[show abstract][hide abstract] ABSTRACT: Dissolved organic matter (DOM) is one of the most interesting and difficult problems in recent years due to its important
functions in the ecological and environmental system and the complexity of its chemical composition and structure. It is well
accepted that fluorescence characteristics and molecular weight distribution are two important parameters in the DOM characterization.
However, the relationship between them is still unknown. In this study, fluorescence and molecular weight distribution of
DOM in Lake Hongfeng, Lake Baihua and their rivers, and their relationship were investigated using the combination of fluorescence
spectroscopy and high-performance size exclusion chromatography (HPSEC) with on-line UV absorbance and fluorescence detectors.
The results show that there were two obvious humic-like fluorescence peaks (Peaks A and B) in DOM from lake water. But there
was another obvious protein-like fluorescence peak (Peak C) in DOM from river water. The humic-like fluorescence material
consisted of DOM fraction with smaller molecular weight, ranging from 1.0 to 3.0 kDa, while the protein-like fluorescence
material mainly consisted of DOM fraction with MW larger than 2.0 kDa. The calculation of MW using HPSEC was related to the
UV absorbance wavelength chosen.
Chinese Science Bulletin 12/2005; 51(1):89-96. · 1.32 Impact Factor