Yuan Yue

Hong Kong SAR Government, Hong Kong, Hong Kong

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Publications (9)18.93 Total impact

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    ABSTRACT: It has been widely reported that periodontitis may lead to bone tissue and teeth loss and result in failure of prosthodontics or implants. Interleukin-1 (IL-1) is a potent proinflammatory cytokine that plays an essential role during the pathogenesis of periodontitis. However, the gene polymorphisms of IL-1α, IL-1β and IL-1RN and the relationship between these protein expressions in healthy people and patients with chronic periodontitis (CP) in China have not been fully elucidated. We investigated the gene polymorphisms and protein expression of IL-1α, IL-1β and IL-1RN in healthy subjects and CP patients, and our data suggest that these gene polymorphisms are associated with CP. The frequency of the C/C genotype of IL-1α was 55% in CP patients, while in the control group it was 20% (p<0.0001). The C/C genotype of IL-1β was also higher in CP patients (51%) than in controls (21%) (p<0.0001). For the 2/2 genotype of IL-1RN, CP patients showed a 30% frequency, while in controls this was 15% (p<0.0001). Protein levels evaluated by enzyme-linked immunosorbent assay demonstrated a significant difference in secretion between patients and controls for IL-1α and IL-1β. These results indicate that genotype and protein production of IL-1α, IL-1β and IL-1RN are associated with CP in a Chinese population, and might be putative risk indicators for chronic periodontitis.
    The International journal of biological markers 04/2013; 28(1). DOI:10.5301/jbm.5000013 · 1.36 Impact Factor
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    ABSTRACT: Objectives: This study aims to evaluate and compare cytokines in gingival crevicular fluid (GCF) and saliva of patients with aggressive periodontitis (AP) before and after treatment. Methods: Forty AP patients and 40 healthy volunteers were enrolled in this study. Clinical parameters included probing depth and sulcus bleeding index. GCF and saliva were collected from both groups. The levels of IL-1β, IL-2, IL-4, IL-6, IFN-γ and TNF-α were measured using ELISA. Results: The probing depth in AP patients was significantly deeper before treatment than after treatment. The concentrations of cytokines in GCF and saliva were significantly higher in AP patients than in the control group and decreased after periodontal treatment. Positive relationships were found between cytokine levels in GCF and clinical parameters. The reliability of cytokines in GCF and saliva was assessed by Cronbach's alpha analysis, which could be considered satisfactory. Conclusion: Cytokine levels in GCF and saliva correlated well with clinical parameters and AP. Measurements of cytokines in saliva may be regarded as a noninvasive and quick method for monitoring periodontal disease activity.
    The International journal of biological markers 04/2013; DOI:10.5301/jbm.5000014 · 1.36 Impact Factor
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    ABSTRACT: Purposes: This study aimed at investigating the association between interleukin-6 (IL-6), interleukin-12 (IL-12), C-reactive protein (CRP), vascular endothelial growth factor (VEGF) and β-defensin-1<sup> </sup>polymorphisms and the susceptibility to periodontitis in the Chinese population. Methods: DNA was extracted from the blood samples of 532 healthy individuals and 122 chronic periodontitis (CP) patients enrolled in the study. The genes encoding IL-6, IL-12, CRP, VEGF and β-defensin-1 were amplified using PCR and digested with restriction enzymes. The protein expression of the abovementioned genes was determined by ELISA. Differences in the allele/genotype frequencies were assessed with the chi-square test. Results: The frequencies of the C/C genotypes of IL-6, IL-12, and VEGF were higher in CP patients than healthy controls (66.3% vs 25.9%; 27.8% vs 19.9%; and 64.8% vs 52.1%, respectively). In the patients' group we also recorded frequencies of the A/A genotypes of CRP and VEGF higher than in healthy controls (63.1% vs 58.1% and 64.8% vs 35.2%, respectively). Protein production evaluated by ELISA demonstrated significant differences between CP patients and healthy controls for IL-6, IL-12, CRP, VEGF and β-defensin-1. Conclusions: The genotypes of IL-6, IL-12, VEGF and β-defensin-1 and their protein productions were associated with CP in a Chinese population. Genotypes and serum levels of CRP were associated with CP, but alleles frequency showed no difference between CP patients and healthy controls.
