Yonghong Wang

Chongqing Center for Disease Control and Prevention, Ch’ung-ch’ing-shih, Chongqing Shi, China

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Publications (5)19.3 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: As one of the most sensitive detection mode for CE, chemiluminescence (CL) detection is less developed compared to some other detection modes such as MS and LIF, despite its low equipment cost and simple design. The fact is partly due to the limitation of CL systems suitable for CE. In this paper, a highly sensitive CE-CL strategy was established for the determination of dopamine (DA) and cimbuterol (CM) using acridinium ester as derivatization reagent. Catalyst was not required in this CL detection system. Also, a good sensitivity was obtained due to its quite low background and strong signal. Under the optimal conditions, the presented method has been successfully applied to analyze DA and CM with LODs (S/N = 3) of 2.0 and 0.50 ng/mL, respectively. The linear ranges were 5.0–1500 ng/mL and 2.0–1000 ng/mL for DA and CM, respectively. To validate our method, the levels of DA in human urine samples were detected by this method, and the results showed acceptable accordance with those of ELISA. All the analysis procedure could be completed in 400 s, and the results showed satisfactory sensitivity and selectivity. The approach could also be further extended to rapid analysis of many other compounds including primary and second amines in clinical medicine and biopharmaceutical analysis using acridinium ester as CL derivatization reagent.
    Electrophoresis 04/2014; 35(7). · 3.26 Impact Factor
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    ABSTRACT: As a familiar β2-agonist, clenbuterol (CLB) was frequently reported to be illegally abused as a “lean meat agent” for livestock breeding, since it could improve growth rate and inhibit fat synthesis. Due to its potential hazard to human health, it has been forbidden as growth promoter for food-producing animal feeding in most countries. Therefore, the routine monitoring of CLB abuse is exceedingly necessary for food safety purpose. We herein report an ultrasensitive electrochemiluminescent immunosensor, which is firstly applied for assay of trace amount of CLB residue in swine urine. In this investigation, gold nanoparticles doped chitosan composite film was utilized for antigen immobilization, and Ru(bpy)32+-labeled antibody was utilized as the tracer antibody. With a competitive immunoassay format, good analytical performance was obtained. The proposed method shows a linear range of 0.010–1.0 ng/mL, with a limit of detection of 0.0050 ng/mL for CLB, which is evidently lower than that of most previous reports. This method is suitable for screening of trace amount of CLB residue, due to its simple manipulation, short assay time, and high detection sensitivity, and exhibits great promise in food safety and agonist monitoring.
    Sensors and Actuators B Chemical 11/2012; 174:355–358. · 3.84 Impact Factor
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    ABSTRACT: Nowadays, β(2)-agonists are abused illegally as "lean meat agents" for food-producing animals, and cause increasing food-safety accidents in some countries. Due to their hazard to the human health, "lean meat agents" are banned in most countries and required to be routinely monitored. We herein report a disposable electrochemiluminescent immunosensors array for near-simultaneous assay of multiple β(2)-agonist residues in swine urine, by using ractopamine and salbutamol as the models. In this investigation, a screen-printed carbon electrodes array was assembled and acted as the substrate of the immunosensors array. Then the immunosensors array was constructed by site-selectively immobilizing the antigens of ractopamine and salbutamol on the working electrodes of array. After the competitive immuno-binding, with the aid of a homemade single-pore-four-throw switch, the electrochemiluminescent signals of the two β(2)-agonists were sequentially detected using a non-array detector. The limits of detection for ractopamine and salbutamol were 8.5 and 17pg/mL, respectively, which were much lower than those of the most previous reports. Compared with other routine methods based on chromatography and ELISA, this method is more suitable for screening of multiple β(2)-agonists in quantities of samples, owing to its merits of low cost, user-friendliness and high throughput, and shows great promise in food safety and agonist surveillance.
    Biosensors & Bioelectronics 07/2012; 39(1):311-4. · 6.45 Impact Factor
  • Zhifeng Fu, Lin Wang, Yonghong Wang
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    ABSTRACT: Numerous drugs are carboxylic acid derivatives containing amino group, and hydrolysis reaction of these agents often generates toxic amines. Thus, the detection of amine impurity is of great importance in drug quality control of these amino group-containing ester and amide. A capillary electrophoresis method coupled with end-column electrochemiluminescent detection based on tris(2,2'-bipyridyl)ruthenium(II) system was proposed for the analysis of N,N-dimethyl ethanolamine (DMEA, the degradation product of meclophenoxate) in the presence of its precursor. Baseline separation of DMEA and meclophenoxate can be easily achieved under the selected conditions. DMEA can be assayed within 3 min over the concentration range of 5.0x10(-8) to 3.0x10(-6) mol L(-1) with a detection limit of 2.0x10(-8) mol L(-1) at the signal-to-noise ratio of 3. The relative standard deviations of the signal intensity and the migration time were less than 5.3 and 2.5% for a standard sample containing 1.0x10(-7) mol L(-1) DMEA (n=5), respectively. The presented method has been successfully applied for the profiling of DMEA resulting from the hydrolysis of meclophenoxate in commercial formulations. A primary stability investigation of meclophenoxate in aqueous solution was also carried out at different temperatures, and the results showed that the degradation of meclophenoxate accelerated at the higher temperature.
    Analytica chimica acta 05/2009; 638(2):220-4. · 4.31 Impact Factor
  • Zhifeng Fu, Ying Liu, Lin Wang, Yonghong Wang
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    ABSTRACT: A simple, rapid and sensitive method has been proposed for simultaneous electrogenerated-chemiluminescent detection of quinolone residues in biological fluid after effective separation by capillary electrophoresis. Enrofloxacin, levofloxacin and ciprofloxacin can be assayed in the range of 3.0×10−8–5.0×10−6gmL−1 within 10min. The relative standard deviations of the signal intensity and the migration time were less than 4.9 and 2.4% for a standard sample containing 1.0×10−7gmL−1 of each quinolone (n=5), respectively. The presented method has been successfully applied to determine the amounts of quinolones in pig urine after clean-up by C18 solid phase extraction column.
    Chromatographia 69(9):1101-1105. · 1.44 Impact Factor

Publication Stats

10 Citations
19.30 Total Impact Points

Institutions

  • 2009–2014
    • Chongqing Center for Disease Control and Prevention
      Ch’ung-ch’ing-shih, Chongqing Shi, China
  • 2009–2012
    • Southwest University in Chongqing
      Pehpei, Chongqing Shi, China