluripotent stem cells (iPSCs) has shown promising therapeutic effects for ischemic stroke. However, it is not clear if this treatment would promote recovery after intracerebral hemorrhage (ICH). In this study, we investigated the functional outcome of iPSCs transplantation in experimental ICH in rats. IPSCs were derived from an ICH patient's fibroblasts and were injected into the ipsilateral side of ICH in rats. IPSCs transplantation significantly improved the neurological functions after ICH as compared to vehicle and fibroblast injection. The grafted iPSCs migrated into brain tissue around the hematoma, survived after 4 weeks of transplantation, and exhibited the neural cell-specific biomarkers nestin, β-tubulin, and GFAP. Immunohistochemical staining showed that the densities of brain derived neurophic factors (BDNF)-positive cells and vascular endothelial growth factor (VEGF)-positive cells were significantly increased around the hemorrhagic brain tissues of iPSCs-treated rats. In addition, iPSCs treatment increased the protein expression of BDNF and VEGF in the surrounding region of hematoma. These findings demonstrate that the transplantation of ICH patient-derived iPSCs contributes toward the improved neurological function in experimental ICH rats. The mechanisms are possibly due to neuronal replacement and enhanced secretion of neurophic factors. Our data suggest that transplantation of ICH patient-derived iPSCs may be a therapeutic strategy for hemorrhagic stroke.
    Neuroscience Letters 09/2013; · 2.03 Impact Factor
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    ABSTRACT: Specific targeted therapy for intracerebral hemorrhage (ICH), which has high disability and case-fatality rate, is currently not available. Induced pluripotent stem cells (iPSCs) generated from somatic cells of ICH patients have therapeutic potential for individualized cerebral protection. While, whether ICH patient-originated iPSCs could differentiate into neuro-epithelial-like stem (NES) cells and whether such NES cells could improve functional recovery in the hemorrhage-injured brain are unclear. Here, we showed that fibroblasts from an ICH patient can be efficiently reprogrammed to iPSCs by lentiviral vectors carrying defined transcription factors (OCT4, SOX2, KLF4, and c-MYC). These iPSCs have the typical morphology, surface antigens, capability of self-renewal and differentiating into cell types of all three embryonic germ layers that are similar to human embryonic stem cells (hESCs). Using defined serum-free neural differentiation medium, we induced the iPSCs differentiate into NES cells. Subsequently, the NES cells from ICH patient-originated iPSCs were transplanted into the perihematoma of rats with experimental ICH injury. Intriguingly, recovery of neurological dysfunction in experimental ICH rats was observed post-NES cells graftage. Transplanted NES cells migrated to the surrounding area of hematoma, survived and differentiated into neuron-like cells. Our study demonstrates that the transplantation of human iPS-originated NES cells is an effective approach of treating ICH injury and the improvement of neural function is partially due to neuronal replacement and regeneration.
    Neuroscience Letters 05/2013; · 2.03 Impact Factor
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    ABSTRACT: BACKGROUND AND PURPOSE: The Age, Blood Pressure, Clinical Features, Duration, and Diabetes plus Dual TIA (ABCD(3)-I) score is recommended to predict the risk of early stroke after transient ischemic attack. The aim of this study was to validate the predictive value of the ABCD(3)-I score and compare the accuracy of the Age, Blood Pressure, Clinical Features, Duration, and Diabetes (ABCD(2)) and ABCD(3)-I scores in a Chinese population. METHODS: Data were prospectively collected from patients who had transient ischemic attack, as defined by the World Health Organization time-based criteria. ABCD(2) and ABCD(3)-I scores were available within 7 days of the index transient ischemic attack. The predictive outcome was stroke occurrence at 90 days. The receiver-operating characteristic curves were plotted, and the C statistics were calculated as a measure of predictive ability. The comparison of the area under the receiver-operating characteristic curve (area under the curve) was performed by Z test. RESULTS: Among 239 eligible patients, the mean age was 57.4±13.32 years, and 40.2% of the patients were women. The incidence of stroke at 90 days was 12.1%, which ranged from 0% in patients with lower ABCD(3)-I scores (0-3) to 40.91% in those with higher scores of 8 to 13 (P for trend <0.0001). Moreover, the C statistic of ABCD(3)-I scores (0.825; 95% confidence interval, 0.752-0.898) was statistically higher than that of ABCD(2) scores (0.694; 95% confidence interval, 0.601-0.786; P<0.001). CONCLUSIONS: The ABCD(3)-I score had a higher predictive value than the ABCD(2) score for assessing the risk of early stroke after transient ischemic attack in a Chinese population.
