RenéT. Wintjens

Free University of Brussels, Brussels, BRU, Belgium

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Publications (1)4.17 Total impact

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    ABSTRACT: Papain is widely used in several laboratories as a reference enzyme and, as such, needs to be highly pure. A one-step purification of the enzyme is described here starting from spray-dried papaya latex. After protection of the essential SH group with a monomethoxypolyethylene glycol derivative synthesised in our laboratory, the mixture of papaya enzymes is submitted to fractionation on S-Sepharose Fast Flow. This procedure leads to the isolation of the S-pegylthiopapain conjugate devoid of any contamination nor by the enzyme's irreversibly oxidized form nor by one of the other proteinases present in the original mixture. Thereafter, S-pegylthiopapain may be quite easily converted into its fully active form.
    Journal of Chromatography A 02/1996; 724(1-2):185-192. DOI:10.1016/0021-9673(95)00910-8 · 4.17 Impact Factor

Publication Stats

12 Citations
4.17 Total Impact Points

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  • 1996
    • Free University of Brussels
      • Protein Chemistry (SPRO)
      Brussels, BRU, Belgium