Zdravko Petrinec

University of Zagreb, Zagrabia, Grad Zagreb, Croatia

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Publications (30)15.01 Total impact

  • Veterinární medicína 07/2011; 56(7):343-350. · 0.68 Impact Factor
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    ABSTRACT: Localization and activity levels of the following digestive enzymes in the intestine of free-living large-scaled gurnard were determined: non-specific esterase, alkaline and acid phosphatase as well as aminopeptidase. Enzymatic activity of the four enzymes was confirmed in all intestine parts but with different distribution through the enterocytes and varying in diverse intensity according to intestine part. This research is part of a broader research project on the biology of economically important fish from the Adriatic Sea. Our study reveals that in the large-scaled gurnard, the middle and posterior intestinal segments play the major role in digestion and absorption of proteins, whereas all parts of the intestine participate in lipid absorption and intracellular digestion. The high protein and lipid content in the diet of the large-scaled gurnard is most likely responsible for high activities of esterase, alkaline phosphatase and aminopeptidase, as they are involved in digestion and absorption of proteins and lipids.
    Anantomia Histologia Embryologia 03/2011; 40(4):314-20. · 0.88 Impact Factor
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    ABSTRACT: Previously, Nosema infections in European honey bees have been attributed solely to N. apis (Ellis and Munn, 2005), but it now appears that N. ceranae is an emerging pathogen that has increased its distribution (Klee et al., 2007). Nosema ceranae has not been detected in Croatia to date, but we have suspected its presence in pure or mixed infections with N. apis, because of a high percentage of Nosema infections being found during summer, and, because N. ceranae has been diagnosed in some neighbouring countries. The aim of this research was to determine the presence of N. ceranae, its prevalence and distribution in all 21 districts of Croatia, using a multiplex PCR. The diversity of Nosema species was studied using 204 honey bee samples originating mainly from bee colonies with different pathological problems like depopulation, weakness or high colony mortality. Samples of dead bees were collected during the first spring inspection of colonies, in February and March 2009. Each common sample represented one location or one apiary. Samples of honey bees were analyzed according to the OIE Manual (Anon, 2008). After light microscopy, a total of 150 spore samples from different localities were selected and investigated by multiplex PCR. Extraction of genomic DNA and further molecular analysis was performed as follows: for each of selected suspensions of isolated Nosema spores, an aliquot of 50 μl was transferred to a fresh tube, boiled at 100°C for 30 mins and centrifuged at 14,000 g for 10 mins. Thirty μl of supernatant was removed and supplemented with 10x TE buffer to a final concentration of 10mM Tris and 5mM EDTA, pH8. This supernatant served as source of template DNA and was stored at -20°C, or, used immediately for multiplex PCR (Higes et al., 2006). Primers were selected, taking into account that primer sequences were specific to each of the two species, and that both amplicons could be simultaneously amplified (Anon, 2008) and separated using agarose gel electrophoresis for visualization of results. The molecular size of PCR products were determined by electrophoresis in a 2% agarose TAE (Tris-acetate-ethylene diamine tetra-acetic acid) gel in standard TAE buffer, stained with SYBR green, and visualized using UviTec gel documentation system.
    Journal of Apicultural Research and Bee World. 08/2010; 49(4):340-341.