    Stroke 03/2013; · 6.16 Impact Factor
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    ABSTRACT: To date there is very few clinical studies published on the cognitive characteristics in patients with SCA3 in China, we sought to evaluate the cognitive function in a cohort of clinically diagnosed and molecularly confirmed patients with SCA3 in China. The neuropsychological tests that were used to evaluate the cognitive function consisted of the Mini-Mental State Examination (MMSE), Clock Drawing Test (CDT), Digit Cancellation Test (DC), Digit Symbol Substitution Test (DSST), Stroop Color-Word Test (SCWT), Trial-Making Test (TMT), Verbal Fluency Test (VFT), and Wechsler Intelligence Scale-Digit Span Test (WISC-DST). The psychiatric symptoms were assessed by the Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD). The severity of motor symptoms was evaluated by the Scale for the Assessment and Rating of Ataxia (SARA). 15 patients with genetically confirmed SCA3 and 15 normal control subjects were enrolled in the study. There was no significant difference in age, gender or educational level among these 2 groups. CDT, DC, DSST, SCWT, TMT, VFT were significantly more impaired in patients with SCA3 than those in the control group. There was no significant difference in the MMSE, DST, HAMA and HAMD between SCA3 patients and controls. In conclusion, our study demonstrates that patients with SCA3 in China present cogintive impairments, manifesting mainly as executive and visuospatial dysfunction.
    Life Science Journal 03/2013; 10(1):1655-1659. · 0.17 Impact Factor
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    ABSTRACT: Recent evidence has implicated the gene for phosphodiesterase 4D (PDE4D) as susceptibility gene for ischemic stroke (IS) in Icelandic population. However, there are few reports on the associations between PDE4D gene polymorphisms and IS in Chinese individuals. The present study aimed to investigate the possible association of genetic polymorphisms in PDE4D gene with IS in Henan Han population. A total of 400 patients with IS and 400 matched controls were examined using a case-control design. Two single nucleotide polymorphism (SNPs) (rs918592 and rs2910829) in PDE4D gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odds ratios (OR) and 95% confidence intervals (95%CI) were calculated to test the association between the genetic factors and IS. Genetic parameter and association studies were carried out with SPSS 16.0. Among the two SNPs tested, the rs918592 was significantly associated with IS (OR: 1.351, 95%CI: 1.110 - 1.645), especially in male patients (OR: 1.427, 95%CI: 1.105 - 1.844). Haplotype analysis showed that A-T was associated with an increased risk of the IS (OR: 2.114, 95%CI: 2.005 - 2.230) while G-T was associated with decreased risk of IS (OR: 0.419, 95%CI: 0.302 - 0.583). Protecting effect of haplotype G-T was also significant in males (OR: 0.264, 95%CI: 0.162 - 0.431). The present study demonstrated a strong association of rs918592 with IS. Haplotype A-T increased the risk of IS while haplotype G-T had a protective effect in Henan Han population. The association was sex-dependent with male patients showing stronger effect.
