Chinese Journal of Veterinary Science and Technology. 33(10):55-57.
ABSTRACT: 閲囩敤RT-PCR娉曞FMDV OX鏍熀鍥犵粍鍏ㄥ簭鍒楄繘琛屼簡鍒嗗瓙鍏嬮殕涓庢祴搴?缁撴灉琛ㄦ槑涓嶅惈poly(C)搴忓垪鍜宲oly(A)灏惧反鐨凮X鏍熀鍥犵粍鍏ㄥ簭鍒楅暱8 104nt,寮€鏀捐鐮佹(ORF)闀? 969nt銆佺紪鐮? 322aa,5鈥睻TR闀? 040nt,3鈥睻TR闀?5nt.搴旂敤鍒嗗瓙鐢熺墿瀛﹁蒋浠跺皢OX鏍笌FMDV鍏跺畠鍙傝€冩瘨鏍繘琛屽簭鍒楁瘮杈?骞跺鍏跺熀鍥犵壒寰佽繘琛屽垎鏋?缁撴灉鏄剧ず,OX鏍笌OTw yl/97鐨勬牳鑻烽吀鍚屾簮鎬ф渶楂?鍦ㄥ熀鍥犵粍鍔熻兘鏈煡鍖哄拰3A缂栫爜鍖哄叿鏈変袱澶勬槑鏄剧殑缂哄け鐜拌薄,鍏朵竴鏄湪3A缂栫爜鍖烘湁30nt鐨勮繛缁己澶?杩欎笌OTwyl/97 鏍浉鍚?鍏朵簩鏄湪鍔熻兘鏈煡鍖哄煙鏈?4nt鐨勭己澶?杩欎笌OTwyl/97鏍浉鍚?涓嶰Akesu58鐩镐技(43nt缂哄け).
The complete sequence of foot-and-mouth disease virus (FMDV) OX strain was acquired by RT-PCR. Then all regions of OX strain genome were compared with those of the reference strains which sequences were obtained from GenBank > and their genetic characteristic were analyzed by molecular biological software. As a result, the genome of FMDV OX strain is 8 104nt without poly(C) and poly (A), and is composed of 5'untranslated region (UTR), open-reading frame (ORF) and 3'untranslated region (UTR), their nucleotide is 1 040nt, 6 969nt and 95nt respectively. The OX strain has the most homologous with OTWyl/97 and there are two distinct genetic deletions in function-unknown region and 3A encoding region, one is a 30nt sequential deletion in 3A encoding region which is same as OTWyl/97 strain, the other is a 44nt sequential deletion in unknown-functional region which is same as OTWyl/97 strain and similar to OAkesu58 (43nt deletion) strain.
Acta Veterinaria et Zootechnica Sinica. 35(4):454-458.
Chinese Journal of Veterinary Science and Technology. 33(9):3-6.