T V Sirota

Russian Academy of Sciences, Moskva, Moscow, Russia

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Publications (38)31.06 Total impact

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    ABSTRACT: In this paper the oil from seeds of Amaranthus cruentus L. (AmO) was shown to be an efficient modulator of the physical chemical properties of artificial lipid and rat hepatocyte plasma membranes. AmO improved the membrane stability, their stress resistance and the adsorption of neurotensin to plasma membranes with the distinct biphasic interactions being observed even after adrenalin stress exposure. The analysis of pro-/antioxidant balance in rat blood revealed a mild prooxidant activity after AmO intake, which was accompanied by accumulation of oxidative destruction products in plasma membranes. This prooxidant action of AmO was corroborated in vitro in an adrenalin autooxidation model. On the other hand, the observed improved resistance to adrenalin stress in AmO supplemented rats was associated with an antioxidant response in blood and plasma membrane studies. The AmO effects can be attributed to the modulation of the metabolic pathways involved into oxygen and free radical homeostasis.
    Food Chemistry 03/2014; 147C:152-159. · 3.33 Impact Factor
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    ABSTRACT: Amaranth oil has several health benefits. It has lipid lowering, anti-diabetic, immune modulatory and cytoprotective properties, activates the function of mitochondria and improves heart rate variability. It has been suggested that the effect of amaranth oil on redox status is involved in this multitude of cellular and clinical influences of the oil. We examined whether amaranth oil can modify free radical production. EPR experiments with amaranth oil dissolved in DMSO showed scavenging of carbon centered radicals but not of oxygen centered radicals. Moreover, a concentration dependent scavenging effect of amaranth oil on ultrasound-induced radicals was observed. However, in adrenaline autoxidation experiments amaranth oil showed a strong prooxidant action through activation of superoxide anion formation. This two-sided effect of amaranth oil, i.e. both anti- and pro-oxidant action, was corroborated in human lung fibroblasts that were exposed to amaranth oil. At low concentrations of amaranth oil, fibroblasts were protected against oxidative stress, whereas in incubations with high amaranth oil concentrations more H2O2-induced intracellular radical damage was found. We suggest that mild pro-oxidant activity could be the underlying mechanism in the health beneficial effect of amaranth oil.
    Natural Products Chemistry & Research. 12/2013;
  • T. V. Sirota
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    ABSTRACT: The reaction of adrenaline autooxidation in an alkaline carbonate buffer followed by formation of superoxide radicals and the product of its oxidation, adrenochrome, which models the quinone pathway of adrenaline metabolism in the body, is accompanied by oxygen consumption. A study of this process by the polarographic method enabled one to apply this reaction to determine the activity of superoxide dismutase and antioxidant properties of biological and chemical compounds; it is based on evaluation of a latent period and the rate of oxygen consumption, which are measured in the presence of examined compounds. It was suggested that known neuroand cardiotoxicity of the quinone products of adrenaline oxidation may be associated not only to their intrinsic properties and reactive oxygen species formed but also local hypoxia of those regions of the cell and tissue where the quinone oxidation of adrenaline occurs. Keywordsadrenaline–adrenochrome–superoxide–oxygen–polarography–superoxide dismutase
    Biochemistry (Moscow) Supplement Series B Biomedical Chemistry 09/2011; 5(3):253-259.
  • Doklady Chemistry 01/2011; 437(1):60-62. · 0.39 Impact Factor
  • T V Sirota
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    ABSTRACT: The antioxidant system of some tissues in guinea pigs with no selenium deficiency was studied after treatment with selenium-containing products. Activity of a selenium-containing enzyme glutathione peroxidase was significantly reduced in the kidneys and blood. The kidneys were characterized by low activity of glutathione reductase and high activity of SOD. These features illustrate the development of oxidative stress in the kidneys.
    Bulletin of Experimental Biology and Medicine 10/2010; 149(4):412-5. · 0.34 Impact Factor
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    ABSTRACT: The effect of ionized air containing negatively charged ions at a concentration of 320000–350000 ions/cm3 inhaled by rats was studied. It was demonstrated that the inhalation of negative air ions for 60 min activated the secretion of goblet cells without impairing the tracheal mucosa and changing the protein profile of bronchoalveolar lavage. It was also found that the level of spontaneous production of reactive oxygen species by unfractionated blood cells increased after the action of negative air ions in both males and females. However, the intensity of their generation induced by opsonized zymosan increased only in females. Different sensitivities of the female and male blood antioxidant enzymes—superoxide dismutase and glutathione reductase—to negative air ions were observed. These results allow the effect of negative air ions on the respiratory organs and blood to be interpreted as priming and weak activation via a direct action on the mucosa of primary target respiratory organs and then on the blood.