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    ABSTRACT: Nosema disease of European honey bees afflicts bees worldwide. Nosema ceranae is a recently described microsporidian parasite of the honey bee (Apis mellifera) and its geographical distribution is not well known. The disease may have many negative effects on bee colonies and cause high losses for apiculture and consequently in agriculture. With this in mind, a total of 204 samples of dead bees from different localities in Croatia were selected and investigated for distribution, prevalence and diversity of N. ceranae infection, using light microscopic examination and multiplex PCR. Our results show that N. ceranae is the only nosema species found to infect honeybees in our geographically varied collection. The nucleotide sequences of amplicons from Nosema-infested honeybee samples were 100% identical with the N. ceranae sequence deposited in the GenBank database. N. ceranae infected bees were found in samples collected from each of 21 districts, and in all three cli-matic areas, i.e., mediterranean, mountain, and continental parts regions of Croatia. Nosemosis is a parasitic disease of adult hon-ey bees (Apis mellifera) caused by two species of microsporidia, Nosema apis (Zander, 1909) and Nosema ceranae (Fries et al., 1996). The disease occurs throughout the world, including Croatia, and causes significant honey production and eco-nomic losses, such as reduced yields of honey and other bee products (Anderson and Giacon, 1992), and poor pollination resulting in lower quality and reduced yields in agriculture (Goodwin et al., 1990). Honey bees afflicted with nosemosis start to forage earlier (Fries, 1995), while pathological changes of their mid – gut epithelial cells, as well as digestive and metabolic disorders (Hassanein, 1951), cause malnutrition (Muresan et al., 1975) resulting in de-creased populations in honey bee colonies (Malone et al., 1995), leading to premature colony deaths (Morse and Shimanuki, 1990). Affected honey bees tend to die away from the hive, and there are no obvious signs of disease, making it difficult to notice, hence it is often referred to as "the silent killer"(Hornitzky, 2005). Spores enter the digestive tract of bees via contaminated food or liquid honey during trophallaxis (Fries et al., 1996; Sulimanovic et al., 1995). Factors favouring the spread of the disease include the robbing of honey bee colonies and poor beekeeping practices, as well as sudden temperature fluctuations, poor foraging, and the disturbance, and frequent movement of honey bee colonies (Sulimanovic et al., 1995). Previously, nosema infections in Europe were at-tributed just to N. apis (Ellis and Munn, 2005), but it appears that N. ceranae is an emerging pathogen that has increased its distribution to include European honey bees (Klee et al., 2007) and may be displacing N. apis in this area. N. ceranae was first detected in the European honey bee in Taiwan and Spain (Higes et al., 2006; Huang et al., 2007) and Klee et al. (2007) speculated that N. ceranae may be a long established and wide spread infection in European honey bees. N. apis is a parasite of the bee digestive tract and is considered not to be highly virulent and with a clear sesonality such that infections are least prevalent during the summer months.In contrast, N. ceranae is highly pathogenic when experimen-tally inoculated into European honey bees (Higes et al., 2007; Cornman et al., 2009). However, there are usually no symptoms of diarrhea or visible adult
    Veterinární medicína 01/2010; 55(9):457-462. · 0.68 Impact Factor
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    ABSTRACT: Nosema disease affects adult honey bees and due to its mostly incon-spicuous signs and the need for eradication by exchange of frames with brood from a disinfected hive and often use of new wax, beekeepers de-vote insufficient attention or often neglect the disease. Also, there is a problem of controlling nosemosis, especially caused with N. ceranae be-cause of its asymptomatic duration and prohibition of using antibiotics in the treatment of apian diseases in the European Union, as well as in Croatian regulations. We have predicted great results for use of protein pollen patties with "Nozevit" herbal preparation, as a feed supplement for bee colonies, where it can have an effect on brood rearing (colony strength) and at the same time reduce the number of Nosema ceranae spores. The aim of this study was to assess the effectiveness of the "Noze-vit" phyto-pharmacological preparation in protein/pollen substitute pat-ties for treatment of nosema disease in comparison with patties without "Nozevit" and sugar solution in a similar control group.
    American Bee Journal 12/2009; 149(11):1053-1056. · 0.09 Impact Factor
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    ABSTRACT: For years scientists have suspected that the environment plays a role in neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Mycotoxin fumonisin B1 (FB1) is produced by several Fusarium species, mainly by Fusarium verticilioides, which is one of the most common fungi associated with corn worldwide. Fumonisins are known to cause equine leukoencephalomalacia, a disease associated with the consumption of corn-based feeds contaminated with FB1. Here we have reported chronic experimental toxicosis in one-year-old carp (Cyprinus carpio L.) receiving feed containing 100 mg kg-1 or 10 mg kg-1 of added FB1 for 42 days. We focused on fumonisin toxicity in the fish brain. After staining with hemalaun-eosin, histology of the fish brain revealed vacuolated, degenerate, or necrotic neural cells, scattered around damaged blood capillaries and in the periventricular area. These findings suggest that fumonisin, although it is a hydrophilic molecule, permeated the blood-brain barrier of young carp and had a toxic effect on neuronal cells.
