Tatiane T.N. Watanabe

Universidade Federal do Rio Grande do Sul, Pôrto de São Francisco dos Casaes, Rio Grande do Sul, Brazil

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Publications (9)6.45 Total impact

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    ABSTRACT: Actinobacillosis is a not contagious infectious disease, usually chronic, and characterized by pyogranulomatous inflammatory reaction that occurs in cattle and is less common in sheep, pig, and horse. Soft tissues of head, mouth, and regional lymph nodes are affected. In this retrospective study, lesions suggestive of Actinobacillosis from 18 cases of anatomopathologic bovine samples were retrieved from the archives of Setor de Patologia Veterinária of Universidade Federal do Rio Grande do Sul, from January 1997 to May 2011. The lesions were classified histologically, evaluated by histochemistry, and immunohistochemistry (IHC) in order to standardize inflammatory infiltration. The most frequent histological findings were typical pyogranulomas of actinobacillosis contained radiating eosinophilic clubs surrounded by neutrophils, macrophages, lymphocytes and abundant fibrous connective tissue. Gram-negative coccobacillus bacteria were shown in all lesions by Gram stain. Abundant macrophages were immunopositive for CD68, especially epithelioid cells and multinucleated giant cells. In addition, a positive immunostaining for CD3 (T lymphocyte) was observed in proportion of 1:3 in lymphocyte inflammatory infiltration, while IHC for CD79αcy (B lymphocytes) was obtained in proportion of 2:3. These results indicated that B lymphocytes are the majority of lymphocyte in the inflammatory area.
    Pesquisa Veterinária Brasileira 03/2013; 33(3):305-309. · 0.54 Impact Factor
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    ABSTRACT: São descritos surtos de intoxicação espontânea por Cestrum intermedium em bovinos leiteiros do Município de Salgado Filho, sudoeste do Estado do Paraná, com morte de dezesseis bovinos de um total de 72 de quatro pequenas propriedades. O histórico obtido nessas propriedades incluía curso clínico agudo, com morte em menos de 24 horas. Em todas foram observadas elevada lotação por área com escassa pastagem disponível e grande quantidade de plantas arbustivas identificadas como C. intermedium e com sinais de terem sido consumidas pelos animais. Dois bovinos foram necropsiados e os achados mais importantes foram congestão de mucosas conjuntivais e vulvovestibular; hemorragias e evidenciação do padrão lobular no fígado; hemorragia, congestão e edema pulmonares; congestão esplênica e encefálica, além de hemorragias multifocais no coração. Amostras de tecidos desses bovinos foram avaliadas, com alterações histológicas importantes no fígado, caracterizadas por necrose acentuada de hepatócitos e hemorragia, indicativas de hepatopatia tóxica aguda e, no encéfalo, foram observadas alterações astrocitárias em substância branca de cérebro e cerebelo e na substância cinzenta do cerebelo, caracterizadas por tumefação celular com citoplasma amplo e eosinofílico, núcleo picnótico, ora excêntrico. Essas alterações astrocitárias foram evidenciadas pela técnica de imuno-histoquímica (IHQ), com imunomarcação fortemente positiva para os anticorpos anti-proteína S100 (anti-S100) e anti-proteína glial fibrilar ácida (anti-GFAP). O trabalho visa enfatizar em lesões encefálicas relacionadas à hepatotoxicidade aguda em bovinos.
    Pesquisa Veterinária Brasileira 01/2013; 33(1):47-51. · 0.54 Impact Factor
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    ABSTRACT: Os arquivos de biópsia do período de 2000 a 2010 do SPV-UFRGS foram revisados e levantados os casos de neoplasias mesenquimais de genitália (útero, cérvix, vagina e vulva) de cadelas. Realizou-se estudo retrospectivo de 43 casos, incluindo caracterização histológica, coloração tricrômico de Masson (TM) e imuno-histoquímica (IHQ). As principais raças acometidas foram Cocker, Poodle e Pastor Alemão, porém o maior número de casos foi observado em cães sem raça definida (SRD). A idade média dessas cadelas foi de 10,6 anos (variação de 3 a 10 anos). Quanto à localização dos tumores, 44,2% estavam na vagina, 27,9% no útero, 23,3% na vulva e 4,6% na cérvix. Histologicamente, 46,5% eram leiomioma, 41,9% fibroleiomioma, 7,0% fibroma e 4,6% leiomiossarcoma. Na coloração de TM, os fibromas apresentaram todas as células neoplásicas coradas em azul (colágeno), os leiomiomas e os leiomiossarcomas apresentaram menos de 50% de colágeno em meio às células neoplásicas musculares e os fibroleiomiomas mais de 50%. Na IHQ, os fibromas apresentaram marcação positiva para vimentina e negativa para desmina. Todos os leiomiomas eram positivos para vimentina (em mais de 50% células) e para desmina (75% dos casos em mais de 50% das células e 25% em menos de 50%). Em um dos casos de leiomiossarcoma houve imunomarcação para vimentina e ausente para desmina e, no outro, ocorreu o oposto. Os fibroleiomiomas apresentaram marcação para vimentina em 94,4% (em mais de 50% células) e para desmina em 77,8% (64,3% em menos de 50% das células e 35,7% em mais de 50%).
