H. Yu

Northeast Normal University, Hsin-ching, Jilin Sheng, China

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Publications (38)93.06 Total impact

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    ABSTRACT: Background:Myosin X (MYO10) was recently reported to promote tumour invasion by transporting integrins to filopodial tips in breast cancer. However, the role of MYO10 in tumours remains poorly defined. Here, we report that MYO10 is required in invadopodia to mediate invasive growth and extracellular matrix degradation, which depends on the binding of MYO10's pleckstrin homology domain to PtdIns(3,4,5)P3.Methods:The expression of MYO10 and its associations with clinicopathological and biological factors were examined in breast cancer cells and breast cancer specimens (n=120). Cell migration and invasion were investigated after the silencing of MYO10. The ability of cells to form invadopodia was studied using a fluorescein isothiocyanate-conjugated gelatin degradation assay. A mouse model was established to study tumour invasive growth and metastasis in vivo.Results:Elevated MYO10 levels were correlated with oestrogen receptor status, progesterone receptor status, poor differentiation, and lymph node metastasis. Silencing MYO10 reduced cell migration and invasion. Invadopodia were responsible for MYO10's role in promoting invasion. Furthermore, decreased invasive growth and lung metastasis were observed in the MYO10-silenced nude mouse model.Conclusions:Our findings suggest that elevated MYO10 expression increases the aggressiveness of breast cancer; this effect is dependent on the involvement of MYO10 in invadopodial formation.British Journal of Cancer advance online publication, 12 June 2014; doi:10.1038/bjc.2014.298 www.bjcancer.com.
    British Journal of Cancer 06/2014; 111(3). DOI:10.1038/bjc.2014.298 · 4.82 Impact Factor
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    ABSTRACT: The posterior lateral line (PLL) system in zebrafish has recently become a model for investigating tissue morphogenesis. PLL primordium periodically deposits neuromasts as it migrates along the horizontal myoseptum from head to tail of the embryonic fish, and this migration requires activity of various molecular mechanisms. Histone deacetylases (HDACs) have been implicated in numerous biological processes of development, by regulating gene transcription, but their roles in regulating PLL during embryonic development have up to now remained unexplored. In this study, we used HDAC inhibitors to investigate the role of HDACs in early development of the zebrafish PLL sensory system. We further investigated development of the PLL by cell-specific immunostaining and in situ hybridization. Our analysis showed that HDACs were involved in zebrafish PLL development as pharmacological inhibition of HDACs resulted in its defective formation. We observed that migration of PLL primordium was altered and accompanied by disrupted development of PLL neuromasts in HDAC inhibitor-treated embryos. In these, positions of PLL neuromasts were affected. In particular, the first PLL neuromast was displaced posteriorly in a treatment dose-dependent manner. Primordium cell proliferation was reduced upon HDAC inhibition. Finally, we showed that inhibition of HDAC function reduced numbers of hair cells in PLL neuromasts of HDAC inhibitor-treated embryos. Here, we have revealed a novel role for HDACs in orchestrating PLL morphogenesis. Our results suggest that HDAC activity is necessary for control of cell proliferation and migration of PLL primordium and hair cell differentiation during early stages of PLL development in zebrafish.
    Cell Proliferation 11/2013; DOI:10.1111/cpr.12081 · 3.28 Impact Factor
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    ABSTRACT: We present a high power 880 nm diode-pumped passively mode-locked Nd:YVO4 oscillator, followed by an 880 nm diode-pumped Nd:YVO4 amplifier. In the oscillator, a maximum power of 8.7 W was obtained with a repetition rate of 63 MHz and pulse duration of 32 ps, corresponding to an optical efficiency of 36%. The beam quality factors M2 were measured to be and , respectively. The amplifier generated up to 19.1 W output power with the pulse width and repetition rate remaining unaltered after amplification.
