[Show abstract][Hide abstract] ABSTRACT: Many people who live in the low altitude areas are often suffered from hypoxia when they entered the high plateau. This problem may seriously influence the physical and mental state and work efficacy for the travelers and workers. Oxygen enrichment of a small space air at high altitude is considered as a simple way to provide lowlanders enriched oxygen for sleeping and resting, improving work efficiency, so we developed an oxygen concentration machine based on the technology of oxygen enrichment mem-brane. This paper tested 8 healthy male lowlanders (age 21.63±1.77 yr) who have never exposed to pla-teau performed an incremental exercise on cycle er-gometer at sea-level in order to be used as sea-level controls. Two days later, the same subjects were tak-en to Lhasa (3700 m) by air and exposed to the pla-teau, performed the same exercise as they did at sea-level. The next day, all subjects were asked to enter the experimental tent which was enriched with oxygen (higher than 24%) by the oxygen concentra-tion machine and sleep for 10 hours at night, then exposed to plateau and performed the same exercise twice at different time (2 hours and 10 hours after oxygen enrichment). During the tests, subjects must cycled continuously at 60 rpm beginning with a 3 min exercise intensity of 0 W followed by incremental in-creases of 25 W every 3 min until 150 W, pulse oxy-gen saturation (SpO 2) and heart rate (HR) were re-corded. After sleeping in an oxygen enrichment of tent air, 2 hours later, the subjects' load capacity had no difference compared with control group, but sig-nificant difference than before (higher SpO 2 and lower HR), which indicated that oxygen concentra-tion machine is effective in increasing the oxygen concentration of the air for the tent and sleeping in the oxygen enrichment tent for l0 h might be effective in improving exercise performance during high-alti-tude hypoxia. At the same time, 10 hours later, when work-load exceeded 125 W, the same effects were also found. The results indicated the effects of oxygen en-richment of tent air could last a certain period of time.
Journal of Biomedical Science and Engineering 01/2011; 04(02). DOI:10.4236/jbise.2011.42019
[Show abstract][Hide abstract] ABSTRACT: To study effects of oxygen-increased respirator on blood oxygen saturation (SaO2) and heart rate under altitude hypoxic environment.
Nine subjects were carried to the plateau of 3700 m by air for the first time, and then four trials were carried out two hours later. First, SaO2 and heart rate were examined during rest without oxygen-increased respirators, and then the examination was repeated by using the respirators. Second, the examinations were repeated during loaded exercise test without respirators. After rest for one hour, test of using the respirators during motion was carried out finally.
During rest, after using respirators, the level of SaO2 increased significantly while heart rate decreased notably (P<0.05) than those without respirators. During loaded-bicycle exercise, after using respirators, the level of SaO2 increased significantly (P<0.05) than that without respirators, while heart rate didn't show significant difference (P>0.05). But, the recoveries of heart rate after three minutes and fives minutes were better than those without respirators (P<0.05).
Oxygen-increased respirator can improve the capacity of workload under altitude hypoxic environment. It can promote the acclimation to high altitude.
Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering 09/2005; 18(4):297-9.
[Show abstract][Hide abstract] ABSTRACT: To prepare rabbit anti-Red antisera.
The bet, exo and gam genes of lambda phage were amplified by PCR from genomic DNA and cloned into the expression vector pDH2, respectively. Red proteins were induced to express at 42 degrees C. The expressed proteins were analyzed by PAGE and thin-layer scanning. The antisera were prepared by immunizing rabbits with the three Red proteins, respectively. The titers and specificities of the antisera were detected by Western blot.
Beta, Exo and Gam proteins accounted for about 40.3%, 49.2% and 73.4% of total bacterial protein, respectively. The titers of the antisera were about 1:2,000. Western blot analysis indicated that the three antisera all had good specificities to the corresponding proteins.
Specific anti-Red antisera are prepared successfully.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 06/2005; 21(3):305-8.