    Biophysics 09/2008; 53(5):457-462.
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    ABSTRACT: Respiration parameters of liver mitochondria (MCh) in rats fed with amaranth seed oil for 3 weeks have been evaluated. Thirty minutes before decapitation, adrenaline was injected intraperitoneally at a low dose (350 μg/kg body weight) to both control and experimental animals. It was shown that in animals that were injected with adrenaline and did not receive oil, the rate of phosphorylating respiration increased by 32% and phosphorylation time decreased by 22% upon oxidation of succinate; upon oxidation of α-ketoglutarate in the presence of the succinate dehydrogenase inhibitor malonate, phosphorylating respiration was activated by 23%. The respiration of MCh upon oxidation of succinate + glutamate and α-ketoglutarate in the absence of malonate was not affected by adrenaline. The intake of oil markedly activated almost all parameters of mitochondrial respiration in experimental rats upon oxidation of all above-listed substrates in both coupled and uncoupled MCh. However, phosphorylation time was close to the control value (upon oxidation of succinate) or increased (upon oxidation of α-ketoglutarate in the presence and absence of malonate). The injection of adrenaline to animals receiving oil did not affect the oil-activated respiration of MCh oxidizing the substrates used; however, phosphorylation time in all groups of animals decreased. Ca2+ capacity of MCh in rats receiving amaranth oil did not change. Thus, our data show that feeding of rats with amaranth oil activates mitochondrial respiration and prevents MCh hyperactivation induced by adrenaline.
    Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology 02/2008; 2(1):40-47.
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    ABSTRACT: We studied behavioral reactions of rats after injection of subconvulsive dose of kainic acid into the frontal cortex and mitochondrial respiration in the hippocampus and frontal and temporal cortex 17-20 days after administration of kainic acid. Retention of acquired habit and the dynamics of its extinction in experimental rats were close to those in the control group. Changes in mitochondrial function were observed only in the region of kainic acid injection: activation of phosphorylating respiration during oxidation of succinate. Presumably, the detected activation of energy metabolism in the frontal cortex indicates functional restructuring in mitochondria, aimed at compensation of disorders caused by the neurotoxin.
    Bulletin of Experimental Biology and Medicine 12/2007; 144(5):670-3. · 0.34 Impact Factor
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    ABSTRACT: It has been shown that a three-week feeding of rats with oil derived from seeds of amaranth (Amaranthus cruentus L.) leads to a moderate activation of respiration of coupled and uncoupled rat liver mitochondria (MCh) that oxidize succinate and succinate + glutamate, as well as alpha-ketoglutarate and alpha-ketoglutarate + malonate. In animals receiving the amaranth oil, the injection of adrenaline did not affect the oil-activated respiration of MCh during succinate oxidation; i. e., animals prepared by an oil-enriched diet were resistant to the action of adrenaline, which prevented from possible hyperactivation of mitochondrial functions. In the group of control animals, which received no oil, the injection of adrenaline activated the rate of phosphorylating respiration of MCh during oxidation of succinate or succinate + glutamate: the rate of oxygen uptake in state 3 respiration (by Chance) increased, and the phosphorylation time decreased. The injection of adrenaline did not affect the parameters of respiration of MCh that oxidize a-ketoglutarate; however, in the presence of malonate, the oxidation of alpha-ketoglutarate in state 3 and uncoupled respiration have shown mild but significant increase in response to adrenaline. In animals receiving the amaranth oil, the oil-induced activation of respiration of MCh in response to adrenaline retained but did not increase; however, the phosphorylation time significantly decreased. Thus, concentrated oil of seeds activates the respiration of MCh. In addition, it enhances an energetic function of MCh, which prevents from the hyper-activation of mitochondrial respiration by adrenaline. Therefore an activation of energetic function of MCh by amaranth oil could explain its adaptogenic effect on rats.
    Ukrainskii biokhimicheskii zhurnal 01/2007; 79(5):196-203.
