Kjell U. Sandvig MD

Publications (2)4.88 Total impact

• Article: Regeneration of the rat corneal epithelium after injury

  Kjell U. Sandvig MD, Erling Haaskjold, Rolf Bjerknes
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  ABSTRACT: Abstract Central corneal epithelial defects with diameter 3.5 mm were made with n-heptanol in the right eyes of rats. Erosions of the same size were made by mechanical scraping in the left eyes of the same animals. All the erosions were covered by epithelium after one day. After one day the mitotic rate and the labelling index were higher in the n-heptanol treated corneas compared to the mechanically abraded ones. Both methods produced similar mitotic rates and labelling indexes after 3 and 12 days. The results from wound healing studies using n-heptanol or mechanical scraping are therefore not entirely comparable because of the different regenerative responses after one day. However, the n-heptanol method is easier to perform and to standardize, and is therefore preferable.
  Acta ophthalmologica 05/2009; 69(6):717 - 722. · 2.44 Impact Factor
• Article: The proliferative response during regeneration of a ringshaped defect in the corneal epithelium

  Kjell U. Sandvig MD, Erling Haaskjold
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  ABSTRACT: Abstract The aim of the present study was to investigate the regenerative ability of the central corneal epithelium in an in vivo situation, as previous studies have been contradictory regarding the proliferative potential of these cells. Paracentral ringshaped defects were made in rat corneal epithelium, using filter paper soaked in n-heptanol. Cell counts, labelling indices and mitotic rates were measured in sections from the corneal and the adjacent conjunctival epithelium 12 and 28 h after the injury. The cell counts indicated that also central corneal epithelial cells migrate in the early phase of wound healing. No proliferative response was seen in the central corneal epithelium after 12 h. At 28 h both the peripheral and central corneal cells showed an enhanced labelling index, while the mitotic rate only was increased in the periphery. Different cell cycle times and different levels of differentiation of the cell population across the cornea may explain the delayed proliferative response in the central cornea.
  Acta ophthalmologica 01/1993; 71(1):39 - 43. · 2.44 Impact Factor