    Archives of Industrial Hygiene and Toxicology 12/2009; 60(4):419-26. · 0.67 Impact Factor
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    ABSTRACT: Garfish, Belone belone (Linnaeus, 1761) is an elongate, slander fish inhabiting the Eastern Atlantic and Mediterranean Sea. These fish from the Belonidae (Actinopterygii) family have important commercial value for Croatian fisheries. Samples for research were collected from experimental hatching in the Novigrad Sea. Preparation techniques included fixation in buffered formalin, trypsin clearing and staining with alcian blue and alizarin red. As little is known of garfish osteology and bone morphology, the main goal of this study was to describe ossification process in garfish fry. At hatching, no skeletal structure is present. Newly-hatched larvae also had no osteological elements. Ossification started at 7 day post-hatching (DPH) [total length (TL) 18 mm] with head bones and vertebral neural arch. Head skeleton continued to develop mostly over the period from 7 to 10 DPH. At 21 DPH (TL 49 mm), ossification process seemed to be finished, but it was not possible to distinguish borders of all bones. The primary interest of our research was to understand the growth dynamics as well as transformation of supporting body elements from cartilage to bone. At the end, developmental characteristics and functional aspects of this formation in different fish species are discussed.
    Anantomia Histologia Embryologia 09/2009; 38(5):351-4. · 0.88 Impact Factor
  • American Bee Journal 05/2009; 149(5):485 - 490. · 0.09 Impact Factor
  • Arhiv Za Higijenu Rada I Toksikologiju - ARH HIG RADA TOKSIKOL. 01/2009; 60(4):419-426.
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    ABSTRACT: The European catfish, Silurus glanis L., has become an important aqua cultural fish in Croatia, and it is cultivated extensively in ponds in polyculture with carps. The development of the digestive tract in S. glanis was studied with the aim of improving intensive fish production. Research was carried out on S. glanis larval stadium from 1- to 19-day post-hatching (DPH). The main histological methods used were: haematoxylin and eosin staining, periodic acid Schiff staining (PAS), Alcian blue (AB) and toluidin blue staining (TB). A yolk sac was present during the first 5 days (1-5-DPH). During the initial 3-DPH period, there was no trace PAS and AB activity in the digestive tract. Differentiation of the digestive tract began at 3- to 5-DPH. The oesophagus was positive for AB at 5-DPH, PAS and TB after 7-DPH. Differentiation of enterocytes began at 5-DPG and the intestines were complete at 11-DPH. Development of liver and pancreas was also studied. The analysis of data obtained in this study suggests that after 5-DPH catfish larvae have morphologically completed digestive tracts.
    Anantomia Histologia Embryologia 05/2008; 37(2):141-6. · 0.88 Impact Factor
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    ABSTRACT: Nitric oxide (NO) has been implicated in the pathogenesis of migraine and treatment with its exogenous donor glyceryl trinitrate (GTN) represents widely accepted experimental »migraine model«. In this study, glyceryl trinitrate was administered intraperitoneally to carps, serum nitrite and nitrate levels were determined, permeability of blood-brain barrier was investigated, and histological changes of brain tissue were analyzed. Serum nitrite and nitrate levels displayed characteristic biphasic pattern with moderate initial increase and maximal terminal increase, suggesting the GTN-induced endogenous NO synthesis. Increased permeability of the blood-brain barrier in GTN-treated animals was determined based on Evans blue capillary leakage into the brain tissue. Histological analysis revealed changes consistent with vasodilatation and oedema. Our study strongly supports the importance of the NO role in the pathogenesis of migraine attacks and increase in blood-brain barrier permeability during the attack. The study has also provided evidence that this mechanism of action is conserved to the lower vertebrate.