  • V I Arkhipov, T V Sirota, D S Lebedev
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    ABSTRACT: Cognitive processes and functional state of mitochondria in brain structures of Wistar rats were studied after intrahippocampal injection of kainic acid, an agonist of glutamate receptors. A single administration of 0.25 microg kainic acid into the dorsal part of the left and right hippocampi affected task retrieval and decreased inhibition of unrewarded responses. The injection of 0.75 microg kainic acid induced recurrent seizures and completely disorganized animal behavior. The functional state of mitochondria, as an important marker of excitotoxicity, was studied after intrahippocampal injections of kainic acid in the same doses. Kainic acid at 0.25 microg proved to activate the oxidative phosphorylation in hippocampal mitochondria. A higher (epileptogenic) dose of kainic acid inhibited mitochondrial respiration in the frontal cortex, but had an insignificant effect on mitochondrial respiration in the hippocampus. The disturbed interaction between the hippocampal system and frontal cortex after kainic acid administration can be the main factor of the revealed cognitive dysfunctions.
    Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk 01/2007;
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    ABSTRACT: It was shown for the first time that inhaling negative air ions (NAI) leads to changes in the functional state of the rat trachea mucosa, which in turn affects the phagocytic activity of blood cells. The effect depends on the concentration of the NAI, the duration of their action, and the sources of the NAI generation. For air ionization, technical ionizers and a bioionizer (a plant electrified through soil) developed by the Diod Company, Moscow, Russia were used. It was shown that the NAI at high doses (600 000 ion/cm<sup>3</sup>), generated by an Elion 131M device, induce a damage to the rat trachea mucosa, an inhibition of the activity of its antioxidant enzymes, and a decrease in the phagocytic activity of whole blood cells. Low doses of the NAI from the same source (25 000-50 000 ion/cm<sup>3</sup>) and high doses of the NAI from devices Elion 132S and 132R (320 000-500 000 ion/cm<sup>3</sup>), and a bioionizer (500 000 ion/cm<sup>3</sup>) produced a milder effect, activating the secretion of goblet cells of the trachea mucosa without its damage. A possible mechanism of the effect of inhaled NAI as exogenous reactive oxygen species (ROS) on internal processes in the organism is discussed. The involvement of the ROS in the action of ionized air found in the study is probably the general property of different electrical influences on biological objects. Therefore, the result of the investigation may be of interest for a wide circle of specialists dealing with ElectroMed influences
    IEEE Transactions on Plasma Science 09/2006; · 0.87 Impact Factor
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    ABSTRACT: We investigated the therapeutic effect of breathing ionized air in patients with chronic inflammation and decreased immune reactivity. Air ions were generated by an electroionizer, Tchijevsky Lustre. The air at the place of breathing contained 120 000 negative charges/cm<sup>3</sup>. By chemical nature, the negative air ions are superoxide at a concentration of less than 1 μM, and hydrogen peroxide at a concentration of about 1 μM. A three-week inhalation, for 1h daily, resulted in a decrease in the level of pro-inflammatory cytokines, the tumor necrosis factor (TNF), and interleukin-1 (IL-1), as well as excessive serotonin, while the level of the anti-inflammatory cytokine interferon tended to increase. These changes in inflammatory mediators are accompanied by a diminishing of the clinical manifestations of inflammation. The finding that NAI decrease the level of pro-inflammatory cytokines TNF and IL-1, and excessive serotonin explains the mechanism of their known curative effect in treatment of burns and opens a new area of their clinical applications for the treatment of other diseases related to the rise of TNF level, such as sepsis and even heart failure. The observed effects of negative air ions can be attributed to their ability to maintain a continuous flow of low concentration of H<sub>2</sub>O<sub>2</sub>, resulting from superoxide and serotonin oxidation. H<sub>2</sub>O<sub>2</sub> at a low concentration, is a necessary activator of the immune system.
    IEEE Transactions on Plasma Science 09/2004; · 0.87 Impact Factor
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    ABSTRACT: Generation of negative air ions (NAI) by pot plants (aloe Aloe arborescens, haworthia Haworthia rasalata, echinopsis Echinopsis tubiflora, mammillaria Mammillaria prolifera, opuntia Opuntia brunnescens, spider plant Chlorophytum comosum, and jade plant Crassula portulacea) was studied when high-voltage pulses were applied to soil. Plants that generated low amounts of NAI (such as jade plant and echinopsis) elevated NAI level 2–3 times over the mean background level. Plants that produced moderate amounts of NAI (haworthia, opuntia, and mammillaria) increased the NAI level hundredfold, whereas highly active plants (aloe and spider plant) increased the NAI level thousand times. Aloe plants can maintain constant NAI concentration in the ambient air (125 15 103 ions/cm3) for a long time (7–8 h). Negative air ions were predominantly generated by the leaf tips. The capacity of aloe to produce NAI considerably reduced with plant age and was lost, although reversibly, at temperatures below 8.5C. The rate of NAI generation considerably depended on weather conditions. It was demonstrated that superoxide anion radical is one of NAI species generated by plants.