    Laser Physics 05/2013; 23(7):075302. DOI:10.1088/1054-660X/23/7/075302 · 1.03 Impact Factor
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    ABSTRACT: Sensorineural hearing loss (SNHL) is one of the most common sensory defects in humans. Hair cells are vulnerable to various ototoxic insults. Effective prevention of hair cell loss remains an unmet medical need. Apoptotic hair cell death, which involves active regulation of transcription, accounts for the majority of aminoglycoside-induced hair cells loss. As one of the important epigenetic covalent modifications, histone methylation is involved in the regulation of gene expression, development and reaction to injury. In particular, H3K9 dimethylation (H3K9me2) is critical for euchromatin gene silencing. In the present study, we examined the roles of two highly homologous histone methyltransfereases responsible for this modification, G9a/G9a-like protein (GLP), in the reaction to aminoglycoside-induced hair cell damage. We observed a rapid increase of H3K9me2 upon hair cell damage in organotypic cochlear cultures. Treatment with the G9a/GLP-specific inhibitors, BIX01294 or UNC0638, reduced the level of H3K9me2 and prevented hair cells from death. Local delivery of BIX01294 also prevented neomycin-induced in vivo auditory hair cell loss in the organ of Corti in a mouse damage model. It is unlikely that BIX01294 functions through blocking aminoglycoside absorption as it does not interfere with aminoglycoside uptaking by hair cells in the organotypic cochlear cultures. Our data revealed a novel role of histone methylation in otoprotection, which is of potential therapeutic value for SNHL management.
    Cell Death & Disease 02/2013; 4(2):e506. DOI:10.1038/cddis.2013.28 · 5.18 Impact Factor
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    ABSTRACT: BACKGROUND: Ichthyosis vulgaris (IV; OMIM 146700) is a very common inherited skin disorder. Loss-of-function mutations in filaggrin gene (FLG) have been identified as the cause of IV. In the previous study, we found that the percentage of FLG null mutations in atopic dermatitis (AD) associated IV was lower than that in isolated IV. We speculated that some clinical manifestation of IV in AD patients was not induced by FLG mutations. OBJECTIVES: In order to clarify this issue, we collected 21 IV pedigrees, 33 sporadic isolated IV patients and 116 AD associated IV patients to analyze FLG mutation frequency and filaggrin expression in isolated IV and AD associated IV. METHODS: A comprehensive sequencing of the FLG gene in all patients was performed using an overlapping PCR strategy. We also studied profilaggrin ⁄filaggrin protein expression in the skin by immunohistochemistry and measured the mRNA expression by real-time PCR in seven patients including one patient with IV harboring c.3321delA, two patients with AD associated IV harboring c.3321delA and c.6834del5 and four AD associated IV patients without FLG mutations. RESULTS: The mutation percentage of FLG gene is 74.1% and 43.1% in isolated IV patients and AD associated IV patients. Immunohistochemical staining revealed that profilaggrin⁄filaggrin peptides were remarkably reduced in the all patients' epidermis. All patients with either AD or IV showed less FLG mRNA expression compared to the normal control. CONCLUSIONS: These results indicated that factors other than FLG gene mutations can down-regulate profilaggrin ⁄filaggrin expression, leading to the ichthyosiform phenotype in the context of AD.
    British Journal of Dermatology 01/2013; 168(6). DOI:10.1111/bjd.12206 · 4.10 Impact Factor
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    ABSTRACT: The mBin1b is a beta-defensin gene identified in the mouse epididymis. In the current report, its expression pattern and antibacterial activities were characterized, and a transgenic (TG) mouse model was developed in which mBin1b was exclusively overexpressed by up to 50-fold over normal levels in the caput epididymis. The experimental animals are healthy with normal reproductive activity, but are more resistant to epididymal infection from Escherichia coli than normal animals. The expression of IL1α and IL1β in the epididymis was decreased in the TG mice, which suggests that mBin1b has a role in the regulation of inflammatory response in the epididymis.
