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ABSTRACT: A marine bacterium strain, designated as SCSIO 03483T, was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-colored pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and temperatures range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively higher sequence similarity with Imtechella halotolerans K1T (92.7%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequence revealed that the novel isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine (PE), two unidentified amino lipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω6c/C16 : 1 ω7c). The DNA G+C content of strain SCSIO 03483T was 38.4 mol %. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483T represents a novel species of a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain is SCSIO 03483T (= KCTC 23994T = CGMCC 1.12145T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor