Tue Nguyen

Publications (2)5.99 Total impact

• Article: Periodontal repair in dogs: effect of recombinant human transfornning growth factor‐β1 on guided tissue regeneration

  Ulf M. E. Wikesjö, Saghi S. Razi, Thorarinn J. Sigurdsson, Dimitris N. Tatakis, Michael B. Lee, Boonsri Ongpipattanakul, Tue Nguyen, Ross Hardwick
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  ABSTRACT: This study evaluated alveolar bone and cementum regeneration following surgical implantation of recombinant human transforming growth factor-β1 (rhTGF-β1) in conjunction with guided tissue regeneration (GTR). Supraalvcolar, critical size, periodontal defects were surgically created around the 3rd and 4th mandibular premolar teeth in right and left jaw quadrants in 5 beagle dogs. Alternate jaw quadrants in consecutive animals received rhTGF-β1 in a CaCO3/ hydroxyethyl starch carrier with GTR, or carrier with GTR alone (control), 20μg of rhTGF-/A in buffer solution was incorporated into approximately 0.8 ml of carrier for each defect scheduled to receive rhTGF-β1. Animals were sacrificed at week 4 postsurgery and tissue blocks were harvested and processed for histo-metric analysis. Clinical healing was generally uneventful. Minor membrane exposures were observed. Defects with membrane exposure displayed an inflammatory infiltrate underneath the membrane. Bone regeneration of trabecular nature, apparent in all animals, was generally limited to the very apical aspect of the defects. Cementum regeneration was limited without obvious differences between experimental conditions. Comparing rhTGF-β to control defects, statistically significant differences were found for area (1.8±0.4 and 1.3±0.6 mm2, respectively: p<0.05) and density (0.3±0.1 and 0.2±0.03. respectively: p<0.05) of alveolar bone regeneration. Observed differences are small and represent a clinically insignificant potential for enhanced regeneration in this preclinieal model. Within the limitations of study, it may be concluded that rhTGF-β1 has a restricted potential to enhance alveolar bone regeneration in conjunction with GTR.
  Journal Of Clinical Periodontology 12/2005; 25(6):475 - 481. · 3.00 Impact Factor
• Article: Periodontal repair in dogs: effect of transforming growth factor‐β1 on alveolar bone and cementum regeneration

  Dimitris N. Tatakis, Ulf M. E. Wikesjö, Saghi S. Razi, Thorarinn J. Sigurdsson, Michael B. Lee, Tue Nguyen, Boonsri Ongpipattanakul, Ross Hardwick
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  ABSTRACT: Background, aim: Transforming growth factor-β (TGF-β) represents a family of growth-modulating proteins with fundamental rôles in connective tissue and bone development. The objective of this study was to evaluate the potential for regeneration of alveolar bone and cementum following surgical implantation of recombinant human TGF-β1 (rhTGF-β1).Method: Bilateral, critical size, supra-alveolar periodontal defects in 5 beagle dogs were surgically implanted with rhTGF-β1 in a calcium carbonate carrier (CaCO3) or with carrier alone. The animals were euthanized at 4 weeks post-surgery and block-biopsies of the defects were processed for histologic and histometric analysis.Results: Surgical implantation of rhTGF-β1 resulted in minimal, if any, stimulation of alveolar bone or cementum regeneration. Linear bone and cementum regeneration in rhTGF-β1-treated defects was 1.2±0.6 and 0.01±0.01 mm, respectively. Correspondiong values for the controls were 1.0±0.6 and 0.01±0.03 mm.Conclusions: The results suggest that, under the conditions (dose, carrier, defect type) evaluated here, treatment of periodontal defects in beagle dogs with rhTGF-β1 may be of limited clinical benefit.
  Journal Of Clinical Periodontology 12/2001; 27(9):698 - 704. · 3.00 Impact Factor