ABSTRACT: In the present study the bark of Aphanamixis polystachya was investigated for its macroscopic, phytochemical and antimicrobial characteristics. The dried stem bark of A. polystachya was successive extracted with petroleum ether, chloroform, alcoholic and hydromethanolic solvents for 48 hours in soxhlet apparatus and solvents from extracts was evaporated under vacuum. All four extracts were subjected to phytochemical investigation and in vitro antimicrobial screening using different bacterial strains in nutrient agar media. For screening of antimicrobial activity, extracts of dried stem bark of A. polystachya was used at the different doses (10, 20, 50 and 100mg/ml) against the kanamycin, which was used as standard antimicrobial agent at the dose of 30mg/ml. The zone of inhibitions indicates that the extracts of dried stem bark of Aphanamixis polystachya showed significant antimicrobial activity as comparison to kanamycin. The Preliminary phytochemical investigation revealed the presence of various phytoconstituents in each extracts. It showed the presence of carbohydrates, glycosides and saponins.
International Journal of Pharmacy and Pharmaceutical Sciences 03/2012;