    Genes and immunity 04/2012; 13(6):445-51. DOI:10.1038/gene.2012.13 · 3.79 Impact Factor
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    ABSTRACT: With a fiber coupled laser diode array as the pump source, Nd-doped Lu2SiO5 (Nd:LSO) crystal lasers at 4F3/2→4I11/2 and 4F3/2→4I13/2 transitions were demonstrated. The active Q-switched dual-wavelength lasers at about 1.08 μm, as well as continuous-wave (CW) and active Q-switched lasers at 1357 nm are reported for the first time, to the best of our knowledge. Considering the small emission cross-sections and long fluorescence lifetime, this material possesses large energy storage ability and excellent Q-switched properties. The special emission wavelength at 1357 nm will have promising applications to be used in many fields, such as THz generation, pumping of Cr3+:LiSAF, repumping of strontium optical clock, laser Doppler velocimeter and distributed fiber sensor.
    Applied Physics B 04/2012; 107(1). DOI:10.1007/s00340-011-4831-6 · 1.63 Impact Factor
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    ABSTRACT: The Wenchuan Earthquake critically damaged soil environment, but weakly changed the aboveground growing environment of the trees. Whether and how earthquake-induced landslides impact tree organ nutrients related with the survival and recovery of trees in next years have never been examined globally, but that is important to understand how difficulty and diversity the ecological restoration is in the areas. This study demonstrated that the landslides significantly changed tree organ nutrients, but the changes varied with different tree organs and species. In Cupressus funebris, Mn content increased in needles, Fe and S contents did in branches, no nutrient changes occurred in roots. For Cryptomeria fortunei, foliar P content increased, nutrient contents did not alter in branches, total organic carbon decreased in roots. The increased foliar nutrients in both species imply that the nutrient changes are associated with the improvement of photosynthetic processes and enhancement of biomass allocation to roots so as to reinforce the survival and recovery ability of both species growing in the disturbed belowground environment.
    Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference onBiomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on; 01/2012
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    Ekoloji 01/2012; 21(84):1-8. · 0.71 Impact Factor
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    Ecological Indicators 01/2012; 15(1):263-270. · 3.23 Impact Factor
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    ABSTRACT: Mycobacterium bovis bacillus Calmette-Guérin (BCG) priming and subunit vaccine boosting strategies are urgently needed to improve the protective efficacy of BCG in adult population. However, the schedule of subunit vaccine boosting is not well investigated, especially the optimal immune responses and vaccine immunization schedules are still not clear. We have constructed a novel subunit vaccine candidate consisting of fusion protein Ag85B-Mpt64 (190-198)-Mtb8.4 (AMM) in a complex adjuvant composed of dimo-thylidioctyl ammonium bromide (DDA) and BCG polysaccharide nucleic acid (BCG-PSN). In this study, we compared the effect of different boosting schedules of the subunit vaccine in the prime-boost strategies. C57BL/6 mice were primed with BCG first and then boosted with the AMM vaccine once at 10th week, twice at 8th, 10th week, or thrice at 6th, 8th, 10th week, respectively. The immune responses were evaluated at the 14th and 20th weeks, respectively. Twelve weeks after the last immunization, the mice were challenged with virulent Mycobacterium tuberculosis strain H37Rv, and the protective effect was evaluated. The results showed that BCG priming and the AMM vaccine boosting twice induced the strongest antigen-specific IFN-γ and IL-2 production, down-regulated CD4+ CD25+ FoxP3+ regulatory T cells (Tregs) and had the best protective effect among all groups, while boosting thrice induced the strongest IL-4 production and did not improve BCG-primed protection significantly. Boosting BCG with the AMM vaccine twice instead of once or thrice induced strong Th1-type immunity and down-regulated Tregs significantly, which correlated with the best protection against M. tuberculosis infection in mice.