    Pesquisa Veterinária Brasileira 12/2012; 32(12):1313-1318. · 0.54 Impact Factor
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    ABSTRACT: ença altamente contagiosa, de curso rápido e pronta re-cuperação, causada pelo vírus influenza tipo A (SIV). Os principais sinais clínicos são tosse, febre, anorexia e baixo desenvolvimento. A doença está presente em outros países e, geralmente, está associada com outros agentes infeccio-sos. No Brasil, a primeira descrição ocorreu em 2011 e foi associada ao vírus H1N1 pandêmico (pH1N1). O principal objetivo deste estudo foi caracterizar as alterações histoló-gicas em casos de doença respiratória suína sugestiva de ABSTRACT.-Watanabe T.T.N., Almeida L.L., Wouters F., Wouters A.T.B., Zlotowski P. & Drie-meier D. 2012. Histopathological and immunohistochemical findings of swine with spontaneous influenza A infection in Brazil, 2009-2010. Pesquisa Veterinária Brasileira 32(11):1148-1154. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Bra-zil. E-mail: davetpat@ufrgs.br Swine influenza (SI) is caused by the type A swine influenza virus (SIV). It is a highly contagious disease with a rapid course and recovery. The major clinical signs and symptoms are cough, fever, anorexia and poor performance. The disease has been associated with other co-infections in many countries, but not in Brazil, where, however, the first outbreak has been reported in 2011. The main aim of this study was to characterize the histological features in association with the immunohistochemical (IHC) results for influenza A (IA), porcine circovi-rus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) in lung samples from 60 pigs submitted to Setor de Patologia Veterinária at the Universidade Fede-ral do Rio Grande do Sul (SPV-UFRGS), Brazil, during 2009-2010. All of these lung samples had changes characterized by interstitial pneumonia with necrotizing bronchiolitis, never observed previously in the evaluation of swine lungs in our laboratory routine. Pigs in this study had showed clinical signs of a respiratory infection. Swine samples originated from Rio Grande do Sul 31 (52%), Santa Catarina 14 (23%), Paraná 11 (18%), and Mato Grosso do Sul 4 (7%). Positive anti-IA IHC labelling was observed in 45% of the cases, which were associa-ted with necrotizing bronchiolitis, atelectasis, purulent bronchopneumonia and hyperemia. Moreover, type II pneumocyte hyperplasia, alveolar and bronchiolar polyp-like structures, bronchus-associated lymphoid tissue (BALT) hyperplasia and pleuritis were the significant features in negative anti-IA IHC, which were also associated with chronic lesions. There were only two cases with positive anti-PCV2 IHC and none to PRRSV. Therefore, SIV was the pre-dominant infectious agent in the lung samples studied. The viral antigen is often absent due to the rapid progress of SI, which may explain the negative IHC results for IA (55%); there-fore, IHC should be performed at the beginning of the disease. This study has shown how important a careful histological evaluation is for the diagnosis. Since 2009, a new histological feature of swine pneumonia in animals with respiratory clinical signs has been observed in samples from pigs with clinical respiratory disease submitted to SPV-UFRGS. In addition, the results proved the importance of histological evaluation for swine herd health management.
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    ABSTRACT: There is a current need for new therapeutic options for acetaminophen (APAP)-induced hepatotoxicity. Herein, we assessed the effects of prophylactic and therapeutic treatment with the angiotensin-converting enzyme (ACE) inhibitor, enalapril, on APAP-caused hepatotoxicity. Male and female C57BL/6 J mice were used, and hepatotoxicity was induced by a single application of APAP (400 mg/kg, i.p.). Macroscopic and histological liver alterations, serum alanine transaminase (ALT) and aspartate transaminase (AST) activity, liver catalase activity (CAT), reduced glutathione concentrations (GSH), hepatic measurement of neutrophil migration (myeloperoxidase, MPO activity), and caspase-3 liver expression were evaluated. The prophylactic and the therapeutic treatments with enalapril were able to markedly reduce the macroscopic and histological liver alterations as well as the caspase-3 immunopositivity. Both schedules of treatment were also effective in reducing GSH concentrations as well as neutrophil migration. Conversely, only the pre-treatment (but not the post-administration) with enalapril significantly reversed APAP-induced CAT decrease. Furthermore, the pre- or the post-treatment with enalapril largely reduced ALT and AST serum activity in APAP-intoxicated mice. The hepatoprotective effects of enalapril were comparable to those obtained with the clinically used compound N-acetylcysteine (NAC) when given in a therapeutic regimen. Data obtained with the prophylactic protocol of treatment might indicate that individuals under treatment with ACE inhibitors are less susceptible to the toxic effects of APAP. Additionally, the therapeutic approach allows us to suggest that enalapril might represent an innovative tool for treating APAP intoxication.
