ABSTRACT: According to the previously published CSFV sequences, 18 pairs of primers have been designed and synthesized, which cover
the entire genome of CSFV strain Shimen. Each cDNA fragment has been amplified by RT-PCR from the anticoagulant blood of strain
Shimen infected pig. The PCR products have been cloned respectively and sequenced. Results show that the cDNA library of strain
Shimen and its nucleotide sequence have been obtained. The genomic RNA of strain Shimen is 12 298 nucleotides in length, containing
a 5′ and a 3′ noncoding region 373 and 231 nt long respectively. The center of genome is a single large open reading frame
of 11 697 nt which encodes a polyprotein of 3 898 amino acids. The entire sequence of strain Shimen has also been compared
with that of other CSFV strains.
Chinese Science Bulletin 04/2012; 45(4):367-369. · 1.32 Impact Factor