    Scandinavian Journal of Immunology 11/2011; 75(3):293-300. DOI:10.1111/j.1365-3083.2011.02666.x · 1.88 Impact Factor
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    ABSTRACT: The introduction of new treatments for esophageal cancer including surgery, chemotherapy, radiotherapy, or a combination of these modalities has not only improved patient survival, but may also increase the risk of the second primary cancers. The available evidence is conflicting with most risk estimates based on sparse numbers. Here we estimated standardized incidence ratios (SIRs) of second cancer among 24,557 esophageal cancer survivors (at least 2 months) in the Surveillance, Epidemiology, and End Results (SEER) Program between 1973 and 2007, who had been followed up for median 6.5 years (range 2 months-29.3 years). Second cancer risk was statistically significantly elevated (SIR = 1.34, 95% confidence interval [CI]= 1.25-1.42) among the survivors compared with the general population; the SIRs for cancers of oral and pharynx, stomach, small intestine, larynx, lung and bronchus, thyroid and prostate cancer were 8.64 (95% CI = 7.36-10.07), 2.87 (95% CI = 2.10-3.82), 3.80 (95% CI = 1.82-7.00), 3.19 (95% CI = 2.12-4.61), 1.68 (95% CI = 1.46-1.93), 2.50 (95% CI = 1.25-4.47), and 0.77 (95% CI = 0.65-0.90), respectively. Radiotherapy raised cancer risk of larynx (SIR = 3.98, 95% CI = 2.43-6.14) and thyroid (SIR = 3.57, 95% CI = 1.54-7.03) among all esophageal cancer survivors. For patients who had 5-9 years of follow up after radiotherapy, the SIR for lung cancer was 3.46 (95% CI = 2.41-4.82). Patients with esophageal cancer are at increased risks of second cancers of oral and pharynx, larynx, lung, and thyroid, while at a decreased risk for prostate cancer. These findings indicate that radiotherapy for esophageal cancer patients may increase risk of developing second cancers of larynx, lung, and thyroid. Thus, randomized clinical trials to address the association of radiotherapy and the risk of secondary cancer are warranted.
    Diseases of the Esophagus 11/2011; 25(6):505-11. DOI:10.1111/j.1442-2050.2011.01273.x · 2.06 Impact Factor
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    ABSTRACT: Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease. There have been few detailed reports of the clinical evaluation of Chinese patients with AD. To give a profile of the clinical features of Chinese AD patients in a university hospital setting. A total of 1008 cases met Hanifin and Rajka diagnostic criteria of AD were recruited at Xinhua Hospital, Shanghai Jiaotong University School of Medicine, China. In our survey, 22.7% patients were mild, 66.6% were moderate and 10.7% were severe according to the SCORAD index. Both the frequency and severity of the male patients were slightly higher. The frequency of asthma among the AD patients was 16.7% and it was increased with the age (χ2 = 205.20, P = 0.000). The frequencies of objective minor signs were demonstrated with age-related changes. Besides, three localized variants including eyelid eczema (49.8%), scalp dermatitis (49.7%), infra-auricular and retroauricular fissuring (44.8%) were commonly observed, especially in the infantile phase (P < 0.01). It was showed significant differences in serum total immunoglobulin E (IgE) and eosinophil cationic protein (ECP) levels of different age groups. The positive rate of Phadiatop was raised after 3 years old and that of the common food allergens were decreased after 6 years old. More males than females had ongoing AD in our survey. Most AD debuted in the first year of the cases. High incidence of the three clinical signs: eyelid eczema, scalp dermatitis and infra-auricular and retroauricular fissuring among the patients suggests it can be a potential valuable diagnostic clue to AD.
    Journal of the European Academy of Dermatology and Venereology 10/2011; 25(10):1206-12. DOI:10.1111/j.1468-3083.2010.03953.x · 3.11 Impact Factor
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    ABSTRACT: A new kind of portable e-Tongue based on multi-channel LAPS array with PVC membrane has been designed for the rapid detection of environment situation, especially the seawater. It has the great advantages of depositing membranes which are offered by Chemistry Department, Saint-Petersburg State University on the sensors artificially with convenience and efficiency. To detect various heavy metal ions (Pb2+, Cd2+, Zn2+) simultaneously, respective Polyvinyl Chloride (PVC) membrane could be prepared on the surface of the silicon-based sensor in different channel.
