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ABSTRACT: To evaluate the quality of Polygonum perfoliatum collected from GAP planting base efficiently so as to provide scientific evidence for its GAP tending plant by HPLC fingerprinting method. Analysis was performed on a reserved-phase column Lichrosher-C18 column (4.6 mm x 250 mm, 5 microm), A linear gradient elution of 0.2% aqueous acetic acid and acetonitrile was used for the separation. Wildlife tending P. perfoliatum was relatively stable. The chromatograms of the samples from 2011 were more accordant than those from 2010. Samples in Dafang were the most stable and had little differences during the two years. The quality of P. perfoliatum planted in the regions of sample GBG-(GD) 002 in Guiding and sample GBG-(LL) 003 in Longli were more stable and better than those from other regions. It is a feasible method that can be used to evaluate the quality of P. perfoliatum for GAP planting.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 05/2012; 37(9):1180-3.