[Show abstract][Hide abstract] ABSTRACT: The diversity of 87 Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains, pre-viously identified from different autochthonous dairy products, was investigated by phenotypic and genotypic approaches. The increased resolution obtained using phe-notypic and genotypic characterization allowed the level of strain heterogeneity detection to be widened. Phenotypic diversity was evaluated by studying biochemical charac-teristics of technological interest, including antimicrobial and proteinase activities, resistance to nisin, aggregation ability, production of exopolysaccharides, acetoin and diacetyl, citrate utilization, and antibiotic susceptibility. Genotypic diversity was generally evaluated by PCR amplification of repetitive bacterial DNA element finger-printing using the (GTG) 5 primer [(GTG) 5 -PCR]. More-over, in cases where strains were not discriminated by (GTG) 5 -PCR combined with phenotypic analysis, pulsed-field gel electrophoresis (PFGE) analysis was performed. The results indicate that L. plantarum/paraplantarum and L. paracasei natural isolates from artisanal dairy products are a gold mine in terms of diversity of strains and could be potentially interesting to dairy companies for the formu-lation of functional starter cultures in the production of innovative foods.
European Food Research and Technology 04/2012; 234:627-638. · 1.39 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A total of 157 lactic acid bacteria (LAB) were isolated from three hand-made cheeses taken from different
households in the region of the Caucasus Mountains. The cheeses were manufactured from cow’s milk without the addition of a starter culture. The isolates of LAB were characterized by subjecting them to phenotypic and genotypic tests. The results of identification of LAB indicate that the examined cheeses contained 10 species, viz., Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus farciminis, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Enterococcus faecium and Enterococcus faecalis. The strains within the species L. plantarum, L. arizonensis, L. paraplantarum, L. farciminis, and L. pseudomesenteroides showed good proteolytic activity.
Archives of Biological Sciences 06/2009; 61(3-61):395-406. · 0.79 Impact Factor