    The International journal of biological markers 04/2013; 28(1). DOI:10.5301/jbm.5000010 · 1.36 Impact Factor
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    ABSTRACT: Objectives: Diminished bone mineral density (BMD) and micro-circulation often occur in pre-menopausal women who consume anti-estrogenic activity drugs on a regular basis affecting osseointegration of dental implants. Estrogen receptor positive breast cancer patients are mainly treated by Tamoxifen which reduces bone density and micro-circulation orally affecting the stability of implants. In this study, 17 hormone-positive (HP) and 10 hormone-negative (HN) invasive breast cancer pre-menopausal patients were observed, age range between 28 and 40. Methods: The selected subjects had at least one implant in the anterior region (total 37 implants) 12-24 months before cancer diagnosis. After modified mastectomy operation, they received 4 cycles of 500mg/m2 5-fluorouracil, 75 mg/m2 epirubicin and 500mg/m2 cyclophosphamide. The HP group continually accepted 20mg Tamoxifen daily for 5 years. Both groups were monitored every 6 months systemically and dentally. The micro-circulation was monitored capillaroscopically in lingual mucosa and BMD changes were examined by dual-energy-X-ray-absorptiometry (DEXA). Results: No recurrent and distant metastasis were observed 36 months after 4 cycles of chemotherapy but Pearson chi-square test showed that HP group had a higher failure rate (44%, 12/27) than HN group (30%, 3/10) (p=0.04). Capillaroscopy monitored the capillary loop length, loop diameter and capillary density These mean values were lower in HP than HN group with significant difference (p<0.001). Independent Test demonstrated the mean T-score of BMD in HP group was -1.88±0.36 which was lower than HN group -1.37±0.52 (p=0.006). Conclusions: Capillaroscopy is a non-invasive and sensitive technique, it can be used in the diagnosis and monitoring of microangiopathies. Long term administration of anti-estrogenic activity drugs suppresses hormonal level causing poor microcirculation in the oral mucosa and lower bone density in the alveolar ridge. As a result the osseointegration of dental implants may become unstable and fail eventually.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
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    ABSTRACT: Objectives: The survival of avulsed permanent teeth is affected by regeneration of periodontal ligament (PDL), development of root resorption, ankylosis. Human adipose-tissue-derived stem cells (HADSCs) and platelet-rich plasma (PRP) have been reported to contribute to wound healing and tissue regeneration. In this study we mixed HADSCs with PRP, applying to the roots of avulsed incisors before replantation. Methods: 52 avulsed central incisors were collected from 36 males and 16 females (age 14-20), they were stored and transported in milk to dental clinic. Root canal treatment was conducted in vitro, the teeth were immersed in physiological saline with antibiotics. The PRP was obtained from each subject by an automated platelet concentration system. HADSCs had been isolated from healthy females’adipose-tissue and identified with stem cell biomarkers. The cells were seeded into sterile collagen sponge mixed with prepared PRP. The roots of avulsed incisors were covered with mixed gel and inserted into prepared alveolar socket, the teeth were fastened to adjacent teeth by a splint. The tooth was monitored clinically and radiologically at 1, 3, 6, 12, 18, 24 and 36 months after replantation. Results: The radiological examination at 36 months revealed 40 teeth (77%) with PDL healing, 7(13%) developed root ankylosis, 3(6%) with root resorption and 2(4%) failed after 12-18 months. The existing 50 teeth demonstrated natural color of appearance. 90% of the teeth showed MI mobility, absence of gingival recession, clinical attachment loss and no sites with probing depth >3mm. Successful regeneration of avulsed teeth with PDL healing was shown to be significantly higher than other forms of replantation. Conclusions: Mixed HADSCs and PRP applied to the roots may fill the gap of avulsed teeth, inducing PDL healing and reducing the chance of root resorption and ankylosis. Preparation of HADSCs and PRP are easy and safe, it can be widely used in replantation and transplantation.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