    Russian Journal of Plant Physiology 04/2004; 51(3):414-419. · 0.62 Impact Factor
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    ABSTRACT: The role of impairment of general oxidative and energy metabolism in pathogenesis of lower urinary tract symptoms (LUTS) in patients with benign prostatic hyperplasia (BPH) and their correction by (1-adrenoblocker alfuzosin was studied. One group of patients (N = 126) was examined by standard methods for determination of the severity of LUTS by IPSS and mean effective volume of urinary bladder (MEVUB). In the second group (N = 29) in addition to functional examinations, metabolic indicators in blood were measured: antioxidant activity (AOA) and succinate dehydrogenase activity (SDA). Severity of LUTS depends greatly on the MEVUB. It was the first to show a practically complete correlation between LUTS, AOA and SDA. Severity of LUTS exactly correlates with indicators of oxidative and energy metabolism. In patients with more heavy LUTS, lowest AOA and SDA values were found. In the course of effective treatment, both phenomena developed an improvement of clinical symptoms and a rise of biochemical parameters. Close correlation between functional and metabolic phenomena is evidence of an essential role of metabolic mechanisms in the pathogenesis of LUTS with BPH. This opens perspectives to use antioxidants and energy metabolism activators for correction of UB dysfunction in patients with BPH.
    Mitochondrion 11/2003; 3(2):67-73. · 4.03 Impact Factor
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    ABSTRACT: It was shown that plantlets of wheat (Triticum vulgare) are capable of generating negative aeroions during the electrization of soil by high-voltage impulses. Soil electrization was carried out either from the moment of planting of seeds or from the appearance of the first seedlings. The concentration of negative ions was measured in the air at a distance of 50 cm from plants. In both variants, similar growth-related changes in the concentration of negative ions were observed. The generation of negative ions began on day 6 after the planting of seeds and reached a concentration of 380 x 10(3) ion/cm3. During the next three days, this level remained unchanged. On day 10, the generation of negative aeroions increased abruptly; on days 10-14, it was twofold as high as on days 7-9. The level of generation of negative aeroions by plants stimulated from the moment of appearance of plantlets was 5-8% higher than by plants stimulated from the moment of planting. The intensity of generation of negative aeroions upon additional illumination and in full darkness remained unchanged.
    Biofizika 01/2002; 47(1):130-4. · 0.43 Impact Factor
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    ABSTRACT: A protocol is developed for preparation of concentrated rat liver homogenate preserving assemblies of mitochondria in isotonic KCl under 0 and 15 degrees C. Assemblies preserve ability for self-organization during storage in homogenate. All key energy functions of mitochondria can be investigated in such a homogenate. Oxidative phosphorylation and membrane potential are stable for 5-7 h and can be still observed on the next day. Substrate-level phosphorylation is better pronounced for mitochondria in KCl than in sucrose medium while Ca2+ capacity is greater and lipid peroxidation is much lower. Sucrose addition impairs these functions. The rate of phosphorylating respiration is lower in large assemblies and higher in small. Transition from large to small assemblies corresponds to the transition from quiescent state of animal to adrenaline induced active state. The proposed method is particularly convenient for clinical investigations with small bioptates.
    Mitochondrion 11/2001; 1(3):249-67. · 4.03 Impact Factor
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    ABSTRACT: The primary chemical mechanism of the beneficial medical/biological action of negative air ions necessary for life was studied. Air ion deficiency is the cause of many illnesses and treatment with air ion inhalation is effective in many cases. However, its application is limited by the absence of knowledge of the primary mechanism of its action. The superoxide anion O<sub>2</sub><sup>-</sup> was detected in the flow of negative air ions generated by an electroeffluvial air ionizer. Earlier, the appearance of hydrogen peroxide in solutions treated with air ions was shown. The presence of these reactive oxygen species in ultralow and low concentrations (10<sup>-12</sup>-10<sup>-6</sup> M) suggested that the primary mechanism for the beneficial medical/biological action of negative air ions is moderate activation of free radical peroxidative oxidation within a physiological range that is lower than in tissues under pathology. It was shown in patients that treatment with inhalation of negative air ions did not induce pathological changes in superoxide dismutase activity and, under simultaneous administration of a food antioxidant, led to its fluid increase. The latter, along with some previous results, supports the proposed mechanism. In addition, taking the proposed mechanism into consideration, air ion doses for treatment can be selected on an individual basis and should depend on the redox state of the patient. This should achieve better results for medical treatment with ionized air
    IEEE Transactions on Plasma Science 03/2000; · 0.87 Impact Factor
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    ABSTRACT: A convenient model for studying the mechanisms of biological self-organization is described by morphometric investigation of formation of mitochondrion associations in medium containing physiological concentration of potassium ions without nonpolar substances. Association formation was considerably better at 15-18 degrees C during isolation and storage than at 0 degree C. The existence of filamented mitochondria in homogenate was also shown by staining of succinate dehydrogenase. Formation of associations increased in medium pretreated with negative air ions carrying superoxide and is probably due to hydrogen peroxide. The effect of substances influencing the surface charge on association formation was studied.
