ABSTRACT: Sera from 174 equidaes (15 mules and 161 equines) of Monte Negro municipality, Rondônia State were analyzed against viral and bacterial agents. The serum sample corresponded the total equid population in the municipality considering a confidence interval of 99%, expected prevalence of 50% and absolute desired of 10%. For the viral agents, sera were tested by the Agar Gel Immunodiffusion Test (Equine
Infection Anemia - EIA), Inhibition Haemagglutination Test (Equine Influenza 1 and 2 – EI–1 and 2), and Virusneutralizating Tests (Equine Viral Arteritis – EVA, Equine Herpesvirus 1 – EHV1, Vesicular Stomatitis – VS, Equine Encephalitis Eastern - EEE, Western - WEE and Venezuelan VEE). The diagnosis for brucellosis was made by Agglutination Tests and the Microscopic Agglutination Test was used for leptospirosis. The results showed positivity of 9.6% for EIA, 22.7% for HVE1, 19.9% for IE-1, 42.0% for IE-2,
21.0% for EEE, 11.3% for VEE, 3.4% for brucellosis, and 91.4% for leptospirosis. The most frequent serovars detected were Bratislava (10.5%), Icterohaemorrhagiae (8.7%), Autumnalis (8.7%) for equines
and Patoc (26.6%) for mules. No one of the examined samples reacted to EVA, VS, or WEE.
Brazilian Journal of Veterinary Research and Animal Science 01/2008; 45(4):269-276.