[Show abstract][Hide abstract] ABSTRACT: To determine the predictive accuracy of the combined panels of serum human tissue kallikreins (hKs) and CA-125 for the detection of epithelial ovarian cancer.
Serum specimens collected from 5 Indonesian centers and 1 Vietnamese center were analyzed for CA-125, hK6, and hK10 levels. A total of 375 specimens from patients presenting with ovarian tumors, which include 156 benign cysts, 172 epithelial ovarian cancers (stage I/II, n=72; stage III/IV, n=100), 36 germ cell tumors and 11 borderline tumors, were included in the study analysis. Receiver operating characteristic analysis were performed to determine the cutoffs for age, CA-125, hK6, and hK10. Sensitivity, specificity, negative, and positive predictive values were determined for various combinations of the biomarkers.
The levels of hK6 and hK10 were significantly elevated in ovarian cancer cases compared to benign cysts. Combination of 3 markers, age/CA-125/hk6 or CA-125/hk6/hk10, showed improved specificity (100%) and positive predictive value (100%) for prediction of ovarian cancer, when compared to the performance of single markers having 80-92% specificity and 74-87% positive predictive value. Four-marker combination, age/CA-125/hK6/hK10 also showed 100% specificity and 100% positive predictive value, although it demonstrated low sensitivity (11.9%) and negative predictive value (52.8%).
The combination of human tissue kallikreins and CA-125 showed potential for improving prediction of epithelial ovarian cancer in patients presenting with ovarian tumors.
[Show abstract][Hide abstract] ABSTRACT: Extraction of genomic DNA is the very first experiment in the study cascade of human inherited diseases. Blood is preferred to be the material for DNA extraction. In the case of limitation of the material source, for example, new born screening program, blood is saved as dried spot in filter paper. In practical, long term storing blood sample as the dried spots on filter paper leads to more advantages for DNA extraction essay which need to be performed several times. This study provides an efficient method with 2 step lyses allow high concentration of DNA is extracted from samples. Comparing to the commercial QIAamp® DNA Mini Kit DNA concentration extracted from this method is higher, the quality of DNA is equal and the cost is lower. The optimal DNA extraction method based on phenol in this study is useful tool which may apply for further molecular analysis studies using dried blood spot samples.