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    ABSTRACT: Periodontal disease is thought to arise from the interaction of various factors, including the susceptibility of the host, the presence of pathogenic organisms, and the absence of beneficial species. The genetic factors may play a significant role in the risk of periodontal diseases. Cytokines initiate, mediate and control immune and inflammatory responses. The aim of this study is to compare genotypes and soluble protein of pro and anti-inflammatory cytokines (IL-1α, IL-1β, IL-6, IFN-γ, IL-10, TNF-α and IL-4) in subjects with or free of chronic periodontitis. A total of 1,290 Chinese subjects were recruited to this clinical trial: 850 periodontally healthy controls and 440 periodontal patients. All subjects were free of systemic diseases. Oral examinations were performed, and the following parameters were recorded for each subject: supragingival/subgingival calculus, gingival recession, bleeding on probing (BOP), probing depth (PD), clinical attachment loss (CAL), gingival recession and tooth mobility. The peripheral blood samples were collected for genetic and enzyme linked immunosorbent assay (ELISA) analysis. Restriction enzymes were used for digestion of amplified fragments of IL-1α, IL-1β, IL-6, IFN-γ, IL-10, TNF-α and IL-4. The protein expressions of patient and control samples for IL-1α, IL-1β, IL-6, TNF-α, IFN-γ, IL-10, and IL-4 measured by ELISA confirmed a statistically significant difference (p < 0.001). The digestion of fragments of various genes showed that the pro-inflammatory cytokines IL-1α and TNF-α, and the anti-inflammatory cytokines IL-4 and IL-10 demonstrated a correlation with chronic inflammation in patients (X2: p < 0.001). The remaining genes investigated in patients and healthy subjects (IL-1β, IL-6, IFN-γ and IL-10) did not show any significant difference. The cytokine gene polymorphisms may be used as a marker for periodontitis susceptibility, clinical behaviour and severity. This detection offers early diagnosis and induction of prophylaxis to other family members against disease progression.
    Journal of Translational Medicine 09/2012; 10 Suppl 1(Suppl 1):S8. DOI:10.1186/1479-5876-10-S1-S8 · 3.99 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: Periodontitis is a common disease that affects the periodontal tissue supporting the teeth. This disease is attributed to multiple risk factors, including diabetes, cigarette smoking, alcohol, pathogenic microorganisms, genetics and others. Human beta-defensin-1 (hBD-1) is a cationic antimicrobial peptide with cysteine-rich ß-sheets and broad-spectrum antimicrobial activity. CD14 is a protein involved in the detection of bacterial lipopolysaccharide (LPS) and has also been associated with periodontitis. This study investigates the single nucleotide polymorphic (SNP) region, -1654(V38I), of the human beta-defensin-1 (hBD-1) gene as well as the -159 region of the CD14 gene in subjects with chronic periodontitis. Blood samples from periodontally healthy subjects and periodontitis patients were obtained. DNA was extracted from the blood and was used to perform restriction digest at the polymorphic G1654A site of DEFB1 with the enzyme HincII. The polymorphic site 159TT of CD14 was digested with the enzyme AvaII. Enzyme-linked immunosorbent assay (ELISA) was performed on soluble samples to determine the protein expressions. The control and patient groups expressed 35% and 38% 1654 A/A genotype of DEFB1, respectively. The A allele frequency of the control group was 40%, while the patient blood group was 54%. The mean hBD-1 protein levels of the control and patient samples were 102.83 pg/mL and 252.09 pg/mL, respectively. The genotype distribution of CD14 in healthy subjects was 16% for C/C, 26% for T/T and 58% for C/T. The genotype frequencies of CD14 in periodontitis patients were 10% for C/C, 43% for T/T and 47% for C/T. The CD14 protein expression determined by ELISA showed a mean protein level of the control samples at 76.28ng/mL and the patient blood samples at 179.27ng/mL with a p value of 0.001.Our study demonstrated that patients suffering from chronic periodontitis present more commonly with the 1654A/A genotype on the DEFB1 gene and the 159T/T genotype on the CD14 gene. This study purely investigated the association between periodontitis and one polymorphic site on both DEFB1 and CD14 gene, with the purpose of expanding knowledge for the future development in diagnostic markers or therapeutic interventions to combat this disease.
