[Show abstract][Hide abstract] ABSTRACT:
The complete sequence of foot-and-mouth disease virus (FMDV) OX strain was acquired by RT-PCR. Then all regions of OX strain genome were compared with those of the reference strains which sequences were obtained from GenBank > and their genetic characteristic were analyzed by molecular biological software. As a result, the genome of FMDV OX strain is 8 104nt without poly(C) and poly (A), and is composed of 5'untranslated region (UTR), open-reading frame (ORF) and 3'untranslated region (UTR), their nucleotide is 1 040nt, 6 969nt and 95nt respectively. The OX strain has the most homologous with OTWyl/97 and there are two distinct genetic deletions in function-unknown region and 3A encoding region, one is a 30nt sequential deletion in 3A encoding region which is same as OTWyl/97 strain, the other is a 44nt sequential deletion in unknown-functional region which is same as OTWyl/97 strain and similar to OAkesu58 (43nt deletion) strain. 104nt,开放读码框(ORF)长6 969nt、编码2 322aa,5′UTR长1 040nt,3′UTR长95nt.应用分子生物学软件将OX株与FMDV其它参考毒株进行序列比较,并对其基因特征进行分析.结果显示,OX株与OTw yl/97的核苷酸同源性最高,在基因组功能未知区和3A编码区具有两处明显的缺失现象,其一是在3A编码区有30nt的连续缺失,这与OTwyl/97 株相同,其二是在功能未知区域有44nt的缺失,这与OTwyl/97株相同,与OAkesu58相似(43nt缺失).