    Archiv für Experimentelle Pathologie und Pharmakologie 07/2012; 385(9):933-43. · 2.15 Impact Factor
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    ABSTRACT: A retrospective study of 24 cases of papillomas in dogs was performed from January 2001 to March 2011. Additionally, immunohistochemistry (IHC) was used to characterize and evaluate the samples. We found that disease was observed more in mixed breed dogs, ages ranging from 6 months to 10 years (mean 3.1 years), and there was no gender predilection. The main lesion sites were the skin (75%), lips (16.7%), and eyelids (8.3%). Upon histological evaluation, we observed papillary exophytic proliferation of squamous epithelium and papillary endophytic proliferation (inverted) in 87.5% and 12.5% of cases, respectively. The tumors were characterized by spinous layer hyperplasia (87.5%) with koilocytes (70.8%) and intranuclear pale basophilic inclusions bodies (8.3%), prominent granular layer with large amounts of keratohyalin granules (95.8%), and hyperkeratosis in the stratum corneum (100%). Positive immunostaining for Papillomavirus was found in 83.3% of cases, which were distributed between the granular layer and the stratum corneum. These findings indicate the following: that papillomas in dogs are caused by Papillomavirus, the viral cytopathic effect induces epithelial lesions, viral particles are found inside the cell nuclei, and inclusions bodies are rare.
    Pesquisa Veterinária Brasileira 07/2012; 32(7):653. · 0.54 Impact Factor
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    ABSTRACT: A total of 70 pigs were affected and died due to salt poisoning in two farms in southern Brazil. The only source of drinking water available to the pigs was whey from cheese salting process. One day after receiving the whey, animals started getting sick and showed excessive salivation, opisthotonus, paddling, and cyclic tremors. At necropsy of three pigs, two of them presented flattening of gyri and the other one presented cortical telencephalon edema. Microscopically, severe diffuse neuronal necrosis in telencephalic laminar cortex, Alzheimer type II astrocytes (AIIA), eosinophilic and lymphocytic perivascular cuffing apart of perivascular edema were observed. Weak or absent anti-glial fibrillary acidic protein (GFAP) immunolabeling was associated with positive immunostaining for S-100 in AIIA cytoplasm. Concentration of sodium in serum and liquor samples from dead pigs resulted 140 and 156mmol/L, respectively.
    Pesquisa Veterinária Brasileira 04/2012; 32(4):329-332. · 0.54 Impact Factor
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    ABSTRACT: Twenty-three dogs with infectious canine hepatitis (ICH) were analyzed, based on general data, clinicopathological findings, and immunohistochemistry. The main gross lesions were hepatic paleness (17/23), hepatomegaly (10/23), and hemorrhage (21/23). Gallbladder edema (11/23), fibrinous material on the hepatic capsular surface (8/23), and jaundice (6/23) were also found. Severe hepatic necrosis was observed in association with amphophilic or basophilic intranuclear inclusions inside hepatocytes and endothelial cells (22/23). Splenic and nodal lymphoid necrosis and hemorrhage associated with small number of intranuclear inclusions inside reticuloendothelial cells were identified. Immunohistochemistry (IHC) was positive for adenovirus type 1 in all animals, with the best staining pattern occurring in the liver (23/23), brain (22/22), cerebellum (16/20), and kidney (16/21). Greater amount of viral antigen was identified in hepatocytes and vascular endothelial cells from the brain and glomerular tuft.
    Pesquisa Veterinária Brasileira 02/2011; 31(2):158-164. · 0.54 Impact Factor
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    ABSTRACT: Lightning strike may cause sporadic deaths of animals that have been exposed to high-voltage electric currents during thunderstorms. Four healthy adult cattle from two herds were found dead next to eucalypt trees immediately after storms in Rio Grande do Sul, Brazil. There were recent burns and loose barks on the trunks of the trees, apart numerous branches and leaves scattered on the ground under the canopy of the trees. No gross or microscopic lesions were observed. In addition, there was no evidence of any toxic or infectious disease. These findings suggest that lightning strike caused the death of those animals.
    Pesquisa Veterinária Brasileira 03/2010; 30(3):243-245. · 0.54 Impact Factor