Y Zhao

Fudan University, Shanghai, Shanghai Shi, China

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Publications (9)27.8 Total impact

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    ABSTRACT: Tripartite motif-containing protein 35 (TRIM35) is a member of RBCC family, which has a highly conserved order consisting of a RING domain followed by one or two B-Box domains and then a coiled-coil domain. We previously identified TRIM35 as a novel tumor suppressor in human hepatocellular carcinoma (HCC). However, the molecular mechanism that TRIM35 uses to suppress tumorigenicity is largely unknown. Pyruvate kinase isoform M2 (PKM2) has been demonstrated to have a central role in metabolic reprogramming during cancer progression. Phosphorylation of PKM2 tyrosine residue 105 (Y105) regulates PKM2 to provide a metabolic advantage to tumor cells, thereby promoting tumor growth. In the present work, mass spectrometry analysis demonstrated an interaction between TRIM35 and PKM2. Co-IP experiments confirmed that TRIM35 interacts with PKM2 and that the coiled-coil domain is required for such an interaction. Furthermore, the coiled-coil domain mediates decreases in the Warburg effect and in the cell proliferation of HCC cells. In addition, TRIM35 suppresses the tumorigenicity of HCC cells through the blockade of PKM2 Y105 phosphorylation. Collectively, our data reveal a new function for TRIM35, which is to regulate the Warburg effect and tumorigenicity through interaction with PKM2 in HCC.Oncogene advance online publication, 29 September 2014; doi:10.1038/onc.2014.325.
    Oncogene 09/2014; · 8.56 Impact Factor
  • J. Shi, X. Yue, Y. Zhao
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    ABSTRACT: This article studies operations sequencing for a multi-stage production inventory system with lead times under predictable (deterministic) yield losses and random demand. We consider various cases with either full or partial release of work-in-process inventories, for either pre-operation or post-operation cost structures, and under either the total discounted or average cost criteria. We derive necessary and sufficient criteria for the optimal sequence of operations in all cases. While the criteria differ in their specific forms, they all lead to the same principal: those operations with (1) lower yields, (2) lower processing costs, (3) longer lead times, and (4) lower inventory holding costs should be placed higher upstream in the system.
    Naval Research Logistics Quarterly 03/2014; 61(2):144-154.
  • H. Wei, X. Yue, Y. Zhao, L. Li, D. Jia
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    ABSTRACT: With the nature ultra-low permeable cores from Changqing Oilfield, the simulated experiments of nitrogen drive after water flooding have been accomplished for the ultra–low-permeability reservoir. The injection pattern of nitrogen includes direct gas injection, water/gas alternating injection and pulse gas injection. The results show that direct injection could not develop the ability that nitrogen enters into the local tight area to improve the microsweep efficiency, because the good mobility generates gas channeling in some local high-permeable zone. Subsequent direct nitrogen injection after water drive only enhances the oil recovery ratio by 3.65%. The dispersion degree of oil, water, and gas is much higher for water/gas alternating injection after water injection, and the capillary force is the main resistance to suppress the rapid gas flow validly in local high-permeable zone. Nitrogen is enforced to drive the residual oil in local tight area or micro pore system. Water/gas alternating injection after water flooding enhances oil recovery ratio by 16.37%. Pulsing injection can take advantage of the elastic energy of the high-pressure nitrogen and pressure disturbance effect, which makes pressure redistribution and fluid deallocation between local high-permeability zone and local compact area. With pulse injection, oil in local tight region also can be driven by nitrogen, and the recovery ratio is enhanced by 15.94%.
    Petroleum Science and Technology 01/2013; 31(21). · 0.30 Impact Factor
