Faiz Muhammad

Ocean University of China, Tsingtao, Shandong Sheng, China

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Publications (13)3.95 Total impact

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    Faiz Muhammad, Muhammad Shafi
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    ABSTRACT: We conducted the histological observations on development of sperms in white leg shrimp L. vannamei, a prime aquaculture species. The testes have three distinctive stages of sperms, spermatogonium, spermatocytes and spermatids, in addition the nurse cells. The spermatozoa were observed in distal vas deferens (DVD) and in ejaculatory duct. The spike of the spermatozoa is prominent. We concluded that development of the sperms is a continuous process all along the reproductive tract.
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    ABSTRACT: In present investigation the proliferating cell nuclear antigene (PCNA) of white leg shrimp (Litopenaeus vannamei) (LvPCNA) has been cloned by rapid amplification of cDNA (RACE) and anchored PCR method. The full length LvPCNA had 1131 bp containing 786 bp of open reading frame (ORF), encoding 261 amino acids with an estimating molecular weight of 28.8 kDa. Tissue distribution of the LvPCNA after real time analysis revealed that expression is high in gill and muscle. The expression level after bacterial challenge of LvPCNA in hepatopancreas suggested the LvPCNA might be involved in defense mechanism of shrimp. The present investigation is a contribution to the existing knowledge of immune responses of shrimp to bacterial challenge.
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    ABSTRACT: Sex related FTZ-F1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, FTZ-F1 gene (FTZ-F1) was isolated from the testis of black rockfish (Sebastes schlegeli) by homology cloning. The full-length cDNA of S. schlegeli FTZ-F1 (ssFTZ-F1) contained a 232bp 5′ UTR, a 1449bp ORF encoding FTZ-F1 (482 amino acid residules in length) with an estimated molecular weight of 5.4kD and a 105bp 3′ UTR. Sequence, tissue distribution and phylogenic analysis showed that ssFTZ-F1 belonged to FTZ group, holding highly conserved regions including I, II and III FTZ-F1 boxes and an AF-2 hexamer. Relatively high expression was observed at different larva stages. In juveniles (105 days old), the transcript of ssFTZ-F1 can be detected in all tissues and the abuncance of the gene transcript in testis, ovary, spleen and brain was higher than that in other tissues. In mature fish, the abundance of gene transcript was higher in testis, ovary, spleen and brain than that in liver (trace amount), and the gene was not transcribed in other tissues. The highest abundance of gene transcript was always observed in gonads of both juvenile and mature fish. In addition, the abundance of gene transcript in male tissues were higher than that in female tissue counterparts (P<0.05).
    Journal of Ocean University of China 03/2013; 12(1).
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    ABSTRACT: The Litopenaeus vannamei is a prime aquaculture species and has a worth market value all over the world. In this study we investigated the genesis and morphology of hematopoietic tissue (HPT) and types of hemocyte based on its morphology and cytochemical feature using histological and histochemical methods. The results revealed that HPT in L. vannamei is not visible histologically in mysis and early two post larvae (P 1 & P 2), and emerges in P 3 which locates in epigastric region. The HPT showed a continuous propagation of cells in successive post larval stages. Four types of cells can be identified in the HPT from adult specimen and mitotic activity is visible in the HPT. The hemocytes have been differentiated into five types and some of these types are co-related with the HPT cells. The cytochemical studies suggested that type I and II cells of hemocytes are PAS positive while scanty presence of prophenoloxidase was observed in type I cells similarly the type I cells are Sudan Black B positive and rest of the cells showed weak activity against lipid detective stain. This is the first effort towards genesis of HPT and its relation with circulating hemocytes in L. vannamei. ©2012 PVJ. All rights reserved To Cite This Article: Muhammad F, ZF Zhang, MY Shao, XL Shi and M Shafi, 2012. Genesis of hematopoietic tissue and its relation with hemocytes of Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda). Pak Vet J, xx(x): xxx.
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    ABSTRACT: FOXL2 is a member of the forkhead box family, a transcription factor essential for the early regulation of ovarian development that is expressed in a sexually dimorphic manner in vertebrates. However, data on this gene in invertebrates are rare. In this study, we cloned a full-length cDNA sequence of foxl2 from the scallop Chlamys farreri, an important commercial mollusk in China. The cDNA sequence of Cf-foxl2 (C. farreri foxl2) has 1,824 bp with an open reading frame of 1,107 bp encoding 369 amino acid residues containing the conserved domain forkhead box. Semiquantitative RT-PCR showed that Cf-foxl2 was expressed mainly in the ovary. Using quantitative real-time PCR, we found that the highest expression was in the ovary of proliferative stage animals, about 62-fold higher than that in the testis and about twofold higher than that in the ovary of growing and mature stages. In situ hybridization revealed that Cf-foxl2 mRNA was located in the cytoplasm of follicle cells and germ cells in the ovary and testis except in the spermatozoa. Our data imply that Cf-foxl2 is expressed in a sexually dimorphic pattern at the RNA level, which is conserved with vertebrates.