    Journal of Translational Medicine 09/2012; 10 Suppl 1(Suppl 1):S9. DOI:10.1186/1479-5876-10-S1-S9 · 3.99 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: Chronic periodontitis (CP) is a chronic inflammation associated with elevations of several inflammatory and cardiac markers. Studies implicated CP as one of the etiologies in coronary heart disease (CHD). Cardiotoxicity is a major complication of anticancer drugs, including anthracyclines and 5-fluorouracil (5FU). The most severe cardiac complications are heart failure, arrhythmia and coronary heart disease (CHD). In this study, we compared the level of inflammatory factors and cardiac markers between chronic periodontitis patients and cancer patients receiving chemotherapy. 108 blood samples of periodontally healthy subjects were obtained on random from Hong Kong Red Cross, and these represented the controlled population. Forty-four patients diagnosed with chronic periodontitis were recruited from the West China Hospital of Stomatology, Sichuan University. They have received scaling and root planning with mean pocket depths of 6.05 mm. Thirty breast cancer patients diagnosed with invasive ductal carcinoma from UNIMED Medical Institute, Hong Kong gave consent to participate in this study. They received 4 cycles of 500mg/m2 5-fluorouracil, 75 mg/m2 epirubicin and 500mg/m2 cyclophosphamide at a 3-week interval between each cycle. Peripheral venous blood from each group was taken for measurement of blood cells, inflammatory marker (P-selectin, high sensitvity C-reactive protein) and cardiac markers (troponin T; troponin I; N-terminal pro brain natriuretic peptide (Nt-proBNP) and Lactate dehydrogenase (LDH). The lymphocyte count was higher (p < 0.05) in periodontitis patients than the other two groups, and more neutrophils (p < 0.05) were seen in cancer patients receiving chemotherapy. The two test groups demonstrated higher levels (p < 0.01) of inflammatory and cardiac markers than the control group. The elevated cardiac markers found in periodontitis patients suggested that they may carry potential risks in developing cardiac lesions. Troponin T, troponin I, pro-BNP, LDH and high sensitvity C-reactive protein may be used as markers to monitor cardiac lesions in chronic inflammatory patients.
    Journal of Translational Medicine 09/2012; 10 Suppl 1(Suppl 1):S5. DOI:10.1186/1479-5876-10-S1-S5 · 3.99 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: Chronic periodontitis (CPs) could result in damage of periodontal tissues, loss of teeth and impose troublesome hindrance to restore teeth satisfyingly as well. Functional gene polymorphisms of matrix metalloproteinases, cytokines and cyclooxygenase-2 have been found to play important roles in periodontitis. This study was to investigate the association between MMP-1-1067, MMP-3-1171, MMP-9-1562, IL-2-330, IL-8-251, COX-2-765 polymorphisms, and the susceptibility to CP in a Chinese population. A total of 122 patients with CP were evaluated for MMP-1, MMP-3, MMP-9, IL-2, IL-8 and COX-2 genetic polymorphisms and were compared with 532 healthy control subjects using PCR-RFLP analysis. Clinical periodontal parameters were recorded. Serum levels of MMP-1, MMP-3, MMP-9, IL-2, IL-8 and COX-2 were measured by ELISA. The data were analyzed by chi-square, logistic regression and Mann-Whitney-U-tests and t test. There were significant differences between CP patients and healthy subjects in the genotype distribution and allele frequency of MMP-3-1171, MMP-9-1562, IL-2-330, IL-8-251 and COX-2-765 genetic polymorphisms. Significant difference between patients and controls were also observed for MMP-1-1067 genotype frequency, but not for allele frequency. Differences between rare allele carriage rates of CP and healthy groups regarding all the genetic polymorphisms in our study were significant (p<0.05). Serum levels of all the cytokines were higher in the CP patients compared to healthy subjects. These data show that MMP-1-1067, MMP-3-1171, MMP-9-1562 and IL-8-251 polymorphisms are associated with susceptibility to CP. MMP-1-1067 2G, MMP-3-1171 6A, MMP-9-1562 T and IL-8-251 A allele are associated with decreased susceptibility to CP in Chinese population.
    Cytokine 07/2012; 60(2):552-60. DOI:10.1016/j.cyto.2012.06.239 · 2.87 Impact Factor

Publication Stats

21 Citations
18.93 Total Impact Points


  • 2012
    • Hong Kong SAR Government
      Hong Kong, Hong Kong
    • Sichuan University
      • Department of Prosthodontics
      Hua-yang, Sichuan, China
    • University of Kragujevac
      Krabujevac, Central Serbia, Serbia