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    ABSTRACT: The purpose of this study was to define the roles of miR-181a in determining sensitivity of cervical cancer to radiation therapy, to explore the underlying mechanism and to evaluate the potential of miR-181a as a biomarker for predicting radio-sensitivity. Tumor specimens from 18 patients with a histological diagnosis of squamous cervical carcinoma (stage IIIB) were used in the micro-RNA profiling and comparison. These patients never received any chemotherapy before radiation therapy. Human cervical cancer cell lines, SiHa and Me180, were used in vitro (cell culture) and in vivo (animal) studies. Transfection of tumor cells with the mimic or inhibitor of miR-181a, and reporter gene assay, were performed to investigate the role of miR-181a in determining radio-sensitivity and the target gene. Higher expression of miR-181a was observed in human cervical cancer specimens and cell lines that were insensitive to radiation therapy, as compared with sensitive cancer specimens and the cell lines. We also found that miR-181a negatively regulated the expression of PRKCD, a pro-apoptotic protein kinase, via targeting its 3'-untranslated region (UTR), thereby inhibiting irradiation-induced apoptosis and decreasing G(2)/M block. The role of miR-181a in conferring cellular resistance to radiation treatment was validated both in cell culture models and in mouse tumor xenograft models. The effect of miR-181a on radio-resistance was mediated through targeting the 3'-UTR of PRKCD gene. Thus, the expression level of miR-181a in cervical cancer may serve as a biomarker for sensitivity to radiation therapy, and targeting miR-181a may represent a new approach to sensitizing cervical cancer to radiation treatment.Oncogene advance online publication, 30 July 2012; doi:10.1038/onc.2012.323.
    Oncogene 07/2012; · 8.56 Impact Factor
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    ABSTRACT: Cyclin-dependent kinase inhibitor 1A (CDKN1A), also known as p21Cip1/Waf1, is a master downstream effector of tumor suppressors. In this study, we experimentally demonstrate through a high-throughput luciferase reporter screen that p21Cip1/Waf1 can be directly targeted by nearly 28 microRNAs (miRNAs). The results were further confirmed by a series of mutational analyses and luciferase reporter assays. These 28 miRNAs can substantially inhibit p21Cip1/Waf1 expression, predominantly at translational level. Many of these miRNAs were upregulated in cancers and might serve as modulators of oncogenesis. Furthermore, 8 of these 28 p21-regulating miRNAs are located in the chromosome 19 miRNA cluster, the largest miRNA gene cluster in humans, and they can clearly promote cell proliferation and cell-cycle progression in choriocarcinoma cells. In conclusion, our screening strategy provides an alternative approach to uncovering miRNA modulators of an individual mRNA, and it has identified multiple miRNAs that can suppress p21Cip1/Waf1 expression by directly targeting its 3' untranslated region.
    Oncogene 03/2010; 29(15):2302-8. · 8.56 Impact Factor
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    ABSTRACT: In order to develop a mathematical model of the retrospective dosimetry method for the cohort study of Chinese medical diagnostic X ray workers, more than 608 X ray machines, and 1632 various working places were measured by the simulation. A data bank of the measurement results was established, and a statistical analysis was conducted. Some main results of the analysis are reported.
    Radiation Protection Dosimetry 05/1998; · 0.91 Impact Factor
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    ABSTRACT: In order to provide reasonable and reliable dose information for the cohort study of Chinese medical diagnostic X ray workers, a retrospective dosimetry method was established. Based on the principal character of occupational exposure of the workers, a mathematical model was developed and relative coefficients of the model were determined, and the model was computerised. For dose estimation by this model, a sampling survey of occupational history of the workers was conducted, and a data bank on occupational history was established. Using the data bank and the model, dose analysis was conducted. Some of the main results are reported here.
    Radiation Protection Dosimetry 05/1998; · 0.91 Impact Factor
  • T YANG, P ZHU, G DAI, S BEI, Y ZHAO
    Journal of Molecular and Cellular Cardiology - J MOL CELL CARDIOL. 01/1987; 19.
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Publication Stats

126 Citations
27.80 Total Impact Points

Institutions

  • 2014
    • Fudan University
      • Department of Oncology
      Shanghai, Shanghai Shi, China
  • 2013
    • China University of Petroleum
      Ch’ang-p’ing-ch’ü, Beijing, China
  • 2010–2012
    • Shanghai Cancer Institute
      Shanghai, Shanghai Shi, China