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ABSTRACT: Periodontal ligament (PDL) can be obtained from patients undergoing orthodontic treatment. PDL contains progenitor cells that can be expanded and differentiated towards several mesenchymal lineages in vitro. Furthermore, PDL-derived cells have been shown to generate bone- and PDL-like structures in vivo. Thus, PDL cells, combined with suitable biomaterials, represent a promising tool for periodontitis-related research and PDL engineering. Here, a new PDL cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was created. HTERT-expressing PDL cells showed similar morphology and population doubling time but an extended lifespan compared to the primary cells. In addition, PDL-hTERT cells expressed several characteristic genes and upon osteogenic stimulation produced a calcified matrix in vitro. When cultivated on two topographically different titanium scaffolds (MA and SLA), PDL-hTERT cells exhibited augmented spreading, survival and differentiation on smooth (MA) compared to rough (SLA) surfaces. These findings differ from previously reported osteoblast behaviour, but they are in agreement with the behaviour of chondrocytes and gingival fibroblasts, suggesting a very cell type-specific response to different surface textures. In summary, we report the testing of titanium biomaterials using a new PDL-hTERT cell line and propose this cell line as a useful model system for periodontitis research and development of novel strategies for PDL engineering.
European cells & materials 01/2010; 19:228-41. · 3.03 Impact Factor
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ABSTRACT: Prosthetic crowns made by galvano-forming are considered to be highly biocompatible and aesthetic restorations. Therefore, they represent an alternative crown-system to conventional metal-ceramic crowns in replacing lost tooth structure. However, there are few data available on clinical and biochemical effects of galvano-ceramic crowns on periodontal tissues. The purpose of this controlled study was to test the impact of galvano-ceramic crowns and metal-ceramic crowns on clinical and inflammatory responses of periodontal tissues. A prospective, blinded randomized clinical trial was conducted. Galvano-ceramic crowns and metal-ceramic crowns were placed in 52 periodontally healthy patients in split-mouth design. Clinical parameters (gingival index, plaque index, probing depths and recessions) were recorded from six sites per tooth. Initial tissue alteration was accessed analysing the gingival crevicular fluid flow rate and IgG concentration in gingival crevicular fluid. After 24 months, 34 patients could be re-evaluated. All crowns were in adequate function and obvious clinical inflammation was rarely observed. After 24 months of follow-up, gingival tissues adjacent to galvano-ceramic crowns showed significantly less signs of clinical and inflammatory responses according to plaque index (P = 0.004), gingival index (P < 0.001), gingival crevicular fluid flow rate (P = 0.012) and IgG (P = 0.002). Data were also analyzed for buccal and oral sites separately. Gingival tissues adjacent to metal-ceramic crowns showed significantly increased clinical and inflammatory values for plaque index (P = 0.005), gingival index (P = 0.008), gingival crevicular fluid flow rate (P = 0.006), IgG (P = 0.007) at oral sites compared to galvano-ceramic crowns. Our data suggest a stabilizing effect of galvano-ceramic crowns on periodontal tissues over time.
Journal of Oral Rehabilitation 12/2007; 34(12):941-7. · 1.53 Impact Factor
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Sportverletzung · Sportschaden 07/2002; 16(2):59-63. · 0.61 Impact Factor
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ABSTRACT: Although golf is perceived as a sport with low risk for injury, majority of golf players complain about problems along the spine. One possible reason could be poor physical condition. To see if golf players in general show deficits in spine-stabilizing muscles, twenty-three male golfers without back pain were examined with regard to their maximal isometric strength of trunk muscles. They were compared with persons of same age and constitution that do no sports. The Golf players have highly significant stronger lumbar extensors and show muscular dysbalance in lateral flexors and rotators of the spine. Specific training for well-balanced strengthening of spine-stabilizing muscles seem to be advisable, because muscular dysbalance can contribute to the development of back pain.
Sportverletzung · Sportschaden 07/2000; 14(2):55-8. · 0.61 Impact Factor
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Sportverletzung · Sportschaden 04/1999; 13(1):M7-9. · 0.61 Impact Factor
