DMSc Diana Messadi DDS

Publications (2)5.5 Total impact

• Article: In vivo optical coherence tomography for the diagnosis of oral malignancy

  PhD Petra Wilder-Smith DDS, Woong-Gyu Jung MSc, Matthew Brenner MD, Kathryn Osann PhD, Hamza Beydoun BS, DMSc Diana Messadi DDS, Zhongping Chen PhD, Petra Wilder‐Smith, Woong‐Gyu Jung, Matthew Brenner, Kathryn Osann, Hamza Beydoun, Diana Messadi, Zhongping Chen
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  ABSTRACT: Background and Objective Oral cancer results in 10,000 U.S. deaths annually. Improved highly sensitive diagnostics allowing early detection of oral cancer would benefit patient survival and quality of life. Objective was to investigate in vivo non-invasive optical coherence tomography (OCT) techniques for imaging and diagnosing neoplasia-related epithelial, sub-epithelial changes throughout carcinogenesis.Study Design/Materials and Methods In the standard hamster cheek pouch model for oral carcinogenesis (n = 36), in vivo OCT was used to image epithelial and sub-epithelial change. OCT- and histopathology-based diagnoses on a scale of 0 (healthy) to 6 (squamous cell carcinoma, SCC) were performed at all stages throughout carcinogenesis by two blinded investigators.ResultsEpithelial, sub-epithelial structures were clearly discernible using OCT. OCT diagnosis agreed with the histopathological gold standard in 80% of readings.Conclusion In vivo OCT demonstrates excellent potential as a diagnostic tool in the oral cavity. Lasers Surg. Med. 35:269–275, 2004. © 2004 Wiley-Liss, Inc.
  Lasers in Surgery and Medicine 09/2004; 35(4):269 - 275. · 2.75 Impact Factor
• Article: In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

  PhD Petra Wilder-Smith DDS, Kathryn Osann PhD, Nevine Hanna BS, Naglaa El Abbadi BS, Matt Brenner MD, DMSc Diana Messadi DDS, Tatiana Krasieva PhD
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  ABSTRACT: Background and Objective Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality.Study Design/Materials and Methods In the hamster model (n = 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM- and histopathology-based diagnoses on a scale of 0 (healthy)–6 (squamous cell carcinoma [s.c.c.]) were scored by two pre-standardized investigators.ResultsCollagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses.Conclusions In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics. Lasers Surg. Med. 35:96–103, 2004. © 2004 Wiley-Liss, Inc.
  Lasers in Surgery and Medicine 07/2004; 35(2):96 - 103. · 2.75 Impact Factor