    Chinese medical journal 07/2012; 125(13):2255-9. · 0.90 Impact Factor
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    ABSTRACT: Alzheimer's disease (AD) is a progressive neurodegenerative disease, characterized by amyloid-beta (Aβ) deposition and neurofibrillary tangles. Numerous microRNAs have been found to play crucial roles in regulating Aβ production in the process of AD. Previous investigations have reported lower levels of many microRNAs in AD patients and animal models. Here, we examined the role of miR-195 in the process of Aβ formation. Bioinformatics' algorithms predicted miR-195 binding sites within the beta-site APP cleaving enzyme 1 (BACE1) 3'-untranslated region (3'-UTR), and we found the level of miR-195 to be negatively related to the protein level of BACE1 in SAMP8 mice. We confirmed the target site in HEK293 cells by luciferase assay. Overexpression of miR-195 in N2a/WT cells decreased the BACE1 protein level, and inhibition of miR-195 resulted in increase of BACE1 protein level. Furthermore, overexpression of miR-195 in N2a/APP decreased the level of Aβ, while inhibition of miR-195 resulted in an increase of Aβ. Thus, we demonstrated that miR-195 could downregulate the level of Aβ by inhibiting the translation of BACE1. We conclude that miR-195 might provide a therapeutic strategy for AD.
    Brain research bulletin 06/2012; 88(6):596-601. · 2.18 Impact Factor
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    ABSTRACT: To examine the correlation between hypertension with hyperhomocysteinemia and prognosis of ischemic stroke. A total of 634 patients with acute ischemic stroke confirmed by computed tomography or magnetic resonance imaging were recruited at Department of Neurology, First Affiliated Hospital, Zhengzhou University from January 1, 2007 to May 30, 2010. Their NIHSS (National Institute of Health Stroke Scale) scores were evaluated on admission. And their baseline profiles and probable prognostic factors were recorded. Recovery was assessed by modified Rankin score (MRS) during a 6-month follow-up. Multivariate Logistic regression was performed for statistical analysis. Among them, 32 became lost to follow-up. There were 197 females (32.7%) and 405 males (67.3%) with an average age of (59 ± 13) years (range: 19 - 92). The average level of homocysteine was (19 ± 11) µmol/L. There were 343 (57%) patients with hypertension and MRS ≥ 3 was in 145 (31%) patients. Logistic regression analysis showed that significant correlations existed between H-type hypertension and 6-month MRS (P = 0.012, OR = 2.566, 95%CI: 1.299 - 5.357) when homocysteine ≥ 15 µmol/L was defined for hyperhomocysteinemia. And there was a total sum of interaction between hypertension and hyperhomocysteinemia. The parameters of relative excess risk of interaction (RERI), attributable proportion due to interaction (AP) and synergy index (S) were 0.683, 26.61% and 1.59 respectively. Other parameters with significant prognostic correlations included age, history of stroke, NIHSS score on admission and diastolic blood pressure on admission. At the homocysteine level of ≥ 15 µmol/L, H-type hypertension and 6-month MRS have significant correlations. And H-type hypertension is a risk factor for the prognosis of ischemic stroke. When hypertension and hyperhomocysteinemia coexist, there is a total sum of interaction.
    Zhonghua yi xue za zhi 05/2012; 92(17):1183-6.
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    ABSTRACT: Aims: To establish HSV2 VP16 targeting shRNA-expressing cell lines and investigate the antiviral effect of shRNA targeting HSV2 VP16. Methods: The cell lines Vero-shRNAs and negative-control Vero-shCON were established. Their inhibition effects on VP16 mRNA expression were tested by real-time fluorescent quantitative polymerase chain reaction (PCR) and their antiviral effects were evaluated by yield reduction assay. The influence of passage numbers on the inhibition ability of cell lines was researched. Results: Vero-shRNA24 targeting the upper stream, Vero-shRNA642 targeting the lower stream and Vero-shCON were established. Vero-shRNA24, Vero-shRNA642 and Vero-shRNA24 + 642 could reduce the VP16 mRNA significantly. Vero-shRNA24 was the most efficient. The HSV2 titers in Vero and Vero-shCON were the highest at 72 h after infection, and started decreasing thereafter. The viral titers of the Vero-shRNA groups reached a peak after 84 h and the highest titers were lower than in the Vero group. The inhibiting effect on VP16 mRNA expression and viral replication of Vero-shRNA24 cell lines of passages 10 and 20 were not significantly different from the primary cell line. Although of no statistical significance, the passage 50 cell line showed decreased inhibiting ability. Conclusions: Recombinant cell lines expressing shRNA targeting HSV2 VP16 were established. They can stably inhibit HSV2 VP16 mRNA expression and viral replication within passage 50.
