Ye Liu

Zhejiang University, Hang-hsien, Zhejiang Sheng, China

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Publications (6)26.99 Total impact

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    ABSTRACT: Histone H3 lysine 4 methylation (H3K4me) is generally associated with actively transcribed genes in a variety of eukaryotes. The function of H3K4me in phytopathogenic fungi remains unclear. Here, we report that FgSet1 is predominantly responsible for mono-, di-, and trimethylation of H3K4 in Fusarium graminearum. The FgSET1 deletion mutant (ΔFgSet1) was crippled in hyphal growth and virulence. H3K4me is required for the active transcription of genes involved in deoxynivalenol and aurofusarin biosyntheses. Unexpectedly, FgSet1 plays an important role in response of F. graminearum to cell wall damaging agents via negatively regulating phosphorylation of FgMgv1, a core kinase in the cell wall integrity pathway. In addition, ΔFgSet1 exhibited increased resistance to the transcription elongation inhibitor mycophenolic acid. Yeast two-hybrid assays showed that FgSet1 physically interacts with multiple proteins including FgBre2, FgSpp1, and FgSwd2. FgBre2 further interacts with FgSdc1. Western blotting analyses showed that FgBre2 and FgSdc1 are associated with H3K4me. Both proteins are also involved in regulating deoxynivalenol biosynthesis and in responses to mycophenolic acid and cell wall damaging agents. Taken together, these data indicate that H3K4me plays critical roles not only in regulation of fungal growth and secondary metabolism but also in multiple stress responses in F. graminearum. This article is protected by copyright. All rights reserved.
    Environmental Microbiology 07/2015; DOI:10.1111/1462-2920.12993 · 6.24 Impact Factor
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    ABSTRACT: The RNA interference (RNAi) plays a critical role in gene regulation in a variety of eukaryotic organisms. However, the role of RNAi remains largely unclear in plant pathogenic fungi. In this study, we explored the roles of core components of the RNAi pathway in Fusarium graminearum, the major causal agent of wheat head blight. Our results demonstrated that the hairpin RNA (hpRNA) can efficiently silence the expression level of target gene, and the argonaute protein FgAgo1 and dicer protein FgDicer2 are important in this silencing process. RNAi machinery was not involved in growth, abiotic stress and pathogenesis in F. graminearum under tested conditions. We firstly applied high-throughput sequencing technology to elucidate small RNA (17-40 nucleotides) (sRNA) transcriptome in F. graminearum, and found that a total of forty-nine micro-like-RNA (milRNA) candidates were identified in the wild-type and ∆FgDICER2, and twenty-four of them were FgDicer2-dependent. Fg-milRNA-4 negatively regulated expression of its target gene. Taken together, our results indicated that the hpRNA-induced gene silencing was a valuable genetic tool for exploring gene function in F. graminearum. FgAgo1 and FgDicer2 proteins played a critical role in the hpRNA mediated gene silencing process. In addition, FgDicer2 was involved in sRNA transcription and milRNA generation in this fungus.
    Scientific Reports 07/2015; 5:12500. DOI:10.1038/srep12500 · 5.58 Impact Factor
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    ABSTRACT: The mitogen-activated protein kinase (MAPK) signaling pathways have been characterized in Fusarium graminearum. Currently, the upstream sensors of these pathways are unknown. Biological functions of a transmembrane protein FgSho1 were investigated using a target gene deletion strategy. The relationship between FgSho1 and the MAPK cassette FgSte50-Ste11-Ste7 was analyzed in depth. The transmembrane protein FgSho1 is required for conidiation, full virulence, and deoxynivalenol (DON) biosynthesis in F. graminearum. Furthermore, FgSho1 and FgSln1 have an additive effect on virulence of F. graminearum. The yeast two-hybrid, coimmunoprecipitation, colocalization and affinity capture-mass spectrometry analyses strongly indicated that FgSho1 physically interacts with the MAPK module FgSte50-Ste11-Ste7. Similar to the FgSho1 mutant, the mutants of FgSte50, FgSte11, and FgSte7 were defective in conidiation, pathogenicity, and DON biosynthesis. In addition, FgSho1 plays a minor role in the response to osmotic stress but it is involved in the cell wall integrity pathway, which is independent of the module FgSte50-Ste11-Ste7 in F. graminearum. Collectively, results of this study strongly indicate that FgSho1 regulates fungal development and pathogenicity via the MAPK module FgSte50-Ste11-Ste7 in F. graminearum, which is different from what is known in the budding yeast Saccharomyces cerevisiae.
    New Phytologist 11/2014; 206(1). DOI:10.1111/nph.13158 · 7.67 Impact Factor
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    ABSTRACT: Carbendazim has been used in the control of Fusarium head blight (FHB) for more than 30 years in China. Thus, carbendazim-resistant (Car(R) ) populations of Gibberella zeae have developed in some areas. In this study, 9341 G. zeae isolates were collected from the ten main wheat-producing regions of China in the period from 2008 to 2012, and sensitivity to carbendazim was detected. A high frequency of Car(R) isolates was observed in Zhejiang and Jiangsu provinces. Car(R) isolates were recovered from Anhui and Henan provinces in 2009 and 2012, respectively, but were not detected in the other six regions. Available (F167Y, E198Q and F200Y) and newly developed (E198L and E198K) allele-specific PCR assays were used to genotype field Car(R) isolates. The β-tubulin variants harbouring point mutation F167Y or E198Q accounted for >95% in Car(R) populations. Quantitative allele-specific real-time PCR assays were developed to determine the frequencies of five different β-tubulin variants present in populations of perithecia sampled from rice stubble. Car(R) populations of G. zeae develop rapidly under the selection pressure of carbendazim. Real-time PCR assays detecting the resistance frequencies in populations of perithecia would provide useful information for FHB control and management of resistance. © 2013 Society of Chemical Industry.
    Pest Management Science 08/2014; 70(8). DOI:10.1002/ps.3680 · 2.74 Impact Factor
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    ABSTRACT: To assess the potential risk of resistance development in Aspergillus flavus to pyrimethanil, five highly pyrimethanil-resistant (PyrR) mutants (RF > 996.2) were obtained after UV-mutagenesis and tested for fitness parameters and aflatoxin B1 production. All five mutant strains had mycelial growth rate, sporulation and aflatoxin production similar to or even higher than the wild-type parent strain, which indicated that pyrimethanil possesses a high risk in the development of resistance in A. flavus. Comparing the sequences of four key enzymes cystathionine β-lyase (CBL), cystathionine γ-synthase (CGS), methionine sulfoxide reductase (MsrB), and sulfate permease (SP2) involved in the biosynthesis and metabolism of methionine and sulfate assimilation revealed that no amino acid difference was found between the mutant and wild-type parent strains, suggesting that the four enzymes might not be related to the anilinopyrimidines (APs) resistance in A. flavus.
    Crop Protection 03/2014; 60(June):5-8. DOI:10.1016/j.cropro.2014.02.004 · 1.49 Impact Factor
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    ABSTRACT: The velvet complex containing VeA, VelB and LaeA has been showed to play critical roles in the regulation of secondary metabolism and diverse cellular processes in Aspergillus spp. In this study, we identified FgVelB, a homolog of Aspergillus nidulans VelB, from Fusarium graminearum using the BLASTP program. Disruption of FgVELB gene led to several phenotypic defects, including suppression of aerial hyphae formation, reduced hyphal hydrophobicity and highly increased conidiation. The mutant showed increased resistance to osmotic stress and cell wall-damaging agents, which may be related to a high level of glycerol accumulation in the mutant. Additionally, the mutant exhibited increased sensitivity to the phenylpyrrole fungicide fludioxonil. Ultrastructural and histochemical analyses revealed that conidia of FgVELB deletion mutant contained numerous lipid droplets. Pathogenicity assays showed FgVELB deletion mutant was impaired in virulence on flowering wheat head, which is consistent with the observation that FgVelB is involved in the regulation of deoxynivalenol biosynthesis in F. graminearum. All of the defects were restored by genetic complementation of the mutant with wild-type FgVELB gene. Yeast two hybrid assays showed that FgVelB does not interact with FgVeA. Taken together, results of this study indicated that FgVelB plays a critical role in the regulation of various cellular processes in F. graminearum.
    Fungal Genetics and Biology 06/2012; 49(8):653-62. DOI:10.1016/j.fgb.2012.06.005 · 3.26 Impact Factor