    Collegium antropologicum 02/2008; · 0.61 Impact Factor
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    ABSTRACT: The presence of an Eimeria sp. was found in adult large-scaled gurnards Lepidotrigla cavillone (Lacepède, 1801) from the Novigrad Sea, in Croatia. The detection of Eimeria sp. in the pyloric caeca and intestines of the large-scaled gurnards is also the first report of the parasite in this fish species. The analysis of histopathological changes in the digestive tract led to the conclusion that the parasite could be a serious pathogen in heavily infected large-scaled gurnards.
    Acta Parasitologica 01/2008; 53(1):81-84. · 1.00 Impact Factor
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    ABSTRACT: The presence of metacercariae of Centrocestus formosanus was detected on the gills of goldfish (Carassius auratus L.) imported from Singapore and obtained from two pet shops. This is the first report of the parasite in Croatia. The histopathological changes observed lead to the conclusion that the infection could be one of the reasons for the death of goldfish.
    Helminthologia 11/2007; 44(4):214-216. · 0.78 Impact Factor
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    ABSTRACT: The histological structure, as well as content and distribution of glycoproteins (GPs) in the digestive tract mucosa, of the Atlantic bluefin tuna were studied. The digestive tract is short in accordance with their predatory nature and consists of the oesophagus, stomach and intestine. The short oesophagus has deep longitudinal folds and contains an abundance of secretory cells which synthesize a large quantity of neutral as well as sulphated and silylated GPs. The stomach is an elongated sac-like structure with simple tubular glands surrounded by connective tissue. The neck cells of gastric glands synthesize a mixture of neutral and acidic GPs. The intestine is short with many intestinal villi. Many goblet cells are situated between superficial epithelial cells. The intestinal goblet cells were shown to secrete both neutral and acidic GPs
    Veterinary Archives (veterinarski.arhiv@vef.hr); Vol.77 No.5. 01/2007;
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    Zdravko Petrinec
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    ABSTRACT: The histological structure, as well as content and distribution of glycoproteins (GPs) in the digestive tract mucosa, of the Atlantic bluefin tuna were studied. The digestive tract is short in accordance with their predatory nature and consists of the oesophagus, stomach and intestine. The short oesophagus has deep longitudinal folds and contains an abundance of secretory cells which synthesize a large quantity of neutral as well as sulphated and silylated GPs. The stomach is an elongated sac-like structure with simple tubular glands surrounded by connective tissue. The neck cells of gastric glands synthesize a mixture of neutral and acidic GPs. The intestine is short with many intestinal villi. Many goblet cells are situated between superficial epithelial cells. The intestinal goblet cells were shown to secrete both neutral and acidic GPs.
    01/2007;
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    ABSTRACT: The body length and diameter of muscle fibres from the right hypaxial tail myomeres were measured in 100 sea bass larvae (Dicentrarchus labrax, L.) at ages of 40 to 90 days, from a fish farm in Croatia. The larvae were kept at a temperature of 19-20 degrees C and fed with standard feed for their respective ages. Body lengths of larvae 40-90 days old varied from 8 to 42 mm, increasing significantly in 10-day intervals (p < 0.001), except between days 50 and 60 when there was no increase in the body length. The measured diameters of muscle fibres ranged from 10 to 80 microm, the mean value increasing except between days 50 and 60. The fibre diameter in larvae aged up to 60 days was 10-40 microm, while that in larvae older than 70 days was 51-80 microm. In 90-day-old larvae there were no fibres of 10-20 microm in diameter; however, the number of fibers of 61-80 microm diameter increased. A statistically significant concordance was determined between the body length and fibre diameter of larvae 70 to 90 days old. Between days 50 and 60, arrested growth of the larval body and corresponding decrease in fibre diameter was observed. From day 45 until days 59, larvae were fed with Artemia nauplii and microparticles of formulated diets. From the day 60, minced fish was added to formulated diets. This change in diet probably resulted in the observed growth arrest.
