Ph.D. Kenji Yanoh M.D

Osaka City University, Ōsaka, Ōsaka, Japan

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Publications (3)4.46 Total impact

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    ABSTRACT: This study was undertaken to clarify the origin of the chromophilic substance that stained in light green [light green body (LGB)] was observed in the condensed cluster of stromal cells and in the background of endometrial glandular and stromal breakdown cases. The material consists of cytologic smears of endometrial glandular and stromal breakdown (EGBD) from 58 samples in which a histopathological diagnosis was subsequently obtained by endometrial curettage.The following parameters were examined. (1) The occurrence and number of a LGB. As for the “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LBG in background,” it was observed with all fields on one slide per case. When it was observed more than one in a preparation, the occurrence was determined and the number was also calculated. (2) Immunostaining of paraffin-embedded tissue sections and cytologic preparations.1The occurrence of “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LGB in background” was 100%, 44.8% and 91.4%, respectively. The number of “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LGB in background” was 21.2, 2.0 and 4.0, respectively.2When histological sections from paraffin-embedded tissue were tested by immunocytochemistry, “LGB” stained weakly or negative for fibrinogen, whereas it stained positively for CD31 and factor VIII. In addition, it stained positively for CD42b.3When cytologic preparation was tested by immunocytochemistry, “LGB” stained positively for CD31, factor VIII and CD42b. However, as for the fibrinogen, quantitative and qualitative evaluation of the immunocytochemical stain was not feasible due to extensive nonspecific reaction in the whole preparation.The “LGB” in EGBD cases proved to be a thrombus mostly made up by platelets, and it seemed that the recognition of the LGB showed to be a useful cytomorphological criterion for an always more accurate diagnosis of EGBD cases. Diagn. Cytopathol. 2012. © 2010 Wiley Periodicals, Inc.
    Diagnostic Cytopathology 02/2012; 40(3):204 - 209. · 1.49 Impact Factor
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    ABSTRACT: It is well known that “condensed cluster of stromal cells (CCSC)” and “metaplastic clumps with irregular protrusion (MCIP)” in endometrial glandular and stromal breakdown (EGBD) cases may simulate “clumps of cancer cells (CCC)” in endometrioid adenocarcinoma grade 1 (G1), leading to difficulty in cytological interpretation. The aim of this study was undertaken to clarify the cytological immunoreactivity of nuclear findings about CCSC and MCIP which may be recognized in EGBD cases by using p53 protein and cyclin A in liquid-based cytologic (LBC) preparations. The material consists of cytologic smears of 20 cases of EGBD and 20 cases of G1 for which histopathological diagnosis was obtained by endometrial curettage at the JA Suzuka General Hospital. The evaluation of immunoreactivity was performed by using the intensity of nuclear staining and the nuclear labeling index (N-LI). The intensity of nuclear staining was scored as negative (0), weak (1), moderate (2), or strong (3). The N-LI was scored as less than 10% (0), from 10 to 25% (1), from 26 to 50% (2), or greater than 50% (3). The final score was calculated of the addition of both partial scores.Results are as follows: As for the p53 protein immunoreactivity, CCC (2.4 ± 1.4) was a significantly higher value in comparison with CCSC (0) and MCIP (0.8 ± 0.4), respectively. As for the cyclin A immunoreactivity, CCC (2.8 ± 1.1) was a significantly higher value in comparison with CCSC (0) and MCIP (0.6 ± 0.5), respectively. CCSC and MCIP in EGBD are misunderstood as cellular atypia and structural atypia on occasion; but, as for results of the immunoreactivity scores of p53 protein and cyclin A in our study, it seemed that those biochemical characters proved that the biological activity level was low (or degenerative).The results of the current study demonstrated that the cytological immunoreactivity of nuclear findings by p53 and cyclin A appear to be more useful for the LBC assessment of endometrial lesions, especially for the discrimination of EGBD and G1.Diagn. Cytopathol. 2011;. © 2011 Wiley-Liss, Inc.
    Diagnostic Cytopathology 09/2011; · 1.49 Impact Factor
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    ABSTRACT: The aim of this study was undertaken to clarify the cytological characteristic of the “condensed clusters of stromal cells,” which may be recognized in endometrial glandular and stromal breakdown (EGBD) cases. The material consists of 60 cases of cytologic smears for which histopathological diagnosis was obtained by endometrial curettage; they comprised 30 cases of EGBD and 30 cases of endometrioid adenocarcinoma grade 1 (G1).The following parameters were examined for “condensed clusters of stromal cells” in EGBD and for “clumps of cancer cells” in G1, respectively: (1) the occurrence of “condensed clusters of stromal cells”; (2) the nuclear shape; (3) a longer/shorter axis ratio in cell nuclei; (4) the area of cell nuclei; (5) the presence of overlapping nuclei; (6) nuclear crowding; (7) immunostaining.(1) “Condensed clusters of stromal cells” were only observed in EGBD. (2) A reniform nuclear shape was observed in 100% EGBD (P < 0.0001) in comparison to G1 (3%). (3) The longer/shorter axis ratio in cell nuclei, G1 (1.37 ± 0.2) was significantly lower in comparison to EGBD (1.53 ± 0.12, P = 0.0005). (4) Nuclear area in G1 (51.6 ± 11.9, P < 0.0001) was significantly higher in comparison to EGBD (24.3 ± 3.9 μm2). (5) The score of overlapping nuclei in EGBD (2.5 ± 0.49) was significantly higher in comparison to G1 (1.8 ± 0.44, P < 0.0001). (6) The nuclear crowding score was the same both in EGBD (2) and G1 (2) and these findings were not statistically significant. (7) Both CD10 and Wilms' tumor protein 1 were positive in the “condensed clusters of stromal cells” in the EGBD. The anti-cytokeratin staining was positive in “clumps of cancer cells” in the G1.The evaluation of the immunocytochemical findings by combining the Wilms' tumor 1 protein, CD10, and the anti-cytokeratin with the considered cytomorphologic features (reniform nucleus) may be useful for a correct diagnosis of EGBD in endometrial cytology. Diagn. Cytopathol. 2009. © 2009 Wiley-Liss, Inc.
    Diagnostic Cytopathology 07/2009; 37(12):891 - 896. · 1.49 Impact Factor