Publications (2)2.51 Total impact
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ABSTRACT: Chokecherry (Prunus virginiana L.) (2n = 4x = 32) is a unique Prunus species for both genetics and disease-resistance research due to its tetraploid nature and X-disease resistance. However, no genetic and genomic information on chokecherry is available. A partial chokecherry genome was sequenced using Roche 454 sequencing technology. A total of 145,094 reads covering 4.8 Mbp of the chokecherry genome were generated and 15,113 contigs were assembled, of which 11,675 contigs were larger than 100 bp in size. A total of 481 SSR loci were identified from 234 (out of 11,675) contigs and 246 polymerase chain reaction (PCR) primer pairs were designed. Of 246 primers, 212 (86.2 %) effectively produced amplification from the genomic DNA of chokecherry. All 212 amplifiable chokecherry primers were used to amplify genomic DNA from 11 other rosaceous species (sour cherry, sweet cherry, black cherry, peach, apricot, plum, apple, crabapple, pear, juneberry, and raspberry). Thus, chokecherry SSR primers can be transferable across Prunus species and other rosaceous species. An average of 63.2 and 58.7 % of amplifiable chokecherry primers amplified DNA from cherry and other Prunus species, respectively, while 47.2 % of amplifiable chokecherry primers amplified DNA from other rosaceous species. Using random genome sequence data generated from next-generation sequencing technology to identify microsatellite loci appears to be rapid and cost-efficient, particularly for species with no sequence information available. Sequence information and confirmed transferability of the identified chokecherry SSRs among species will be valuable for genetic research in Prunus and other rosaceous species. Key message A total of 246 SSR primers were identified from chokecherry genome sequences. Of which, 212 were confirmed amplifiable both in chokecherry and other 11 other rosaceous species.Plant Cell Reports 07/2012; 31(11):2047-55. · 2.51 Impact Factor
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ABSTRACT: To direct, fund, promote and communicate horticultural research, which increases the quality and value of ornamental plants, improves the productivity and profitability of the nursery and landscape industry, and protects and enhances the environment. The use of any trade name in this article does not imply an endorsement of the equipment, product or process named, nor any criticism of any similar products that are not mentioned. Abstract An effective plant regeneration system was developed for chokecherry (Prunus virginiana L.) by using in vitro leaf tissues. Adventitious shoots regenerated from in vitro leaf tissues only when cultured on Woody Plant Medium (WPM), but not on Murashige and Skoog medium, supplemented with benzyladenine (BA) or thidiazuron (TDZ). Three chokecherry clones (NN, 10, and 17) responded differently to types and concentrations of cytokinins, ranging from 16.7 to 91.7% leaf explants regenerating shoots. A mean of four shoots was produced from each explant, with the most shoots (> 10) from clone NN on media with 5–10 µM BA. Higher concentrations of TDZ (> 8 µM) caused serious vitrification and eventual death of newly induced shoots. Regenerated shoots (> 1.5 cm) produced roots in vitro in half strength MS medium or ex vitro in Cellular Rooting Sponge (CRS) rooting plugs with or without auxin (NAA or IBA) treatments. Rooting was affected by auxin, genotypes, and the rooting methods.J. Environ. Hort. 01/2005; 22:225-228.