    09/2011; DOI:10.1063/1.3626352
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    ABSTRACT: Polymer gels are used to stop unwanted fluid production from oil and gas wells. One of the most important types of gel is composed of polymer and phenol formaldehyde resin, which is synthesized by phenol and formaldehyde under alkaline conditions. The gelation time is in a wide range of several days to 10 days. Cross-linking occurs in a wide range of pH values and is sensitive to temperature. The phenol formaldehyde resin gel has good rheological behavior and thermal stability. Core flooding experiments show that the mentioned gel has good injectivity and plugging ability, especially selective plugging ability.
    Petroleum Science and Technology 04/2011; 29(12-12):1285-1297. DOI:10.1080/10916466.2010.525587 · 0.33 Impact Factor
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    ABSTRACT: In an earlier study we showed that C10ORF97 (chromosome-10, open reading frame-97) was expressed in almost all of the tissues and cell lines tested, and that it inhibited the growth of seven tumor cell lines, including two lung carcinoma cell lines (A549 and PG). Here, we show that C10ORF97 is downregulated in non-small-cell lung cancer (NSCLC) tissue compared with normal lung tissue. Overexpression of C10ORF97 significantly suppressed human lung carcinoma A549 cell growth (proliferation and anchorage-independent growth in soft agar) and motility (migration and adhesion). This tumor-suppressive function of C10ORF97 was also verified in vivo. We further found that C10ORF97 caused G(1) arrest of A549 cells and modulated the expression level of several cell-cycle regulators (such as CDK2, cyclin-E and p27). These effects of C10ORF97 were mediated by physical association between C10ORF97 and Jun-activating domain-binding protein-1 (JAB1), and blocking of JAB1-mediated translocation of p27 from the nucleus to the cytoplasm. Together, these results indicated that C10ORF97 functions as a novel tumor suppressor by modulating several key G(1)/S-regulatory proteins by interacting with JAB1. These findings led us to hypothesize that a single-nucleotide polymorphism (SNP) in the C10ORF97 gene that affects its expression might be associated with susceptibility to NSCLC. SNP216 C>T (rs2297882) in the C10ORF97 Kozak sequence was identified, and allele T of SNP216 suppressed C10ORF97 expression in vitro and in vivo. Furthermore, the TT genotype of SNP216 was associated with an increased risk of NSCLC (adjusted odds ratio=1.73 (95% confidence interval: 1.33-2.25), P=4.6 × 10(-5)). These data indicated that C10ORF97 is a tumor suppressor of NSCLC progression and C10ORF97-SNP216 may serve as a predictor of NSCLC.
    Oncogene 04/2011; 30(39):4107-17. DOI:10.1038/onc.2011.116 · 8.56 Impact Factor
  • Journal of Hepatology 03/2011; 54. DOI:10.1016/S0168-8278(11)60091-3 · 10.40 Impact Factor
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    ABSTRACT: Microgel solution composed of hydrophobically associating polymer and chromium acetate was prepared by gelling under shear provided by a peristaltic pump running at required speed. Experiments were conducted to determine microgel size, injectivity, plugging ability during polymer flooding, and ability of enhanced oil recovery. Results showed that the size of the microgel obtained was in the range of 0.1–170 μm by adjusting the rotational speed of the peristaltic pump. In polymer flooding, the microgel solution was intruded into the sand pack easily and could improve the plugging of following polymer flooding. Furthermore, the injection of microgel solution was useful to enhance oil recovery in the process of the following polymer flooding.