    Development Genes and Evolution 07/2012; 222(5):279-86. · 1.70 Impact Factor
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    ABSTRACT: Thyroid hormones triiodothyronine (T 3) and thyroxine (T 4) play a vital role for developmental and physiological functions in vertebrates. They are mediated via thyroid hormone receptors (TR). In the present paper we describe the cloning and characterization of two TR genes, thyroid hormone receptor alpha (TRα) and thyroid hormone receptor beta (TRβ) from ovary mRNA of a ovoviviparous fish Sebastes schlegelii. Results revealed that main domains and motifs of the two genes were highly conserved. Quantitative real time PCR showed that the mRNA expression levels of both TRα and TRβ  were high at 5 dps and slightly lower and constant during 10, 20 and 33 dps. In 105 days old juveniles and three years mature fish, the expression level was higher in gonads, brain, liver and kidney than in other tissues. The highest expression was observed in the gonads. The developmental and tissue expression patterns indicated their involvement in the growth and gonadal development. TRα exhibited higher expression than TRβ at all developmental stages and in all detected tissues except female liver, which might indicate the functional differences of these two genes.
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    ABSTRACT: The Litopenaeus vannamei is a prime aquaculture species and has a worth market value all over the world. In this study we investigated the genesis and morphology of hematopoietic tissue (HPT) and types of hemocyte based on its morphology and cytochemical feature using histological and histochemical methods. The results revealed that HPT in L. vannamei is not visible histologically in mysis and early two post larvae (P 1 & P 2), and emerges in P 3 which locates in epigastric region. The HPT showed a continuous propagation of cells in successive post larval stages. Four types of cells can be identified in the HPT from adult specimen and mitotic activity is visible in the HPT. The hemocytes have been differentiated into five types and some of these types are co-related with the HPT cells. The cytochemical studies suggested that type I and II cells of hemocytes are PAS positive while scanty presence of prophenoloxidase was observed in type I cells similarly the type I cells are Sudan Black B positive and rest of the cells showed weak activity against lipid detective stain. This is the first effort towards genesis of HPT and its relation with circulating hemocytes in L. vannamei. ©2012 PVJ. All rights reserved To Cite This Article: Muhammad F, ZF Zhang, MY Shao, XL Shi and M Shafi, 2012. Genesis of hematopoietic tissue and its relation with hemocytes of Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda). Pak Vet J, xx(x): xxx.
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    ABSTRACT: In present investigation the proliferating cell nuclear antigene (PCNA) of white leg shrimp (Litopenaeus vannamei) (LvPCNA) has been cloned by rapid amplification of cDNA (RACE) and anchored PCR method. The full length LvPCNA had 1131 bp containing 786 bp of open reading frame (ORF), encoding 261 amino acids with an estimating molecular weight of 28.8 kDa. Tissue distribution of the LvPCNA after real time analysis revealed that expression is high in gill and muscle. The expression level after bacterial challenge of LvPCNA in hepatopancreas suggested the LvPCNA might be involved in defense mechanism of shrimp. The present investigation is a contribution to the existing knowledge of immune responses of shrimp to bacterial challenge.
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    ABSTRACT: Early development of nervous system in Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda) Larval nervous system, genesis, differentiation 1. INTRODUCTION The nervous system of vertebrates and invertebrates varies in structural complexity. In annelids, molluscs and arthorpods the nervous system is more centralized into one or several ganglia in order to co-ordinate and store information received from the environment (Castro and Huber, 2007). The basic investigations on genesis of nervous system are necessary in order to reveal its functional establishment, the hormonal regulation and physiological activities. Furthermore, the morphological assessment of NS provides the valuable information for phylogenetic relationships (Purschke et al., 2000) because meager changes occur during its development. Until now number of studies had conducted on development of arthropod nervous system including investigations on decapod crustacean, based on immunocytochemical and molecular studies, the wealth of knowledge on neurogenesis of crustaceans is continuously growing. Some of the popular articles on the subject are (Elofsson, The morphology of decapods nervous system is well known and the shrimp nervous system is composed of dorsal brain and ventral nerve cord, the dorsal brain can further be described into proto-deuto-and tritocerebrum (Young, 1959). Besides studies on adult nervous system, scanty information is available on the structural morphology and development of the larval nervous system in penaeidae shrimps. The investigations on the larval nervous system of penaeidae species are only restricted to the studies of (Elofsson, 1969; Nakamura and Seki, 1990 and Zhang et al., 2007). The literature suggested that appearance of nervous system related structures in larval stages are not specific. The present study designed to investigate the morphology and development of early nervous system in L. vannamei, a prime aquaculture species, using classical histological methods. The study provides academic evidences on probing into the functional establishment of nerve cell.