    Intervirology 01/2012; 55(6):426-34. · 1.89 Impact Factor
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    ABSTRACT: The study aimed to construct the amplicon vector of HSV-1 strain HF and explore its universal package function between different serotypes of HSV. OriS and pac elements were obtained by enzyme digestion from the Plasmid BAC-HSV-1 strain HF and sequenced. With red fluorescence (DsRed) as a reporter gene, the amplicon vector of HSV-1 strain HF was constructed based on pSilencer2.0-U6. The amplicon vector was transfected into Vero cells by lipofectamine 2000, then packaged by HSV-1 strain HF and HSV-2 strain HG52 as helper virus separately. The supernatant was collected after cytopathic effect. Red fluorescence was observed in Vero cells reinfected by the supernatant. In this study,the amplicon vector of HSV-1 strain HF was successfully constructed and it could be packaged by HSV-1 strain HF and HSV-2 strainHG52.
    Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] 09/2011; 27(5):409-15.
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    ABSTRACT: In this study, we incorporated the lambda phage based specific recombination sites attR into HSV-1 replication-defective vector by using Red recombineering, and constructed the HSV-1 replication-defective vector BAC-HSV1-HF-△ICP27-attR-GK. In addition, a red fluorescence reporter gene DsRed was introduced to the vector to construct the BAC-HSV1-HF-△ICP27-attB-DsRed by LR recombination approach. To conduct research on the expression of exogenous gene integrated to the vector, the plasmid BAC-HSV1-HF-△ICP27-attB-DsRed was transfected to the 2-2 Vero cells, 72hr later, CPE and the red fluorescence protein were observed. These results indicated that we successfully constructed HSV-1 replication-defective vector BAC-HSV1-HF-△ICP27-attR-GK which carrying LR recombination specific sites attR, and the vector can incorporate exogenous gene by a one-step LR recombination in vitro. This method simplified the procedure of site-directed integration of exogenous gene into HSV-1 replication-defective vectors, and greatly facilitated the research of HSV-1 derived vectors for gene therapy. [Qingzhi Wang, Bo Song, Xinjing Liu, Zhiqiang Han, Jiameng Lu, Ting Yang, Chenyang Jiang, Xiaolu Zhang, Chandra Avinash, Shilei Sun, Yuming Xu. Construction of a HSV-1 strain HF Based Replication Defective Vector with LR-Recombination
    Life Science Journal,. 08/2011;
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    ABSTRACT: To construct the plasmid of BAC-HSV-1 with GFP reporter gene and research the biological property of its infectious progeny virus. We constructed the plasmid C223-UL43-left-arms-UL47-right-arms which carried the homologous sequences of HSV-1. Liposome embedding method was used to transfect HSV-1 genome and the plasmid C223-UL43-left-arms-UL47-right-arms linearized by Mlu I digestion into Vero cells. After the successful homologous recombination in the eukaryotic cells, the recombinant BAC-HSV-1 with GFP reporter gene was generated. Then, the positive CPE were taken by plaque purification and by hirt extraction during the moment of the circularization of HSV-1 DNA, and the plasmid of BAC -HSV-1 was acquired. Electroporation was used to transfect the BAC -HSV-1 into DH10B, and then the single colonies of interest were confirmed both by MluI digestion and PCR. Experimental group and the control group cells were given BAC-HSV-1 plasmid and HSV-1 genomic DNA respectively to produce the BAC-HSV-1 and HSV-1 progeny virions. Vero cells were inoculated with the progeny virions at MOI = 0.1 and then a TCID50 assay was performed to determine the titers of virons in the two groups at 48 hours post inoculation. The plasmid BAC-HSV-1 was successfully constructed by the restriction enzyme analysis and the PCR. The titers of progeny virions were calculated by the TCID50 assay. No significant difference in the titers of virions between two groups was observed (P > 0.05). The infectious BAC-HSV-1 shuttle virus/plasmid between eukaryotic and prokaryotic cells was successfully constructed.
    Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] 05/2011; 27(3):238-43.
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    ABSTRACT: To explore the effect of lentivirus-mediated APP695 RNAi on Aβ level in the cortical neurons of APP695 transgenic mice. The pFU-GW-iRNA plasmids expressing the RNAi sequences of human APP695 gene were confirmed by PCR (polymerase chain reaction) and sequencing. The recombined plasmids in combination with pHelper 1.0 and Helper 2.0 plasmids were transfected into 293T cells so as to construct the lentiviral vectors of RNA interference of APP695 gene. Virus in supernatant was collected and the viral titer measured. The cortical neurons of APP695 transgenic mice were infected with the packaged lentivirus. And it was designated as APP695-RNAi group. Cells infected with APP695-NC was designated as NC group and non-infected cells as CON group. The levels of APP695 mRNA were detected by real-time quantitative PCR. The APP695 protein was detected by Western blot and the levels of Aβ40 and Aβ402 were measured by ELlSA (enzyme-linked immunosorbent assay). PCR and DNA sequencing demonstrated that the inserted sequences of APP695 shRNA were correct. The viral titer was 5 × 10(8) TU/ml. After the infection of APP695 shRNA recombinant lentivirus, the expression level of APP695 mRNA in the cortical neurons of APP695 transgenic mice decreased significantly (inhibition rate: 76.70%) versus NC and CON groups (P < 0.001). The lowered expression of APP695 proteins was consistent with APP695 mRNA. The levels of Aβ40 were (184 ± 15) ng/L, (647 ± 30) ng/L and (656 ± 40) ng/L in APP695-RNAi, NC and CON groups respectively. As compared with NC and CON groups, the levels of Aβ40 in APP695-RNAi group decreased significantly (P < 0.001). The levels of Aβ42 were (19.2 ± 1. 9) ng/L, (67.6 ± 6.0) ng/L and (68.6 ± 7.0) ng/L in APP695-RNAi, NC and CON groups respectively. As compared with NC and CON groups, the levels of Aβ42 decreased significantly in APP695-RNAi group(P < 0.001). The inhibition of APP695 by lentivirus-mediated RNAi effectively decreases the levels of Aβ40 and Aβ42 in the cortical neurons of APP695 transgenic mice.
    Zhonghua yi xue za zhi 12/2010; 90(45):3225-30.
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    ABSTRACT: HSV-1, a neurotropic virus, always leads to severe nervous symptoms. It is hard to completely eradicate the latent viruses after conventional therapy so that recurrence is inevitable. ICP is a key regulator for HSV replication and transcription that determines the cytolytic infection or latent state. In search of new anti-virus strategy targeting HSV-1ICP4, two pairs of siRNA were designed, and a recombinant eukaryotic lentiviral expression plasmid pLKO-puro(r)-hU6-siRNA was constructed. Vero cells were transfected with the designed siRNAs by Lipofectamine 2000 and four stable monoclonal cell lines were established by puromycin screening method. The ICP4 expression at mRNA level was detected with real-time PCR, and the HSV-1 replication was measured with TCID50 assay. SiRNA was shown as an effective way to inhibit the expression of ICP4 in monoclonal cell lines. Meanwhile, HSV-1 replication was significantly inhibited when ICP4 was shut down by siRNA. We conclude that siRNA targeting ICP4 attenuates HSV-1 replication. Further more, multi-site siRNAs show stronger inhibitory effect on viral replication, which may be an effective and feasible approach for biological anti-viral drugs.
    Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] 05/2010; 26(3):163-9.