    Biofizika 01/2000; 45(1):83-8. · 0.43 Impact Factor
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    ABSTRACT: The effect of surfactants and micro dispersed solid oxides on the kinetics and mechanism of hydrocarbon (ethylbenzene, limonene, β-carotene) and lipid (sunflower oil triacylglycerols) liquid phase oxidation by molecular oxygen have been studied. Ionic surfactants sodium dodecylsulphate (SDS) and cetyltrimetyl ammonium bromide (CTAB) were found to affect the rate and mechanism of hydroperoxide decay and consequently the rate of the ethylbenzene and limonene oxidation. In the case of β-carotene, which does not form hydroperoxides in the course of oxidation; the surfactants do not affect the β-carotene consumption rate. Anionic surfactant SDS is found to be a catalyst for the heterolytic decay of hydroperoxides. In the case of α-phenyl ethyl hydroperoxide, the decay reaction catalyzed by SDS yields phenol that is an acceptor of free radicals. So the ethylbenzene oxidation is completely inhibited in the presence of SDS. The same effect on the ethylbenzene oxidation and its hydroperoxide decay was found to be caused by nanodispersed Al2O3. Cationic surfactant CTAB as a catalyst causes the decomposition of ethylbenzene and limonene hydroperoxides via free radical formation that results in acceleration of hydrocarbon oxidation. Solid oxides SiO2, TiO2 non-ionic ethoxylated hydrocarbons, and sodium bis (2-ethylhexyl)sulfosuccinate (AOT) show a slight effect on the hydroperoxide decay and hydrocarbon oxidation rates. The effect of surfactants and cosurfactants on the inhibited oxidation of lipid and hydrocarbons is strongly dependent on the nature of antioxidant and surfactant used.
    Colloids and Surfaces A Physicochemical and Engineering Aspects 04/1999; 149(s 1–3):29–38. · 2.11 Impact Factor
  • T V Sirota
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    ABSTRACT: The intensive rise of absorption maximum at 347 nm (wave number 28.8) was found during autoxidation of low concentrations of adrenaline (230 microM) in alkaline pH (10.65) under room temperature and in the absence of additional sources of superoxide. It was stated that the appearance of this product of adrenaline oxidation, which was not reported earlier, is considerably more rapid than formation of adrenochrome (absorbtion at 480 nm) and is inhibited by superoxide dismutase (SOD). It was shown that erythrocyte hemolysate and some studied antioxidants (ascorbate and cysteine) inhibit formation of this substance like SOD. The determination of the described substance can be used for measurement of both: activity of SOD or antioxidant activity of different biological materials. The possibility to use available not expensive chemicals (0.1% adrenaline solution from drug store, 0.2 M bicarbonate buffer) serves as an additional advantage of the proposed method.
    Voprosy medit͡sinskoĭ khimii 01/1999; 45(3):263-72.

Publication Stats

134 Citations
31.06 Total Impact Points

Institutions

  • 1999–2011
    • Russian Academy of Sciences
      • • Kurnakov Institute of General and Inorganic Chemistry
      • • N. N. Semenov Institute of Chemical Physics
      Moskva, Moscow, Russia
  • 1993–2010
    • Institute of Theoretical and Experimental Biophysics
      Pushchino-na-Oke, Moskovskaya, Russia
    • University of Helsinki
      • Department of Medical Chemistry
      Helsinki, Province of Southern Finland, Finland
  • 1998
    • Semenov Institute of Chemical Physics
      Moskva, Moscow, Russia