ABSTRACT: Basidial macromycetes are not only value food, but can use as source of biological active compounds as the genestein,betta-glucans, glioxal–oxidase et al. Early we investigated the influence of the non-thermal extremely high frequency electromagnetic waves in the interval of 45-53GHz on mycelial culture’s growth, β-glucosidase and peroxidase activity of wood-decaying mushroom. The aim of this investigation is obtained extracts from mushroom culture Pleurotus ostreatus (Jacq.:Fr.) Kumm. with increased fermentative activity and studies its anti-inflammatory property.
Anti-inflammatory activity of mushroom’s extract investigate on the model of ear acute inflammation stimulated by xylol on white underbred rat-male with weight 150 – 180g. Anti-inflammatory activity estimated by mass difference of inflammatory and control ears after 90 min of fresh extract administration from mushroom’s culture, treated by different frequency of mm-waves. As evidence our investigations intraperitoneal introduction of extracts in a dose 2, 5 ml/kg led to the decreasing of induced by xylol ear swelling compare with control up to 85%. By HPLC analyze revealed protein enriched by histamine, which is probable responsible for such immune replay of organism.
Obtained data evidence about high anti-inflammatory activity of extracts from culture of wood-decaying mushroom’s, due to increased fermentative systems, having antioxidant activity and be able to inhibited ferments, participated in synthesis and metabolism of prostaglandins
FEBS&EFIS Workshop “Inflammatory diseases and immune responce: basic aspects, novel approaches and experimental models”, Vienna, Austria; 09/2010