    Petroleum Science and Technology 02/2011; 29(7-7):715-727. DOI:10.1080/10916466.2010.531343 · 0.33 Impact Factor
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    ABSTRACT: Subunit vaccines have the potential advantage to boost Mycobacterium bovis Bacillus Calmette-Guérin (BCG)-primed immunity in adults. However, most candidates are antigens highly expressed in replicating bacilli but not in dormant or persisting bacilli, which exist during Mycobacterium tuberculosis infection. We constructed M. tuberculosis fusion protein Ag85B-Mpt64(190-198) -HspX (AMH) and Ag85B-Mpt64(190-198) -Mtb8.4 (AMM), which consist of Ag85B, the 190-198 peptide of Mpt64, HspX (Rv2031c) and Mtb8.4 (Rv1174c), respectively. AMH and/or AMM were mixed with adjuvants composed of dimethyl-dioctyldecyl ammonium bromide and BCG polysaccharide nucleic acid (DDA-BCG PSN) to construct subunit vaccines. Mice were immunized thrice with Ag85B, AMH and AMM vaccines and the immunogenicity of the fusion protein vaccines was determined. Then, mice were primed with BCG and boosted twice with Ag85B, AMH, AMM and AMM + AMH vaccines, respectively, followed by challenging with M. tuberculosis virulent strain H37Rv, and the immune responses and protective effects were measured. It was found that mice immunized with AMH vaccine generated high levels of antigen-specific cell-mediated responses. Compared with the group injected only with BCG, the mice boosted with AMM, AMH and AMM + AMH produced higher levels of Ag85B-specific IgG1 and IgG2a and IFN-γ-secreting T cells upon Ag85B and Mycobacterium tuberculosis purified protein derivative (PPD) stimulation. It is interesting that only mice boosted with AMM + AMH had significantly lower bacterial count in the lungs than those receiving BCG, whereas mice boosted with AMH or AMM did not. The results suggest that AMH consisting of HspX, the antigen highly expressed in dormant bacilli, could be combined with antigens from replicating bacilli to enhance BCG primed immunity so as to provide better protection against both growing and non-growing bacteria that occur during the infection process.
    Scandinavian Journal of Immunology 02/2011; 73(6):568-76. DOI:10.1111/j.1365-3083.2011.02531.x · 1.88 Impact Factor

Publication Stats

274 Citations
93.06 Total Impact Points


  • 2014
    • Northeast Normal University
      • The Institute of Genetics and Cytology
      Hsin-ching, Jilin Sheng, China
  • 2013
    • Chinese Academy of Sciences
      • Laboratory of All-Solid-State Laser Sources
      Peping, Beijing, China
    • Fudan University
      • Department of Otolaryngology
      Shanghai, Shanghai Shi, China
  • 2011–2013
    • Shanghai Jiao Tong University
      Shanghai, Shanghai Shi, China
    • Peking Union Medical College Hospital
      Peping, Beijing, China
    • Yale University
      New Haven, Connecticut, United States
    • Zhejiang University
      • Department of Biomedical Engineering
      Hang-hsien, Zhejiang Sheng, China
    • University of Petroleum (East China)
      Tsingtao, Shandong Sheng, China
    • Lanzhou University
      Kao-lan-hsien, Gansu Sheng, China
  • 2012
    • Shanghai Institute of Planned Parenthood Research
      Shanghai, Shanghai Shi, China
  • 2010–2012
    • Shandong University
      • State Key Laboratory for Crystal Materials
      Chi-nan-shih, Shandong Sheng, China
    • Sun Yat-Sen University
      • Department of Thoracic Surgery
      Shengcheng, Guangdong, China
  • 2008–2010
    • Tsinghua University
      • Department of Precision Instruments and Mechanical Engineering
      Peping, Beijing, China
  • 2008–2009
    • Northwest A & F University
      • College of Animal Science and Technology
      Yang-ling-chen, Shaanxi, China
    • Wuhan University
      • School and Hospital of Stomatology
      Wu-han-shih, Hubei, China