    Veterinary Research Communications 07/2006; 30(5):523-9. · 1.08 Impact Factor
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    ABSTRACT: Histochemical localization of non-specific esterase, alkaline and acid phosphatase in the intestine of free-living goldline (Sarpa salpa L. 1758) was investigated. Fish were caught in the vicinity of the town of Zadar (Adriatic Sea, Croatia), and samples of three parts of the intestine proper (anterior, middle and posterior) as well as the rectum were used for presentation of non-specific esterases, alkaline phosphatase and acid phosphatase. Non-specific esterase activity was found in the cytoplasm and brush border of enterocytes in all investigated intestinal segments and the rectum. The activity was stronger in the middle and posterior part of the intestine but weaker in the anterior segment of the intestine as well as in the rectum. Intestinal alkaline phosphatase was detected in the brush border and supranuclear cytoplasm of enterocytes of all investigated intestinal segments. Enzymatic activity gradually decreased in a posterior direction. Acid phosphatase activity was observed as a fine granular reaction product in the supranuclear region of enterocytes and was almost equal in all investigated intestinal segments as well as in the rectum. The possible role of enzymes in intracellular digestion and transport is discussed.
    Journal of Applied Ichthyology 02/2006; 22(1):43 - 48. · 0.90 Impact Factor
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    ABSTRACT: Removing fish from their natural environment, and placing them in aquariums, where large number is concentrated on small space, causes not only stress but increases the possibility of disease. In these unnatural conditions but often adequate for parasite reproduction, parasites can cause diseases leading to death. In our work we investigated parasites presence in goldfish (Carassius auratus L.) kept in aquarium, from three different pet shops. The study showed presence of: Trypanoplasma sp., Trichodina sp., Ichthyophthirius multifiliis, Myxoboulus sp., Dactylogyrus sp. and Gyrodactylus sp. Considering the number of parasites found in examined fish, it can be possible that parasites can cause mortality in goldfish. Therefore, special caution has to be on quarantine and healthcare while importing especially exotic aquarium fish that may be infected with exotic parasites. In case of disease, proper treatment in due time has to be conducted.
    Ribarstvo (ribarstvo@agr.hr); Vol.64 No.1. 01/2006;
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    ABSTRACT: A young Griffon vulture died with signs of convulsion, vomiting, tremor, seizures propulsions and lack of co-ordination. Toxicological analysis of the stomach content revealed the presence of methomyl (9.3mg/kg), methomyloxime (6.7mg/kg) and deltamethrin (6.1mg/kg). Concentrations of methomyl and its intermediate methomyloxime, combined with results of other analysis, indicate methomyl as the cause of death of this bird.
    European Journal of Wildlife Research 01/2005; 51(3):210-212. · 1.36 Impact Factor
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    ABSTRACT: Year-1 carp were fed ratios containing 100mg/kg and 10 mg/kg of added fumonisin B1 for 42 days. The experimental and control fish were examined clinically during the experiment and at the end all fish were necropsied and histological changes recorded. Blood vessels, liver, exocrine and endocrine pancreas, excretory and haematopoietic kidney, heart and brain were sensitive both to 100 and 10mg/kg of FB1 in the diet and the rodlet cell (RC) frequency was considerably increased in and around damaged tissues. Many damaged blood vessels contained stacks of RCs above the endothelium. Other changes subsequent to fumonisin exposure that have not been previously reported include scattered lesions in the exocrine and endocrine pancreas, and interrenal tissue, probably due to ischemia and/or increased endothelial permeability. Presented findings indicate the need for more intensive studies of fumonisin-induced toxicity in cultured fish.
    DTW. Deutsche tierärztliche Wochenschrift 10/2004; 111(9):358-63. · 0.41 Impact Factor

Publication Stats

57 Citations
15.01 Total Impact Points

Institutions

  • 1999–2011
    • University of Zagreb
      • • Department of Biology and Pathology of Bees and Fish
      • • Department of Anatomy, Histology and Embriology
      • • Faculty of Veterinary Medicine (VEF)
      Zagrabia, Grad Zagreb, Croatia