Rayza González

Publications (1)1.5 Total impact

• Article: Effect of Azotobacter chroococcum on in vitro pineapple plants’ growth during acclimatization

  Rayza González, Taletha Laudat, Mayda Arzola, Roberto Méndez, Pedro Marrero, Lázaro E. Pulido, Bernardo Dibut, José Carlos Lorenzo
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  ABSTRACT: Pineapple is one of the most important tropical fruits, but the availability of planting material is insufficient to agricultural demands. Therefore, several pineapple micropropagation protocols have been developed. However, acclimatization of in vitro plants continues to take a prolonged period. Biofertilizers have been found as safe alternatives to improve the agricultural performances of many crops. This study highlights some of the effects of the application of Azotobacter chroococcum (INIFAT5 strain) on in vitro pineapple plants during acclimatization. The bacteria were sprayed immediately after transplanting to the ex vitro environment; the plants were then sprayed every 4wk. A control group of plants was established. Subsequently, after 5mo, the evaluated variables included fresh and dry plant weight, plant height (cm), and root length (cm). The anatomy of middle-aged leaves and roots was also studied: transversal thickness and width of cuticle, epidermis, hypodermis, aquiferous parenchyma, and photosynthetic parenchyma. Thickness of root exoderm, external cortex, internal cortex, and stele were also evaluated. In general, the INIFAT5 strain improved the plant development. Results showed that the bacteria significantly provoked changes in the plant fresh weight, the thickness of the leaf abaxial and adaxial cuticles, and the root exoderm width. Contrastingly, A. chroococcum did not affect the thickness of the leaf photosynthetic parenchyma. Keywords Ananas comosus (L.) Merr.–Biofertilizer–Histology
  In Vitro Cellular & Developmental Biology - Plant 05/2012; 47(3):387-390. · 1.50 Impact Factor