Chao Chen

Nanjing Agricultural University, Nan-ching, Jiangsu Sheng, China

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Publications (3)2.02 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Six1 protein belongs to the Six homeoproteins family, exposing typical domain structure. Although the functions of Six1 have been drawn much attention, the roles of its individual domains are not completely elucidated. Here, we first detected the expression patterns of myogenin, MyoD, Myf5, and Six1 genes using real-time PCR in differentiating C2C12 cells cultured in differentiation medium for 2 or 6 days. The results showed that Six1 gene had the similar expression pattern with myogenin, MyoD, and Myf5 genes, which suggests that it may affect the myogenic differentiation. In order to evaluate the role of distinct domains of Six1 protein in subcellular localization, we constructed a series of truncated vectors tagged with green fluorescent proteins expressing various regions of porcine Six1 protein for subcellular localization analysis. Fluorescence confocal microscopy analysis showed that the different regions of Six1 protein displayed discrete distributions throughout the nucleus and the cytoplasm. The full-length CDS was exclusively localized in the nucleus and the individual HD domain was preferentially distributed to the nucleus both in C2C12 cells and in PK cells. However, the SD domain was diffusely distributed to the cytoplasm and the nucleus, and the localization of SD domain was biased to cytoplasm in C2C12 cells. Taken together, we conclude that the HD domain is important for the nuclear localization of porcine Six1 protein.
    Molecular Biology Reports 06/2012; 39(12). DOI:10.1007/s11033-012-1868-5 · 2.02 Impact Factor
  • Chao Chen · Wang-Jun Wu · Yuan-Zhu Xiong ·

    Hereditas (Beijing) 12/2011; 33(12):1347-1352. DOI:10.3724/SP.J.1005.2011.01347
  • Chao Chen · Wang-Jun Wu · Yuan-Zhu Xiong ·
    [Show abstract] [Hide abstract]
    ABSTRACT: In order to understand the function of gene ATF4 and identify new DNA markers involved in pig production traits, the cDNA fragment of porcine ATF4 was cloned and sequenced. Sequence comparison revealed an A159G substitution downstream of the initiation codon (ATG). We then carried out PCR-AluⅠ-RFLP analysis in Large white, Landrace, Tongcheng and Meishan pigs, followed by association analysis in F2 "Large white ×Meishan" resource family. In all the individuals tested, Large White and Landrace pigs possessed the AA genotype, while Meishan and Tongcheng pigs pos-sessed the GG genotype. Association analysis in F2 resource family showed that this site was highly associated with buttock fat thickness (BFT) (Pamp;0.01) and had significant effect on thorax-waist fat thickness (TFT), average backfat thickness (ABT), loin eye height (LEH), and loin eye area (LEA)(Pamp;0.05). Real-time PCR was used to analyze the expression patterns of porcine ATF4 gene in longissimus dorsi at different development stages of Large White and Meishan pigs. The results showed that the gene expression levels of ATF4 were low 65 days after conception and 3 days after birth, but no signifi-cant differences were observed in both breeds. Meanwhile, the expression levels of porcine ATF4 gene were up-regulated 60 days and 120 days after birth in both breeds and the expression level in Meishan pigs was obviously higher than that in Large White pigs. These data could lay the foundation for further study on the molecular mechanism of porcine ATF4 gene in lipid metabolism.
    Hereditas (Beijing) 12/2011; 33(12):1347-52.

Publication Stats

5 Citations
2.02 Total Impact Points


  • 2012
    • Nanjing Agricultural University
      • College of Animal Science and Technology
      Nan-ching, Jiangsu Sheng, China
  • 2011
    • Huazhong Agricultural University
      • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education
      Wu-han-shih, Hubei, China