M G Chen

Publications (3)2.76 Total impact

• Article: Gene sequencing for identification of Paragonimus eggs from a human case.

  Z S Chang, B Wu, D Blair, Y N Zhang, L Hu, S H Chen, M G Chen, Z Feng, G M Davis
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  ABSTRACT: To identify the etiologic agent from a paragonimiasis patient using molecular techniques. The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product. ITS2 genes from eggs of Paragonimus westermani and Pagumogonimus skrjabini (both from animal hosts) were also sequenced for comparison. In addition, morphological comparisons were made with the eggs of the two species. The ITS2 gene from the human case was 100% identical with the sequence from the eggs of P. westermani from an experimentally infected dog but only 92% identical with the sequence from the eggs of P. skrjabini. Morphologically, the eggs from the human case more resembled those from P. westermani infected dog. The patient was diagnosed to be suffered from paragonimiasis westermani by gene sequence analysis.
  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 02/2000; 18(4):213-5.
• Source

  Available from: Guevara Angel

  Article: A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen.

  D Blair, B Wu, Z S Chang, X Gong, T Agatsuma, Y N Zhang, S H Chen, J X Lin, M G Chen, J Waikagul, A G Guevara, Z Feng, G M Davis
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  ABSTRACT: The status of the genera Euparagonimus Chen, 1963 and Pagumogonimus Chen, 1963 relative to Paragonimus Braun, 1899 was investigated using DNA sequences from the mitochondrial cytochrome c oxidase subunit I (CO1) gene (partial) and the nuclear ribosomal DNA second internal transcribed spacer (ITS2). In the phylogenetic trees constructed, the genus Pagumogonimus is clearly not monophyletic and therefore not a natural taxon. Indeed, the type species of Pagumogonimus, P. skrjabini from China, is very closely related to Paragonimus miyazakii from Japan. The status of Euparagonimus is less obvious. Euparagonimus cenocopiosus lies distant from other lungflukes included in the analysis. It can be placed as sister to Paragonimus in some analyses and falls within the genus in others. A recently published morphological study placed E. cenocopiosus within the genus Paragonimus and probably this is where it should remain.
  Journal of Helminthology 01/2000; 73(4):295-9. · 1.38 Impact Factor
• Article: A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen

  D. Blair, B. Wu, Z.S. Chang, X. Gong, T. Agatsuma, Y.N. Zhang, S.H. Chen, J.X. Lin, M.G. Chen, J. Waikagul, A.G. Guevara, Z. Feng, G.M. Davis
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  ABSTRACT: The status of the genera Euparagonimus Chen, 1963 and Pagumogonimus Chen, 1963 relative to Paragonimus Braun, 1899 was investigated using DNA sequences from the mitochondrial cytochrome c oxidase subunit I (CO1) gene (partial) and the nuclear ribosomal DNA second internal transcribed spacer (ITS2). In the phylogenetic trees constructed, the genus Pagumogonimus is clearly not monophyletic and therefore not a natural taxon. Indeed, the type species of Pagumogonimus,P. skrjabini from China, is very closely related to Paragonimus miyazakii from Japan. The status of Euparagonimus is less obvious. Euparagonimus cenocopiosus lies distant from other lungflukes included in the analysis. It can be placed as sister to Paragonimus in some analyses and falls within the genus in others. A recently published morphological study placed E. cenocopiosus within the genus Paragonimus and probably this is where it should remain.
  Journal of Helminthology 11/1999; 73(04):295 - 299. · 1.38 Impact Factor