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Publications (2)5.94 Total impact

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    ABSTRACT: Extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase (AmpC)-producing and plasmid-mediated quinolone-resistant (PMQR) strains of Escherichia coli were investigated in wintering rooks (Corvus frugilegus) from eight European countries. 1073 feces samples from rooks wintering in the Czech Republic, France, Germany, Italy, Poland, Serbia, Spain and Switzerland were examined. Resistant isolates obtained from selective cultivation were screened for ESBL, AmpC and PMQR genes by PCR and sequencing. Pulsed-field gel electrophoresis and multi-locus sequence typing were performed to reveal their clonal relatedness. In total, 152 (14%, nsamples=1073) cefotaxime-resistant E. coli isolates and 355 (33%, nsamples=1073) E. coli with reduced susceptibility to ciprofloxacin were found. Eighty-two (54%) of these cefotaxime-resistant E. coli isolates carried ESBL genes as follow: blaCTX-M-1 (n=39), blaCTX-M-15 (25), blaCTX-M-24 (4), blaTEM-52 (4), blaCTX-M-14 (2), blaCTX-M-55 (2), blaSHV-12 (2), blaCTX-M-8 (1), blaCTX-M-25 (1), blaCTX-M-28 (1), and one not specified. Forty-seven (31%) cefotaxime-resistant E. coli isolates encoded AmpC beta-lactamases blaCMY-2. Sixty-two (17%) of E. coli isolates with reduced susceptibility to ciprofloxacin were positive for the PMQR genes qnrS1 (n=54), qnrB19 (4), qnrS1+qnrB19 (2), qnrS2 (1) and aac(6' )-Ib-cr (1). Eleven isolates from the Czech Republic (8) and Serbia (3) were identified as CTX-M-15-producing E. coli clone B2-O25b-ST131. Ninety-one different sequence types (ST) among 191 ESBL, AmpC and PMQR E. coli isolates were determined, with ST58 (n=15), ST10 (14) and ST131 (12) predominating. Widespread occurrence of highly diverse ESBL-, AmpC- and PMQR-positive E. coli, including the clinically important multi-resistant ST69, ST95, ST117, ST131 and ST405 clones, was demonstrated in rooks wintering in various European countries. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
    Applied and Environmental Microbiology 11/2014; · 3.95 Impact Factor
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    ABSTRACT: This study concerned the occurrence of fecal bacteria with plasmid-mediated quinolone resistance (PMQR) genes in rooks (Corvus frugilegus, medium-sized corvid birds) wintering in continental Europe during winter 2010/2011. Samples of fresh rook feces were taken by cotton swabs at nine roosting places in eight European countries. Samples were transported to one laboratory and placed in buffered peptone water (BPW). The samples from BPW were enriched and subcultivated onto MacConkey agar (MCA) supplemented with ciprofloxacin (0.06 mg/L) to isolate fluoroquinolone-resistant bacteria. DNA was isolated from smears of bacterial colonies growing on MCA and tested by PCR for PMQR genes aac(6')-Ib, qepA, qnrA, qnrB, qnrC, qnrD, qnrS, and oqxAB. All the PCR products were further analyzed by sequencing. Ciprofloxacin-resistant bacteria were isolated from 37% (392 positive/1,073 examined) of samples. Frequencies of samples with ciprofloxacin-resistant isolates ranged significantly from 3% to 92% in different countries. The qnrS1 gene was found in 154 samples and qnrS2 in 2 samples. The gene aac(6')-Ib-cr was found in 16 samples. Thirteen samples were positive for qnrB genes in variants qnrB6 (one sample), qnrB18 (one), qnrB19 (one), qnrB29 (one), and qnrB49 (new variant) (one). Both the qnrD and oqxAB genes were detected in six samples. The genes qnrA, qnrC, and qepA were not found. Wintering omnivorous rooks in Europe were commonly colonized by bacteria supposedly Enterobacteriaceae with PMQR genes. Rooks may disseminate these epidemiologically important bacteria over long distances and pose a risk for environmental contamination.
    Microbial drug resistance (Larchmont, N.Y.) 06/2012; · 1.99 Impact Factor