    Sindh University Research Journal (Science Series). 01/2012; 44(1):29-34.
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    ABSTRACT: The Litopenaeus vannamei is a prime aquaculture species and has a worth market value all over the world. In this study we investigated the genesis and morphology of hematopoietic tissue (HPT) and types of hemocyte based on its morphology and cytochemical feature using histological and histochemical methods. The results revealed that HPT in L. vannamei is not visible histologically in mysis and early two post larvae (P 1 & P 2), and emerges in P 3 which locates in epigastric region. The HPT showed a continuous propagation of cells in successive post larval stages. Four types of cells can be identified in the HPT from adult specimen and mitotic activity is visible in the HPT. The hemocytes have been differentiated into five types and some of these types are co-related with the HPT cells. The cytochemical studies suggested that type I and II cells of hemocytes are PAS positive while scanty presence of prophenoloxidase was observed in type I cells similarly the type I cells are Sudan Black B positive and rest of the cells showed weak activity against lipid detective stain. This is the first effort towards genesis of HPT and its relation with circulating hemocytes in L. vannamei. ©2012 PVJ. All rights reserved To Cite This Article: Muhammad F, ZF Zhang, MY Shao, XL Shi and M Shafi, 2012. Genesis of hematopoietic tissue and its relation with hemocytes of Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda). Pak Vet J, xx(x): xxx.
    Pakistan Veterinary Journal 01/2012; 1:2074-7764. · 1.37 Impact Factor
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    ABSTRACT: Early development of nervous system in Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda) Larval nervous system, genesis, differentiation 1. INTRODUCTION The nervous system of vertebrates and invertebrates varies in structural complexity. In annelids, molluscs and arthorpods the nervous system is more centralized into one or several ganglia in order to co-ordinate and store information received from the environment (Castro and Huber, 2007). The basic investigations on genesis of nervous system are necessary in order to reveal its functional establishment, the hormonal regulation and physiological activities. Furthermore, the morphological assessment of NS provides the valuable information for phylogenetic relationships (Purschke et al., 2000) because meager changes occur during its development. Until now number of studies had conducted on development of arthropod nervous system including investigations on decapod crustacean, based on immunocytochemical and molecular studies, the wealth of knowledge on neurogenesis of crustaceans is continuously growing. Some of the popular articles on the subject are (Elofsson, The morphology of decapods nervous system is well known and the shrimp nervous system is composed of dorsal brain and ventral nerve cord, the dorsal brain can further be described into proto-deuto-and tritocerebrum (Young, 1959). Besides studies on adult nervous system, scanty information is available on the structural morphology and development of the larval nervous system in penaeidae shrimps. The investigations on the larval nervous system of penaeidae species are only restricted to the studies of (Elofsson, 1969; Nakamura and Seki, 1990 and Zhang et al., 2007). The literature suggested that appearance of nervous system related structures in larval stages are not specific. The present study designed to investigate the morphology and development of early nervous system in L. vannamei, a prime aquaculture species, using classical histological methods. The study provides academic evidences on probing into the functional establishment of nerve cell.
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    ABSTRACT: Our investigation is focused on ontogenesis of the digestive system in Litopenaeus vannamei. The results reveal that in non-feeding stages the yolk mass is the indigenous source of food and until the fifth nauplius (N5) stage retained in foregut cavity, absorption is gradual in nauplius (N) stages while disappearance occurs at N6 stage. The anterior midgut caecum is discrete at N3 and it reaches maximum size in Z3; thereafter in successive stages it reduces in size and becomes a single structure in post-larval stage (P3–P4), named the anterior diverticulum. The lateral midgut caecum first appears at N6 stage and lengthens along both sides of the midgut and then ramifies to form lobes as larval development. Finally, these lobes become a compact structure, the hepatopancreas. The midgut lumen is quite apparent in N stages and only becomes wider in successive stages; it is without a cuticle lining and there is no difference among all investigated stages. The foregut in N stages is a simple tube and pyloric and cardiac chamber occurs in the third mysis (M3) stage. In the cardiac chamber of post-larval stages, folds were noted to increase gradually. The gastric mill is absent in all investigated larval stages (P1–P10). The pyloric chamber is smaller than the cardiac chamber and possesses a gland filter, which first appears in M3 stage and forms a more complete shape in forthcoming stages. The hindgut gland first appears in P2 and in successive stages it shows more protuberance and complexity. The hindgut or rectum is a simple tube. Cells of the digestive system in all feeding stages show the presence of acid mucins and general protein, while the inner margins of the foregut and connective tissues of the gland filter are positive for collagen.
    Italian Journal of Zoology 01/2011; · 0.89 Impact Factor