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Publications (2)4.18 Total impact

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    ABSTRACT: The antiviral activity of native and esterified whey proteins fractions (a-lactalbumin, b-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (TYLCV) on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using a-lactoferrin and a-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native b-lactoglobulin showed inhibition after 7 and 15 days whereas esterified b-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified a-lactalbumin whereas native a-lactalbu-min inhibited virus replication completely after 15 days. These results indicate that native or modified whey pro-teins fractions can be used for controlling the TYLCV-infected plants.
    Virology Journal 02/2010; · 2.09 Impact Factor
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    ABSTRACT: Abstract The antiviral activity of native and esterified whey proteins fractions (α-lactalbumin, β-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (TYLCV) on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using α-lactoferrin and α-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native β-lactoglobulin showed inhibition after 7 and 15 days whereas esterified β-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified α-lactalbumin whereas native α-lactalbumin inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants.
    Virology Journal 01/2010; · 2.09 Impact Factor